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Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress.

Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, Weimin H, Mingyu N, Jin H - Sci Rep (2015)

Bottom Line: Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06.Significant increase in photosynthetic pigment with PEG priming under stress.Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress.

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
The present study was designed to highlight the impact of seed priming with polyethylene glycol on physiological and molecular mechanism of two cultivars of Oryza sativa L. under different levels of zinc oxide nanorods (0, 250, 500 and 750 mg L(-1)). Plant growth parameters were significantly increased in seed priming with 30% PEG under nano-ZnO stress in both cultivars. Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Significant increase in photosynthetic pigment with PEG priming under stress. Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress. Gene expression analysis also suggested that expression of APXa, APXb, CATa, CATb, CATc, SOD1, SOD2 and SOD3 genes were down regulated with PEG priming as compared to non-primed seeds under stress. The ultrastructural analysis showed that leaf mesophyll and root cells were significantly damaged under nano-ZnO stress in both cultivars but the damage was prominent in Zhu Liang You 06. However, seed priming with PEG significantly alleviate the toxic effects of nano-ZnO stress and improved the cell structures of leaf and roots in both cultivars.

No MeSH data available.


Related in: MedlinePlus

Effects of seed priming with PEG (30%) on gene expressions of (A) SOD1, (B) SOD2 and (C) SOD3 in shoots and roots of two cultivars of Oryza sativa under different concentrations of nano-ZnO stress.
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f3: Effects of seed priming with PEG (30%) on gene expressions of (A) SOD1, (B) SOD2 and (C) SOD3 in shoots and roots of two cultivars of Oryza sativa under different concentrations of nano-ZnO stress.

Mentions: Significant up-regulation in SOD1 gene expression in root and shoot at all concentrations of nano-ZnO was observed (Fig. 3A). The highest expression of SOD1 gene was observed in shoot upon exposure to 750 mg L−1 in both cultivars as compared to non-stressed plants. However, seed priming with PEG significantly decreased the SOD1 expression under nano-ZnO stress conditions in both cultivars. This reduction in transcript levels of SOD1 gene was more clearly in root and shoot of cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Moreover, a significant increase in SOD2 expression was observed with increasing nano-ZnO concentration in both cultivars. Furthermore, data showed that seed priming with PEG induced decrease in transcription level of SOD2 in both rice cultivars as compared to control plants (Fig. 3B). Likewise, exposure to high stress of nano-ZnO induced significant up-regulation in SOD3 gene expression in both cultivars as compared to non-stressed plants. However, the up-regulation was clearly in root as compared to shoot irrespective of nano-ZnO concentrations (Fig. 3C). Results clearly described that seed priming with PEG induced decrease in SOD3 gene expression in both root and shoot of two cultivars irrespective of nano-ZnO concentration (Fig. 3C).


Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress.

Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, Weimin H, Mingyu N, Jin H - Sci Rep (2015)

Effects of seed priming with PEG (30%) on gene expressions of (A) SOD1, (B) SOD2 and (C) SOD3 in shoots and roots of two cultivars of Oryza sativa under different concentrations of nano-ZnO stress.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588511&req=5

f3: Effects of seed priming with PEG (30%) on gene expressions of (A) SOD1, (B) SOD2 and (C) SOD3 in shoots and roots of two cultivars of Oryza sativa under different concentrations of nano-ZnO stress.
Mentions: Significant up-regulation in SOD1 gene expression in root and shoot at all concentrations of nano-ZnO was observed (Fig. 3A). The highest expression of SOD1 gene was observed in shoot upon exposure to 750 mg L−1 in both cultivars as compared to non-stressed plants. However, seed priming with PEG significantly decreased the SOD1 expression under nano-ZnO stress conditions in both cultivars. This reduction in transcript levels of SOD1 gene was more clearly in root and shoot of cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Moreover, a significant increase in SOD2 expression was observed with increasing nano-ZnO concentration in both cultivars. Furthermore, data showed that seed priming with PEG induced decrease in transcription level of SOD2 in both rice cultivars as compared to control plants (Fig. 3B). Likewise, exposure to high stress of nano-ZnO induced significant up-regulation in SOD3 gene expression in both cultivars as compared to non-stressed plants. However, the up-regulation was clearly in root as compared to shoot irrespective of nano-ZnO concentrations (Fig. 3C). Results clearly described that seed priming with PEG induced decrease in SOD3 gene expression in both root and shoot of two cultivars irrespective of nano-ZnO concentration (Fig. 3C).

Bottom Line: Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06.Significant increase in photosynthetic pigment with PEG priming under stress.Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress.

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
The present study was designed to highlight the impact of seed priming with polyethylene glycol on physiological and molecular mechanism of two cultivars of Oryza sativa L. under different levels of zinc oxide nanorods (0, 250, 500 and 750 mg L(-1)). Plant growth parameters were significantly increased in seed priming with 30% PEG under nano-ZnO stress in both cultivars. Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Significant increase in photosynthetic pigment with PEG priming under stress. Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress. Gene expression analysis also suggested that expression of APXa, APXb, CATa, CATb, CATc, SOD1, SOD2 and SOD3 genes were down regulated with PEG priming as compared to non-primed seeds under stress. The ultrastructural analysis showed that leaf mesophyll and root cells were significantly damaged under nano-ZnO stress in both cultivars but the damage was prominent in Zhu Liang You 06. However, seed priming with PEG significantly alleviate the toxic effects of nano-ZnO stress and improved the cell structures of leaf and roots in both cultivars.

No MeSH data available.


Related in: MedlinePlus