Limits...
Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress.

Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, Weimin H, Mingyu N, Jin H - Sci Rep (2015)

Bottom Line: Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06.Significant increase in photosynthetic pigment with PEG priming under stress.Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress.

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
The present study was designed to highlight the impact of seed priming with polyethylene glycol on physiological and molecular mechanism of two cultivars of Oryza sativa L. under different levels of zinc oxide nanorods (0, 250, 500 and 750 mg L(-1)). Plant growth parameters were significantly increased in seed priming with 30% PEG under nano-ZnO stress in both cultivars. Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Significant increase in photosynthetic pigment with PEG priming under stress. Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress. Gene expression analysis also suggested that expression of APXa, APXb, CATa, CATb, CATc, SOD1, SOD2 and SOD3 genes were down regulated with PEG priming as compared to non-primed seeds under stress. The ultrastructural analysis showed that leaf mesophyll and root cells were significantly damaged under nano-ZnO stress in both cultivars but the damage was prominent in Zhu Liang You 06. However, seed priming with PEG significantly alleviate the toxic effects of nano-ZnO stress and improved the cell structures of leaf and roots in both cultivars.

No MeSH data available.


Related in: MedlinePlus

Effects of seed priming with PEG (30%) on gene expressions of (A) APXa, (B) APXb, (C) CATa, (D) CATb and (E) CATc in shoots and roots of two cultivars of Oryza sativa under different concentrations of nano-ZnO stress.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4588511&req=5

f2: Effects of seed priming with PEG (30%) on gene expressions of (A) APXa, (B) APXb, (C) CATa, (D) CATb and (E) CATc in shoots and roots of two cultivars of Oryza sativa under different concentrations of nano-ZnO stress.

Mentions: There was a significant difference in APXa expression in both root and shoot under different nano-ZnO concentrations. Significant up-regulation of APXa was observed in shoots after exposure to 500 and 750 mg L−1 nano-ZnO in both cultivars and the highest transcript levels of APXa was observed upon exposure to 750 mg L−1 as compared to non-stressed plants (Fig. 2A). Significant up-regulation of APXa was found in roots upon exposure to 500 and 750 mg L−1 nano-ZnO in both cultivars and this increase in transcript of APXa was more pronounced in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06 (p < 0.01) (Fig. 2A). The results indicated that seed priming with PEG (30%) induced significant reduction in transcript levels of APXa gene in both cultivars as compared with control (Fig. 2A). Similarly, exposure to different nano-ZnO concentrations induced a significant difference in APXb gene expression in both shoot and root of two cultivars. It was noticed that higher nano-ZnO concentration resulted in significant up-regulation in root and shoot of both cultivars (Fig. 2B). Irrespective of nano-ZnO concentrations, priming with PEG (30%) induced significant decrease in APXb gene expression in root and shoot of both cultivars as compared to the control (Fig. 2B).


Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress.

Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, Weimin H, Mingyu N, Jin H - Sci Rep (2015)

Effects of seed priming with PEG (30%) on gene expressions of (A) APXa, (B) APXb, (C) CATa, (D) CATb and (E) CATc in shoots and roots of two cultivars of Oryza sativa under different concentrations of nano-ZnO stress.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588511&req=5

f2: Effects of seed priming with PEG (30%) on gene expressions of (A) APXa, (B) APXb, (C) CATa, (D) CATb and (E) CATc in shoots and roots of two cultivars of Oryza sativa under different concentrations of nano-ZnO stress.
Mentions: There was a significant difference in APXa expression in both root and shoot under different nano-ZnO concentrations. Significant up-regulation of APXa was observed in shoots after exposure to 500 and 750 mg L−1 nano-ZnO in both cultivars and the highest transcript levels of APXa was observed upon exposure to 750 mg L−1 as compared to non-stressed plants (Fig. 2A). Significant up-regulation of APXa was found in roots upon exposure to 500 and 750 mg L−1 nano-ZnO in both cultivars and this increase in transcript of APXa was more pronounced in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06 (p < 0.01) (Fig. 2A). The results indicated that seed priming with PEG (30%) induced significant reduction in transcript levels of APXa gene in both cultivars as compared with control (Fig. 2A). Similarly, exposure to different nano-ZnO concentrations induced a significant difference in APXb gene expression in both shoot and root of two cultivars. It was noticed that higher nano-ZnO concentration resulted in significant up-regulation in root and shoot of both cultivars (Fig. 2B). Irrespective of nano-ZnO concentrations, priming with PEG (30%) induced significant decrease in APXb gene expression in root and shoot of both cultivars as compared to the control (Fig. 2B).

Bottom Line: Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06.Significant increase in photosynthetic pigment with PEG priming under stress.Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress.

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
The present study was designed to highlight the impact of seed priming with polyethylene glycol on physiological and molecular mechanism of two cultivars of Oryza sativa L. under different levels of zinc oxide nanorods (0, 250, 500 and 750 mg L(-1)). Plant growth parameters were significantly increased in seed priming with 30% PEG under nano-ZnO stress in both cultivars. Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Significant increase in photosynthetic pigment with PEG priming under stress. Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress. Gene expression analysis also suggested that expression of APXa, APXb, CATa, CATb, CATc, SOD1, SOD2 and SOD3 genes were down regulated with PEG priming as compared to non-primed seeds under stress. The ultrastructural analysis showed that leaf mesophyll and root cells were significantly damaged under nano-ZnO stress in both cultivars but the damage was prominent in Zhu Liang You 06. However, seed priming with PEG significantly alleviate the toxic effects of nano-ZnO stress and improved the cell structures of leaf and roots in both cultivars.

No MeSH data available.


Related in: MedlinePlus