Limits...
Unravelling polar lipids dynamics during embryonic development of two sympatric brachyuran crabs (Carcinus maenas and Necora puber) using lipidomics.

Rey F, Alves E, Melo T, Domingues P, Queiroga H, Rosa R, Domingues MR, Calado R - Sci Rep (2015)

Bottom Line: Throughout embryogenesis, phospholipids (PL) play a key role as an energy source, as well as constituents of biological membranes.The combination of thin layer chromatography, liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry allowed us to achieve an unprecedented resolution on PL classes and molecular species present on newly extruded embryos (stage 1) and those near hatching (stage 3).Embryogenesis proved to be a dynamic process, with four PL classes being recorded in stage 1 embryos (68 molecular species in total) and seven PL classes at stage 3 embryos (98 molecular species in total).

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biologia &CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT
Embryogenesis is an important stage of marine invertebrates with bi-phasic life cycles, as it conditions their larval and adult life. Throughout embryogenesis, phospholipids (PL) play a key role as an energy source, as well as constituents of biological membranes. However, the dynamics of PL during embryogenesis in marine invertebrates is still poorly studied. The present work used a lipidomic approach to determine how polar lipid profiles shift during embryogenesis in two sympatric estuarine crabs, Carcinus maenas and Necora puber. The combination of thin layer chromatography, liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry allowed us to achieve an unprecedented resolution on PL classes and molecular species present on newly extruded embryos (stage 1) and those near hatching (stage 3). Embryogenesis proved to be a dynamic process, with four PL classes being recorded in stage 1 embryos (68 molecular species in total) and seven PL classes at stage 3 embryos (98 molecular species in total). The low interspecific difference recorded in the lipidomic profiles of stage 1 embryos appears to indicate the existence of similar maternal investment. The same pattern was recorded for stage 3 embryos revealing a similar catabolism of embryonic resources during incubation for both crab species.

No MeSH data available.


Related in: MedlinePlus

(a) Relative abundance of phospholipids classes separated by thin layer chromatography in embryos at stage 1 and (b) at stage 3, and (c) fatty acid (FA) class profiles in embryos at stage 1 and (d) stage 3 of Carcinus maenas and Necora puber. Error bars represent standard deviation of three independent samples. P values for each significant statistical test performed are represented in the figure with a number, with significant differences between crab species being represented on the top of the graph bars and significant differences between stages of the same crab species being represented within the bar of stage 3 embryos. Significant interaction between species and stage is represented with an i on the top of stage 1 bars. (Post hoc Tukey HSD, 1: P = 0.0027; 2: P = 0.0095; 3: P = 0.0002; 4: P = 0.0001; 5: P = 0.0001; 6: P = 0.0266; 7: P = 0.0175; 8: P = 0.0361; 9: P = 0.0050). Interaction species vs stage (i): Epoxy FA (P = 0.0081). Abbreviations: PC - phosphatidylcholine; PE - phosphatidylethanolamine; SM - sphingomyelin; LysoPC - Lysophosphatidylcholine; CL - cardiolipin; PI - phosphatidylinositol SFA – Saturated FA; MUFA – Monounsaturated FA; PUFA – Polyunsaturated FA; HUFA – Highly-polyunsaturated FA; BrFA – Branched FA; CyFA – Cyclic FA; EpFA – Epoxy FA.
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f1: (a) Relative abundance of phospholipids classes separated by thin layer chromatography in embryos at stage 1 and (b) at stage 3, and (c) fatty acid (FA) class profiles in embryos at stage 1 and (d) stage 3 of Carcinus maenas and Necora puber. Error bars represent standard deviation of three independent samples. P values for each significant statistical test performed are represented in the figure with a number, with significant differences between crab species being represented on the top of the graph bars and significant differences between stages of the same crab species being represented within the bar of stage 3 embryos. Significant interaction between species and stage is represented with an i on the top of stage 1 bars. (Post hoc Tukey HSD, 1: P = 0.0027; 2: P = 0.0095; 3: P = 0.0002; 4: P = 0.0001; 5: P = 0.0001; 6: P = 0.0266; 7: P = 0.0175; 8: P = 0.0361; 9: P = 0.0050). Interaction species vs stage (i): Epoxy FA (P = 0.0081). Abbreviations: PC - phosphatidylcholine; PE - phosphatidylethanolamine; SM - sphingomyelin; LysoPC - Lysophosphatidylcholine; CL - cardiolipin; PI - phosphatidylinositol SFA – Saturated FA; MUFA – Monounsaturated FA; PUFA – Polyunsaturated FA; HUFA – Highly-polyunsaturated FA; BrFA – Branched FA; CyFA – Cyclic FA; EpFA – Epoxy FA.

Mentions: The fractioning of total lipid extracts in TLC plates showed two different profiles for each stage of embryogenesis (Fig. 1a,b). The separation by TLC enabled to identify phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) as the PL classes present in the initial stage of embryogenesis (Fig. 1a). LysoPC was observed at stage 1 embryos of C. maenas, but not on N. puber. At the end of embryonic development (stage 3) the separation by TLC revealed two new classes: phosphatidylinositol (PI) and cardiolipin (CL) (Fig. 1b). In both crab species and stages, the most abundant PL classes were PCs (C. maenas: 60.76 ± 4.28% and 40.15 ± 1.83%; N. puber: 74.13 ± 2.74% and 51.40 ± 3.58%, at stage 1 and 3, respectively) and PEs (C. maenas: 21.61 ± 1.33% and 20.89 ± 2.02%; N. puber: 19.63 ± 2.62% and 19.90 ± 2.94%, at stage 1 and 3, respectively).


Unravelling polar lipids dynamics during embryonic development of two sympatric brachyuran crabs (Carcinus maenas and Necora puber) using lipidomics.

Rey F, Alves E, Melo T, Domingues P, Queiroga H, Rosa R, Domingues MR, Calado R - Sci Rep (2015)

(a) Relative abundance of phospholipids classes separated by thin layer chromatography in embryos at stage 1 and (b) at stage 3, and (c) fatty acid (FA) class profiles in embryos at stage 1 and (d) stage 3 of Carcinus maenas and Necora puber. Error bars represent standard deviation of three independent samples. P values for each significant statistical test performed are represented in the figure with a number, with significant differences between crab species being represented on the top of the graph bars and significant differences between stages of the same crab species being represented within the bar of stage 3 embryos. Significant interaction between species and stage is represented with an i on the top of stage 1 bars. (Post hoc Tukey HSD, 1: P = 0.0027; 2: P = 0.0095; 3: P = 0.0002; 4: P = 0.0001; 5: P = 0.0001; 6: P = 0.0266; 7: P = 0.0175; 8: P = 0.0361; 9: P = 0.0050). Interaction species vs stage (i): Epoxy FA (P = 0.0081). Abbreviations: PC - phosphatidylcholine; PE - phosphatidylethanolamine; SM - sphingomyelin; LysoPC - Lysophosphatidylcholine; CL - cardiolipin; PI - phosphatidylinositol SFA – Saturated FA; MUFA – Monounsaturated FA; PUFA – Polyunsaturated FA; HUFA – Highly-polyunsaturated FA; BrFA – Branched FA; CyFA – Cyclic FA; EpFA – Epoxy FA.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4588508&req=5

f1: (a) Relative abundance of phospholipids classes separated by thin layer chromatography in embryos at stage 1 and (b) at stage 3, and (c) fatty acid (FA) class profiles in embryos at stage 1 and (d) stage 3 of Carcinus maenas and Necora puber. Error bars represent standard deviation of three independent samples. P values for each significant statistical test performed are represented in the figure with a number, with significant differences between crab species being represented on the top of the graph bars and significant differences between stages of the same crab species being represented within the bar of stage 3 embryos. Significant interaction between species and stage is represented with an i on the top of stage 1 bars. (Post hoc Tukey HSD, 1: P = 0.0027; 2: P = 0.0095; 3: P = 0.0002; 4: P = 0.0001; 5: P = 0.0001; 6: P = 0.0266; 7: P = 0.0175; 8: P = 0.0361; 9: P = 0.0050). Interaction species vs stage (i): Epoxy FA (P = 0.0081). Abbreviations: PC - phosphatidylcholine; PE - phosphatidylethanolamine; SM - sphingomyelin; LysoPC - Lysophosphatidylcholine; CL - cardiolipin; PI - phosphatidylinositol SFA – Saturated FA; MUFA – Monounsaturated FA; PUFA – Polyunsaturated FA; HUFA – Highly-polyunsaturated FA; BrFA – Branched FA; CyFA – Cyclic FA; EpFA – Epoxy FA.
Mentions: The fractioning of total lipid extracts in TLC plates showed two different profiles for each stage of embryogenesis (Fig. 1a,b). The separation by TLC enabled to identify phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) as the PL classes present in the initial stage of embryogenesis (Fig. 1a). LysoPC was observed at stage 1 embryos of C. maenas, but not on N. puber. At the end of embryonic development (stage 3) the separation by TLC revealed two new classes: phosphatidylinositol (PI) and cardiolipin (CL) (Fig. 1b). In both crab species and stages, the most abundant PL classes were PCs (C. maenas: 60.76 ± 4.28% and 40.15 ± 1.83%; N. puber: 74.13 ± 2.74% and 51.40 ± 3.58%, at stage 1 and 3, respectively) and PEs (C. maenas: 21.61 ± 1.33% and 20.89 ± 2.02%; N. puber: 19.63 ± 2.62% and 19.90 ± 2.94%, at stage 1 and 3, respectively).

Bottom Line: Throughout embryogenesis, phospholipids (PL) play a key role as an energy source, as well as constituents of biological membranes.The combination of thin layer chromatography, liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry allowed us to achieve an unprecedented resolution on PL classes and molecular species present on newly extruded embryos (stage 1) and those near hatching (stage 3).Embryogenesis proved to be a dynamic process, with four PL classes being recorded in stage 1 embryos (68 molecular species in total) and seven PL classes at stage 3 embryos (98 molecular species in total).

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biologia &CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT
Embryogenesis is an important stage of marine invertebrates with bi-phasic life cycles, as it conditions their larval and adult life. Throughout embryogenesis, phospholipids (PL) play a key role as an energy source, as well as constituents of biological membranes. However, the dynamics of PL during embryogenesis in marine invertebrates is still poorly studied. The present work used a lipidomic approach to determine how polar lipid profiles shift during embryogenesis in two sympatric estuarine crabs, Carcinus maenas and Necora puber. The combination of thin layer chromatography, liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry allowed us to achieve an unprecedented resolution on PL classes and molecular species present on newly extruded embryos (stage 1) and those near hatching (stage 3). Embryogenesis proved to be a dynamic process, with four PL classes being recorded in stage 1 embryos (68 molecular species in total) and seven PL classes at stage 3 embryos (98 molecular species in total). The low interspecific difference recorded in the lipidomic profiles of stage 1 embryos appears to indicate the existence of similar maternal investment. The same pattern was recorded for stage 3 embryos revealing a similar catabolism of embryonic resources during incubation for both crab species.

No MeSH data available.


Related in: MedlinePlus