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Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet.

Hernández-Salinas R, Decap V, Leguina A, Cáceres P, Perez D, Urquiaga I, Iturriaga R, Velarde V - Biol. Res. (2015)

Bottom Line: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group.We did not find any significant difference in body weight or systolic blood pressure in any of the groups.Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. romina.hernandez.salinas@gmail.com.

ABSTRACT

Background: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet.

Methods: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index was calculated using insulin and glucose values. A glucose tolerance test was performed 2 days before the end of the experiment. As an index of oxidative stress, thiobarbituric acid reactive substances (TBARS) level was measured in plasma and kidney, and superoxide dismutase was measured in the kidney.

Results: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group. In addition, the area under the curve of the glucose tolerance test was higher in HF fed animals. Furthermore, fasting blood glucose, plasma insulin levels, and the HOMA index, were also increased. WGP supplementation prevented these alterations in rats fed with the HF diet. We did not find any significant difference in body weight or systolic blood pressure in any of the groups.

Conclusions: Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet. We propose that WGP may be used as a supplement in human food as well.

No MeSH data available.


Related in: MedlinePlus

WGP prevented oxidative stress in renal tissue. Extracts from kidneys from control (C), control + WGP (C + WGP) high fructose (HF) and high fructose + WGP (HF + WGP) fed animals were used to determine TBARS. Bars represent mean ± SEM for n = 6–8 rats in each group, *P < 0.05 HF vs. other groups. Bonferroni after one way ANOVA
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Fig6: WGP prevented oxidative stress in renal tissue. Extracts from kidneys from control (C), control + WGP (C + WGP) high fructose (HF) and high fructose + WGP (HF + WGP) fed animals were used to determine TBARS. Bars represent mean ± SEM for n = 6–8 rats in each group, *P < 0.05 HF vs. other groups. Bonferroni after one way ANOVA

Mentions: To determine whether the changes in mSOD proteins levels correlated with the oxidative state found in the kidney, we measured TBARS in renal tissue. We found a significant increase in TBARS in the HF group, whereas this increase was prevented in the HF + WGP fed group (Fig. 6).Fig. 6


Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet.

Hernández-Salinas R, Decap V, Leguina A, Cáceres P, Perez D, Urquiaga I, Iturriaga R, Velarde V - Biol. Res. (2015)

WGP prevented oxidative stress in renal tissue. Extracts from kidneys from control (C), control + WGP (C + WGP) high fructose (HF) and high fructose + WGP (HF + WGP) fed animals were used to determine TBARS. Bars represent mean ± SEM for n = 6–8 rats in each group, *P < 0.05 HF vs. other groups. Bonferroni after one way ANOVA
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588460&req=5

Fig6: WGP prevented oxidative stress in renal tissue. Extracts from kidneys from control (C), control + WGP (C + WGP) high fructose (HF) and high fructose + WGP (HF + WGP) fed animals were used to determine TBARS. Bars represent mean ± SEM for n = 6–8 rats in each group, *P < 0.05 HF vs. other groups. Bonferroni after one way ANOVA
Mentions: To determine whether the changes in mSOD proteins levels correlated with the oxidative state found in the kidney, we measured TBARS in renal tissue. We found a significant increase in TBARS in the HF group, whereas this increase was prevented in the HF + WGP fed group (Fig. 6).Fig. 6

Bottom Line: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group.We did not find any significant difference in body weight or systolic blood pressure in any of the groups.Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. romina.hernandez.salinas@gmail.com.

ABSTRACT

Background: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet.

Methods: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index was calculated using insulin and glucose values. A glucose tolerance test was performed 2 days before the end of the experiment. As an index of oxidative stress, thiobarbituric acid reactive substances (TBARS) level was measured in plasma and kidney, and superoxide dismutase was measured in the kidney.

Results: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group. In addition, the area under the curve of the glucose tolerance test was higher in HF fed animals. Furthermore, fasting blood glucose, plasma insulin levels, and the HOMA index, were also increased. WGP supplementation prevented these alterations in rats fed with the HF diet. We did not find any significant difference in body weight or systolic blood pressure in any of the groups.

Conclusions: Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet. We propose that WGP may be used as a supplement in human food as well.

No MeSH data available.


Related in: MedlinePlus