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Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet.

Hernández-Salinas R, Decap V, Leguina A, Cáceres P, Perez D, Urquiaga I, Iturriaga R, Velarde V - Biol. Res. (2015)

Bottom Line: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group.We did not find any significant difference in body weight or systolic blood pressure in any of the groups.Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. romina.hernandez.salinas@gmail.com.

ABSTRACT

Background: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet.

Methods: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index was calculated using insulin and glucose values. A glucose tolerance test was performed 2 days before the end of the experiment. As an index of oxidative stress, thiobarbituric acid reactive substances (TBARS) level was measured in plasma and kidney, and superoxide dismutase was measured in the kidney.

Results: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group. In addition, the area under the curve of the glucose tolerance test was higher in HF fed animals. Furthermore, fasting blood glucose, plasma insulin levels, and the HOMA index, were also increased. WGP supplementation prevented these alterations in rats fed with the HF diet. We did not find any significant difference in body weight or systolic blood pressure in any of the groups.

Conclusions: Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet. We propose that WGP may be used as a supplement in human food as well.

No MeSH data available.


Related in: MedlinePlus

WGP prevents insulin resistance in HF fed rats. a Insulin was measured using a radioimmunoassay in control (C), control + WGP (C + WGP) high fructose (HF) and high fructose + WGP (HF + WGP) fed animals. b HOMA was calculated from insulin values shown in this figure and glucose values obtained at the same time points. Bars represent mean ± SEM for n = 6–8 rats in each group. *P < 0.05 HF vs. other groups. Bonferroni after one way ANOVA
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Fig3: WGP prevents insulin resistance in HF fed rats. a Insulin was measured using a radioimmunoassay in control (C), control + WGP (C + WGP) high fructose (HF) and high fructose + WGP (HF + WGP) fed animals. b HOMA was calculated from insulin values shown in this figure and glucose values obtained at the same time points. Bars represent mean ± SEM for n = 6–8 rats in each group. *P < 0.05 HF vs. other groups. Bonferroni after one way ANOVA

Mentions: To evaluate whether the increase in the AUC correlates with the insulin concentration, we measured insulin in the blood obtained after 12 h of starvation. Under these conditions, we found that rats from the HF fed group presented the highest levels of insulin, and its increase was prevented by the supplementation with WGP in the diet, as observed from the HF + WGP fed group (Fig. 3a). In addition, glucose levels were measured in these same blood samples and the homeostasis model assessment-estimated IR (HOMA-IR) index was evaluated for each group. HF fed rats presented a significantly higher HOMA-IR index compared to control rats. In addition, this increase was prevented in HF + WGP fed rats (Fig. 3b).Fig. 3


Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet.

Hernández-Salinas R, Decap V, Leguina A, Cáceres P, Perez D, Urquiaga I, Iturriaga R, Velarde V - Biol. Res. (2015)

WGP prevents insulin resistance in HF fed rats. a Insulin was measured using a radioimmunoassay in control (C), control + WGP (C + WGP) high fructose (HF) and high fructose + WGP (HF + WGP) fed animals. b HOMA was calculated from insulin values shown in this figure and glucose values obtained at the same time points. Bars represent mean ± SEM for n = 6–8 rats in each group. *P < 0.05 HF vs. other groups. Bonferroni after one way ANOVA
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588460&req=5

Fig3: WGP prevents insulin resistance in HF fed rats. a Insulin was measured using a radioimmunoassay in control (C), control + WGP (C + WGP) high fructose (HF) and high fructose + WGP (HF + WGP) fed animals. b HOMA was calculated from insulin values shown in this figure and glucose values obtained at the same time points. Bars represent mean ± SEM for n = 6–8 rats in each group. *P < 0.05 HF vs. other groups. Bonferroni after one way ANOVA
Mentions: To evaluate whether the increase in the AUC correlates with the insulin concentration, we measured insulin in the blood obtained after 12 h of starvation. Under these conditions, we found that rats from the HF fed group presented the highest levels of insulin, and its increase was prevented by the supplementation with WGP in the diet, as observed from the HF + WGP fed group (Fig. 3a). In addition, glucose levels were measured in these same blood samples and the homeostasis model assessment-estimated IR (HOMA-IR) index was evaluated for each group. HF fed rats presented a significantly higher HOMA-IR index compared to control rats. In addition, this increase was prevented in HF + WGP fed rats (Fig. 3b).Fig. 3

Bottom Line: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group.We did not find any significant difference in body weight or systolic blood pressure in any of the groups.Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. romina.hernandez.salinas@gmail.com.

ABSTRACT

Background: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet.

Methods: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index was calculated using insulin and glucose values. A glucose tolerance test was performed 2 days before the end of the experiment. As an index of oxidative stress, thiobarbituric acid reactive substances (TBARS) level was measured in plasma and kidney, and superoxide dismutase was measured in the kidney.

Results: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group. In addition, the area under the curve of the glucose tolerance test was higher in HF fed animals. Furthermore, fasting blood glucose, plasma insulin levels, and the HOMA index, were also increased. WGP supplementation prevented these alterations in rats fed with the HF diet. We did not find any significant difference in body weight or systolic blood pressure in any of the groups.

Conclusions: Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet. We propose that WGP may be used as a supplement in human food as well.

No MeSH data available.


Related in: MedlinePlus