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Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern.

Tabasi M, Asadi Karam MR, Habibi M, Yekaninejad MS, Bouzari S - Osong Public Health Res Perspect (2015)

Bottom Line: Our results also showed a relationship between biofilm formation in UPEC isolated from acute cystitis patients and recurrent UTI cases.We observed that the difference in antimicrobial susceptibilities of the biofilm and nonbiofilm former isolates was statistically significant.This study indicated that there is a relationship between the phenotypic virulence traits of the UPEC isolates, patients' profiles, and antibiotic resistance.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.

ABSTRACT

Objectives: Urinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains is one of the most important infections in the world. UPEC encode widespread virulence factors closely related with pathogenesis of the bacteria. The purpose of this study was to evaluate the presence of different phenotypic virulence markers in UPEC isolates and determine their correlation with antibiotic resistance pattern.

Methods: UPEC isolates from patients with different clinical symptoms of UTI were collected and screened for biofilm and hemolysin production, mannose resistant, and mannose sensitive hemagglutination (MRHA and MSHA, respectively). In addition, antimicrobial resistance pattern and ESBL-producing isolates were recorded.

Results: Of the 156 UPEC isolates, biofilm and hemolysin formation was seen in 133 (85.3%) and 53 (34%) isolates, respectively. Moreover, 98 (62.8%) and 58 (37.2%) isolates showed the presence of Types 1 fimbriae (MSHA) and P fimbriae (MRHA), respectively. Our results also showed a relationship between biofilm formation in UPEC isolated from acute cystitis patients and recurrent UTI cases. Occurrence of UTI was dramatically correlated with the patients' profiles. We observed that the difference in antimicrobial susceptibilities of the biofilm and nonbiofilm former isolates was statistically significant. The UPEC isolates showed the highest resistance to ampicillin, tetracycline, amoxicillin, and cotrimoxazole. Moreover, 26.9% of isolates were ESBL producers.

Conclusion: This study indicated that there is a relationship between the phenotypic virulence traits of the UPEC isolates, patients' profiles, and antibiotic resistance. Detection of the phenotypic virulence factors could help to improve understanding of pathogenesis of UPEC isolates and better medical intervention.

No MeSH data available.


Related in: MedlinePlus

Antimicrobial resistance pattern of UPEC isolates from patients with urinary tract infections (UTI). UPEC = uropathogenic Escherichia coli.
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fig1: Antimicrobial resistance pattern of UPEC isolates from patients with urinary tract infections (UTI). UPEC = uropathogenic Escherichia coli.

Mentions: The resistance pattern of UPEC isolates to the different antimicrobial agents is shown in Fig. 1. Among the antibiotics tested, ampicillin resistance prevalence was the highest (77.6%), followed by tetracycline (60.3%), amoxicillin (59%), cotrimoxazole (58.3%), and piperacillin (55.8%). The isolates showed the highest sensitivity to antibiotics such as imipenem and meropenem (100%), amikacin (96.8%), piperacillin/tazobactum, and nitrofurantion (94.9%). Double disc synergy test (DDST) showed that 26.9% of the UPEC isolates were positive for ESBL production. Totally, 123 isolates tested (79%) were multidrug-resistant (isolates with resistance to three or more different classes of antibiotics).


Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern.

Tabasi M, Asadi Karam MR, Habibi M, Yekaninejad MS, Bouzari S - Osong Public Health Res Perspect (2015)

Antimicrobial resistance pattern of UPEC isolates from patients with urinary tract infections (UTI). UPEC = uropathogenic Escherichia coli.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588432&req=5

fig1: Antimicrobial resistance pattern of UPEC isolates from patients with urinary tract infections (UTI). UPEC = uropathogenic Escherichia coli.
Mentions: The resistance pattern of UPEC isolates to the different antimicrobial agents is shown in Fig. 1. Among the antibiotics tested, ampicillin resistance prevalence was the highest (77.6%), followed by tetracycline (60.3%), amoxicillin (59%), cotrimoxazole (58.3%), and piperacillin (55.8%). The isolates showed the highest sensitivity to antibiotics such as imipenem and meropenem (100%), amikacin (96.8%), piperacillin/tazobactum, and nitrofurantion (94.9%). Double disc synergy test (DDST) showed that 26.9% of the UPEC isolates were positive for ESBL production. Totally, 123 isolates tested (79%) were multidrug-resistant (isolates with resistance to three or more different classes of antibiotics).

Bottom Line: Our results also showed a relationship between biofilm formation in UPEC isolated from acute cystitis patients and recurrent UTI cases.We observed that the difference in antimicrobial susceptibilities of the biofilm and nonbiofilm former isolates was statistically significant.This study indicated that there is a relationship between the phenotypic virulence traits of the UPEC isolates, patients' profiles, and antibiotic resistance.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.

ABSTRACT

Objectives: Urinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains is one of the most important infections in the world. UPEC encode widespread virulence factors closely related with pathogenesis of the bacteria. The purpose of this study was to evaluate the presence of different phenotypic virulence markers in UPEC isolates and determine their correlation with antibiotic resistance pattern.

Methods: UPEC isolates from patients with different clinical symptoms of UTI were collected and screened for biofilm and hemolysin production, mannose resistant, and mannose sensitive hemagglutination (MRHA and MSHA, respectively). In addition, antimicrobial resistance pattern and ESBL-producing isolates were recorded.

Results: Of the 156 UPEC isolates, biofilm and hemolysin formation was seen in 133 (85.3%) and 53 (34%) isolates, respectively. Moreover, 98 (62.8%) and 58 (37.2%) isolates showed the presence of Types 1 fimbriae (MSHA) and P fimbriae (MRHA), respectively. Our results also showed a relationship between biofilm formation in UPEC isolated from acute cystitis patients and recurrent UTI cases. Occurrence of UTI was dramatically correlated with the patients' profiles. We observed that the difference in antimicrobial susceptibilities of the biofilm and nonbiofilm former isolates was statistically significant. The UPEC isolates showed the highest resistance to ampicillin, tetracycline, amoxicillin, and cotrimoxazole. Moreover, 26.9% of isolates were ESBL producers.

Conclusion: This study indicated that there is a relationship between the phenotypic virulence traits of the UPEC isolates, patients' profiles, and antibiotic resistance. Detection of the phenotypic virulence factors could help to improve understanding of pathogenesis of UPEC isolates and better medical intervention.

No MeSH data available.


Related in: MedlinePlus