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A short report: PAMM, a novel antioxidant protein, induced by oxidative stress.

Xu Y, Morse LR, da Silva RA, Wang D, Battaglino RA - Redox Biol (2015)

Bottom Line: These results indicate that M-CSF-induced PAMM expression is mediated by Akt phosphorylation.Our data also suggest that estrogen-induced PAMM expression is mediated by phosphorylation of Akt.These findings point to PAMM as a potential candidate for Akt-mediated protection against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Mineralized Tissue Biology, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA; School of Pharmaceutical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, China. Electronic address: yxu@forsyth.org.

No MeSH data available.


Related in: MedlinePlus

Antioxidant stress induces PAMM mRNA expression in human PBMCs. Human peripheral blood mononuclear cells (PBMCs) were treated with increasing concentrations of H2O2 (0, 10, 20, 50 and 100 µM) for 20 min. Total RNA was extracted 24 h after treatment, reverse transcribed into cDNA and analyzed by real-time PCR. (A) PAMM mRNA abundance is significantly increased by treatment with 100 µM H2O2 in human PBMC. (B) Nrf2 mRNA abundance is significantly increased by treatment with 50 and 100 µM H2O2 in human PBMC compared with untreated cells.
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f0025: Antioxidant stress induces PAMM mRNA expression in human PBMCs. Human peripheral blood mononuclear cells (PBMCs) were treated with increasing concentrations of H2O2 (0, 10, 20, 50 and 100 µM) for 20 min. Total RNA was extracted 24 h after treatment, reverse transcribed into cDNA and analyzed by real-time PCR. (A) PAMM mRNA abundance is significantly increased by treatment with 100 µM H2O2 in human PBMC. (B) Nrf2 mRNA abundance is significantly increased by treatment with 50 and 100 µM H2O2 in human PBMC compared with untreated cells.

Mentions: Ovariectomy causes both estrogen-deficiency and oxidative stress [5]. We observed increased PAMM expression in long bones of mice from the OVX/Vehicle group suggesting that upregulation of PAMM was induced by oxidative stress in vivo. To confirm this observation at the cellular level, we treated human PBMCs with H2O2 for 20 min, after which we replaced the H2O2-containing medium with the fresh medium without H2O2[17,18]. Total RNA was extracted at 24 h after treatment and analyzed for PAMM mRNA expression by RT PCR. We found significantly increased PAMM mRNA abundance in human PBMCs treated with 100 µM H2O2, (Fig. 5A). Nuclear factor-erythroid 2-related factor 2 (Nrf2) is a transcription factor that regulates the cellular response to oxidative stress by stimulating expression of several antioxidant proteins [19]. We hypothesized that H2O2-induced PAMM expression was associated with H2O2-induced Nrf2 mRNA expression. Indeed, we found a significant increase in Nrf2 mRNA in cells treated with 50 µM H2O2 (Fig. 5B), confirming our hypothesis.


A short report: PAMM, a novel antioxidant protein, induced by oxidative stress.

Xu Y, Morse LR, da Silva RA, Wang D, Battaglino RA - Redox Biol (2015)

Antioxidant stress induces PAMM mRNA expression in human PBMCs. Human peripheral blood mononuclear cells (PBMCs) were treated with increasing concentrations of H2O2 (0, 10, 20, 50 and 100 µM) for 20 min. Total RNA was extracted 24 h after treatment, reverse transcribed into cDNA and analyzed by real-time PCR. (A) PAMM mRNA abundance is significantly increased by treatment with 100 µM H2O2 in human PBMC. (B) Nrf2 mRNA abundance is significantly increased by treatment with 50 and 100 µM H2O2 in human PBMC compared with untreated cells.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4588419&req=5

f0025: Antioxidant stress induces PAMM mRNA expression in human PBMCs. Human peripheral blood mononuclear cells (PBMCs) were treated with increasing concentrations of H2O2 (0, 10, 20, 50 and 100 µM) for 20 min. Total RNA was extracted 24 h after treatment, reverse transcribed into cDNA and analyzed by real-time PCR. (A) PAMM mRNA abundance is significantly increased by treatment with 100 µM H2O2 in human PBMC. (B) Nrf2 mRNA abundance is significantly increased by treatment with 50 and 100 µM H2O2 in human PBMC compared with untreated cells.
Mentions: Ovariectomy causes both estrogen-deficiency and oxidative stress [5]. We observed increased PAMM expression in long bones of mice from the OVX/Vehicle group suggesting that upregulation of PAMM was induced by oxidative stress in vivo. To confirm this observation at the cellular level, we treated human PBMCs with H2O2 for 20 min, after which we replaced the H2O2-containing medium with the fresh medium without H2O2[17,18]. Total RNA was extracted at 24 h after treatment and analyzed for PAMM mRNA expression by RT PCR. We found significantly increased PAMM mRNA abundance in human PBMCs treated with 100 µM H2O2, (Fig. 5A). Nuclear factor-erythroid 2-related factor 2 (Nrf2) is a transcription factor that regulates the cellular response to oxidative stress by stimulating expression of several antioxidant proteins [19]. We hypothesized that H2O2-induced PAMM expression was associated with H2O2-induced Nrf2 mRNA expression. Indeed, we found a significant increase in Nrf2 mRNA in cells treated with 50 µM H2O2 (Fig. 5B), confirming our hypothesis.

Bottom Line: These results indicate that M-CSF-induced PAMM expression is mediated by Akt phosphorylation.Our data also suggest that estrogen-induced PAMM expression is mediated by phosphorylation of Akt.These findings point to PAMM as a potential candidate for Akt-mediated protection against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Mineralized Tissue Biology, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA; School of Pharmaceutical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, China. Electronic address: yxu@forsyth.org.

No MeSH data available.


Related in: MedlinePlus