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A short report: PAMM, a novel antioxidant protein, induced by oxidative stress.

Xu Y, Morse LR, da Silva RA, Wang D, Battaglino RA - Redox Biol (2015)

Bottom Line: These results indicate that M-CSF-induced PAMM expression is mediated by Akt phosphorylation.Our data also suggest that estrogen-induced PAMM expression is mediated by phosphorylation of Akt.These findings point to PAMM as a potential candidate for Akt-mediated protection against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Mineralized Tissue Biology, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA; School of Pharmaceutical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, China. Electronic address: yxu@forsyth.org.

No MeSH data available.


Related in: MedlinePlus

Ovariectomy increases PAMM abundance and Akt phosphorylation in bone. (A) Western blot analysis of protein extracts from distal femur shows increased PAMM expression and Akt phosphorylation in OVX/Vehicle group (n=3, lanes 1, 2, and 3) and OVX/E2 group (n=2, lanes 4 and 5) compared to Sham/Vehicle mice (n=3, lanes 6, 7, and 8). (B) PAMM and pAkt are increased in OVX group compare with Sham group (*p<0.05). There is a trend toward increased PAMM expression with E2 treatment in OVX mice. (C) Expression of PAMM at the distal femur was also detected by immunohistochemistry 4 weeks after OVX or Sham-operated mice.
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f0010: Ovariectomy increases PAMM abundance and Akt phosphorylation in bone. (A) Western blot analysis of protein extracts from distal femur shows increased PAMM expression and Akt phosphorylation in OVX/Vehicle group (n=3, lanes 1, 2, and 3) and OVX/E2 group (n=2, lanes 4 and 5) compared to Sham/Vehicle mice (n=3, lanes 6, 7, and 8). (B) PAMM and pAkt are increased in OVX group compare with Sham group (*p<0.05). There is a trend toward increased PAMM expression with E2 treatment in OVX mice. (C) Expression of PAMM at the distal femur was also detected by immunohistochemistry 4 weeks after OVX or Sham-operated mice.

Mentions: Activation of the PI3K/Akt/NF-κB signaling pathway is essential for osteoclast differentiation from monocytic precursors, osteoclast proliferation, osteoclast survival, and osteoclastic bone resorbing activity [12,13,16]. Akt also provides a survival signal that protects cells from apoptosis induced by growth factor withdrawal and oxidative stress [14]. We hypothesized that ovariectomy would result in both Akt activation and increased PAMM expression in bone. We therefore determined Akt activation in bone by assessing Ser 473 phosphorylation status by western blot analysis. We detected increased Akt phosphorylation in protein lysates from the distal femur in the OVX/Vehicle and OVX/E2 groups compared to the Sham/Vehicle group (p<0.05). Similarly, we found significantly increased PAMM protein expression in the OVX/Vehicle and OVX/E2 groups compared to the Sham/Vehicle group (p<0.05) (Fig. 2A and B). Expression of PAMM at the distal femur was also detected by immunohistochemistry 4 weeks after OVX or Sham-operated mice (Fig. 2C).


A short report: PAMM, a novel antioxidant protein, induced by oxidative stress.

Xu Y, Morse LR, da Silva RA, Wang D, Battaglino RA - Redox Biol (2015)

Ovariectomy increases PAMM abundance and Akt phosphorylation in bone. (A) Western blot analysis of protein extracts from distal femur shows increased PAMM expression and Akt phosphorylation in OVX/Vehicle group (n=3, lanes 1, 2, and 3) and OVX/E2 group (n=2, lanes 4 and 5) compared to Sham/Vehicle mice (n=3, lanes 6, 7, and 8). (B) PAMM and pAkt are increased in OVX group compare with Sham group (*p<0.05). There is a trend toward increased PAMM expression with E2 treatment in OVX mice. (C) Expression of PAMM at the distal femur was also detected by immunohistochemistry 4 weeks after OVX or Sham-operated mice.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4588419&req=5

f0010: Ovariectomy increases PAMM abundance and Akt phosphorylation in bone. (A) Western blot analysis of protein extracts from distal femur shows increased PAMM expression and Akt phosphorylation in OVX/Vehicle group (n=3, lanes 1, 2, and 3) and OVX/E2 group (n=2, lanes 4 and 5) compared to Sham/Vehicle mice (n=3, lanes 6, 7, and 8). (B) PAMM and pAkt are increased in OVX group compare with Sham group (*p<0.05). There is a trend toward increased PAMM expression with E2 treatment in OVX mice. (C) Expression of PAMM at the distal femur was also detected by immunohistochemistry 4 weeks after OVX or Sham-operated mice.
Mentions: Activation of the PI3K/Akt/NF-κB signaling pathway is essential for osteoclast differentiation from monocytic precursors, osteoclast proliferation, osteoclast survival, and osteoclastic bone resorbing activity [12,13,16]. Akt also provides a survival signal that protects cells from apoptosis induced by growth factor withdrawal and oxidative stress [14]. We hypothesized that ovariectomy would result in both Akt activation and increased PAMM expression in bone. We therefore determined Akt activation in bone by assessing Ser 473 phosphorylation status by western blot analysis. We detected increased Akt phosphorylation in protein lysates from the distal femur in the OVX/Vehicle and OVX/E2 groups compared to the Sham/Vehicle group (p<0.05). Similarly, we found significantly increased PAMM protein expression in the OVX/Vehicle and OVX/E2 groups compared to the Sham/Vehicle group (p<0.05) (Fig. 2A and B). Expression of PAMM at the distal femur was also detected by immunohistochemistry 4 weeks after OVX or Sham-operated mice (Fig. 2C).

Bottom Line: These results indicate that M-CSF-induced PAMM expression is mediated by Akt phosphorylation.Our data also suggest that estrogen-induced PAMM expression is mediated by phosphorylation of Akt.These findings point to PAMM as a potential candidate for Akt-mediated protection against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Mineralized Tissue Biology, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA; School of Pharmaceutical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, China. Electronic address: yxu@forsyth.org.

No MeSH data available.


Related in: MedlinePlus