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A short report: PAMM, a novel antioxidant protein, induced by oxidative stress.

Xu Y, Morse LR, da Silva RA, Wang D, Battaglino RA - Redox Biol (2015)

Bottom Line: These results indicate that M-CSF-induced PAMM expression is mediated by Akt phosphorylation.Our data also suggest that estrogen-induced PAMM expression is mediated by phosphorylation of Akt.These findings point to PAMM as a potential candidate for Akt-mediated protection against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Mineralized Tissue Biology, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA; School of Pharmaceutical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, China. Electronic address: yxu@forsyth.org.

No MeSH data available.


Related in: MedlinePlus

Bone loss and oxidative stress are induced by ovariectomy. (A) Bone density (distal femur and whole body) was determined by DXA immediately prior to surgery and 4 weeks after surgery. Decreased BMD was determined in the OVX/Vehicle group compared to the OVX/E2 and Sham/Vehicle groups (**p<0.01). (B) TRAP staining was performed on sections through the distal femur from OVX and sham-operated animals with/without estrogen replacement to quantify osteoclasts (TRAP positive cells). (C) Osteoclasts were counted per area (40X) in all groups. Results are expressed as mean +/− SD. We detected fewer osteoclasts in the OVX/E2 and SHAM groups (**p<0.01) compared to the OVX/Vehicle group. (D) Decreased total GSH (**p<0.01) and GSH/GSSG (*p<0.05) ratio from bone protein lysates is detected in the OVX/Vehicle group showing that oxidative stress in OVX mice.
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f0005: Bone loss and oxidative stress are induced by ovariectomy. (A) Bone density (distal femur and whole body) was determined by DXA immediately prior to surgery and 4 weeks after surgery. Decreased BMD was determined in the OVX/Vehicle group compared to the OVX/E2 and Sham/Vehicle groups (**p<0.01). (B) TRAP staining was performed on sections through the distal femur from OVX and sham-operated animals with/without estrogen replacement to quantify osteoclasts (TRAP positive cells). (C) Osteoclasts were counted per area (40X) in all groups. Results are expressed as mean +/− SD. We detected fewer osteoclasts in the OVX/E2 and SHAM groups (**p<0.01) compared to the OVX/Vehicle group. (D) Decreased total GSH (**p<0.01) and GSH/GSSG (*p<0.05) ratio from bone protein lysates is detected in the OVX/Vehicle group showing that oxidative stress in OVX mice.

Mentions: Distal femur bone mineral density (BMD) and whole body BMD (excluding the head) were assessed in all groups immediately prior to OVX/Sham and 4 weeks after OVX/Sham. There were no significant differences in baseline distal femur or total body BMD between the groups (p>0.05). Over the 4-week period, distal femur BMD increased significantly in the OVX/E2 and the Sham/Vehicle groups (70.5% and 79%, respectively) while distal femur BMD decreased significantly in the OVX/Vehicle group (9.7%). Similarly, there was no detectable difference in whole body BMD in the OVX/E2 and the Sham/Vehicle groups while there was a significant decline in the OVX/Vehicle group. As a result, both distal femur and total body BMD were significantly lower in the OVX/Vehicle group (0.068 g/cm2 and 0.060 g/cm2, respectively) compared to the OVX/E2 (0.125 g/cm2 and 0.065 g/cm2) and Sham/Vehicle group (0.134 g/cm2 and 0.065 g/cm2) after 4 weeks of treatment (**p<0.01) (Fig. 1A). TRAP staining was performed of sections through the distal femur and osteoclasts were quantified as the number of TRAP positive cells per high-powered field (Fig. 1B). There were significantly fewer osteoclasts in the OVX/E2 and SHAM/Vehicle groups compared to the OVX/Vehicle group (**p<0.01, Fig. 1C) confirming that estrogen replacement inhibited ovariectomy-induced osteoclast differentiation. Moreover, decreased GSH(t) and GSH/GSSG ratio were detected in the OVX/Vehicle group (p<0.05, Fig. 1D), confirming oxidative stress in ovariectomized mice.


A short report: PAMM, a novel antioxidant protein, induced by oxidative stress.

Xu Y, Morse LR, da Silva RA, Wang D, Battaglino RA - Redox Biol (2015)

Bone loss and oxidative stress are induced by ovariectomy. (A) Bone density (distal femur and whole body) was determined by DXA immediately prior to surgery and 4 weeks after surgery. Decreased BMD was determined in the OVX/Vehicle group compared to the OVX/E2 and Sham/Vehicle groups (**p<0.01). (B) TRAP staining was performed on sections through the distal femur from OVX and sham-operated animals with/without estrogen replacement to quantify osteoclasts (TRAP positive cells). (C) Osteoclasts were counted per area (40X) in all groups. Results are expressed as mean +/− SD. We detected fewer osteoclasts in the OVX/E2 and SHAM groups (**p<0.01) compared to the OVX/Vehicle group. (D) Decreased total GSH (**p<0.01) and GSH/GSSG (*p<0.05) ratio from bone protein lysates is detected in the OVX/Vehicle group showing that oxidative stress in OVX mice.
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Related In: Results  -  Collection

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f0005: Bone loss and oxidative stress are induced by ovariectomy. (A) Bone density (distal femur and whole body) was determined by DXA immediately prior to surgery and 4 weeks after surgery. Decreased BMD was determined in the OVX/Vehicle group compared to the OVX/E2 and Sham/Vehicle groups (**p<0.01). (B) TRAP staining was performed on sections through the distal femur from OVX and sham-operated animals with/without estrogen replacement to quantify osteoclasts (TRAP positive cells). (C) Osteoclasts were counted per area (40X) in all groups. Results are expressed as mean +/− SD. We detected fewer osteoclasts in the OVX/E2 and SHAM groups (**p<0.01) compared to the OVX/Vehicle group. (D) Decreased total GSH (**p<0.01) and GSH/GSSG (*p<0.05) ratio from bone protein lysates is detected in the OVX/Vehicle group showing that oxidative stress in OVX mice.
Mentions: Distal femur bone mineral density (BMD) and whole body BMD (excluding the head) were assessed in all groups immediately prior to OVX/Sham and 4 weeks after OVX/Sham. There were no significant differences in baseline distal femur or total body BMD between the groups (p>0.05). Over the 4-week period, distal femur BMD increased significantly in the OVX/E2 and the Sham/Vehicle groups (70.5% and 79%, respectively) while distal femur BMD decreased significantly in the OVX/Vehicle group (9.7%). Similarly, there was no detectable difference in whole body BMD in the OVX/E2 and the Sham/Vehicle groups while there was a significant decline in the OVX/Vehicle group. As a result, both distal femur and total body BMD were significantly lower in the OVX/Vehicle group (0.068 g/cm2 and 0.060 g/cm2, respectively) compared to the OVX/E2 (0.125 g/cm2 and 0.065 g/cm2) and Sham/Vehicle group (0.134 g/cm2 and 0.065 g/cm2) after 4 weeks of treatment (**p<0.01) (Fig. 1A). TRAP staining was performed of sections through the distal femur and osteoclasts were quantified as the number of TRAP positive cells per high-powered field (Fig. 1B). There were significantly fewer osteoclasts in the OVX/E2 and SHAM/Vehicle groups compared to the OVX/Vehicle group (**p<0.01, Fig. 1C) confirming that estrogen replacement inhibited ovariectomy-induced osteoclast differentiation. Moreover, decreased GSH(t) and GSH/GSSG ratio were detected in the OVX/Vehicle group (p<0.05, Fig. 1D), confirming oxidative stress in ovariectomized mice.

Bottom Line: These results indicate that M-CSF-induced PAMM expression is mediated by Akt phosphorylation.Our data also suggest that estrogen-induced PAMM expression is mediated by phosphorylation of Akt.These findings point to PAMM as a potential candidate for Akt-mediated protection against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Mineralized Tissue Biology, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA; School of Pharmaceutical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, China. Electronic address: yxu@forsyth.org.

No MeSH data available.


Related in: MedlinePlus