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Ellagic acid, a polyphenolic compound, selectively induces ROS-mediated apoptosis in cancerous B-lymphocytes of CLL patients by directly targeting mitochondria.

Salimi A, Roudkenar MH, Sadeghi L, Mohseni A, Seydi E, Pirahmadi N, Pourahmad J - Redox Biol (2015)

Bottom Line: Based on our results EA decreased the percentage of viable cells and induced apoptosis.EA increased ROS formation, mitochondria swelling, MMP decrease and cytochrome c release in mitochondria isolated from CLL BUT NOT healthy B-lymphocytes while pre-treatment with cyclosporine A and Butylated hydroxyl toluene (BHT) prevented these effects.Our results suggest that EA can act as an anti cancer candidate by directly and selectively targeting mitochondria could induce apoptosis through mitochondria pathway with increasing ROS production which finally ends in cytochrome c release, caspase 3 activation and apoptosis in cancerous B-lymphocytes isolated from CLL patients.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

No MeSH data available.


Related in: MedlinePlus

Induction of mitochondrial swelling by EA. Addition of EA (5, 10 and 20 µM) induces mitochondrial swelling in CLL BUT NOT in healthy B lymphocyte mitochondria in a concentration depending manner. Mitochondria were suspended in swelling buffer and incubated for 1 h. The mitochondrial swelling was measured by following absorbance (λmax=540 nm) decrease.
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f0045: Induction of mitochondrial swelling by EA. Addition of EA (5, 10 and 20 µM) induces mitochondrial swelling in CLL BUT NOT in healthy B lymphocyte mitochondria in a concentration depending manner. Mitochondria were suspended in swelling buffer and incubated for 1 h. The mitochondrial swelling was measured by following absorbance (λmax=540 nm) decrease.

Mentions: Induction of mitochondrial swelling in isolated lymphocyte mitochondria was monitored by following 540 nm absorbance (A540) decrease. EA addition (5, 10 and 20 µM) resulted in an extensive mitochondrial swelling in CLL mitochondria obtained from B lymphocytes of CLL patients (Fig. 9, graph B). EA addition to healthy mitochondria (5, 10 and 20 µM) not resulted mitochondrial swelling (Fig. 9, graph A). Furthermore, 5 µM cyclosporine A (an MPT blocker) strongly inhibited the decline of swelling in isolated B-lymphocytes mitochondria exposed to 10 μM of EA (p<0.05). An MPT inducer, CaCl2 (50 µM) was also used as a positive control in MMP assay (Fig. 9).


Ellagic acid, a polyphenolic compound, selectively induces ROS-mediated apoptosis in cancerous B-lymphocytes of CLL patients by directly targeting mitochondria.

Salimi A, Roudkenar MH, Sadeghi L, Mohseni A, Seydi E, Pirahmadi N, Pourahmad J - Redox Biol (2015)

Induction of mitochondrial swelling by EA. Addition of EA (5, 10 and 20 µM) induces mitochondrial swelling in CLL BUT NOT in healthy B lymphocyte mitochondria in a concentration depending manner. Mitochondria were suspended in swelling buffer and incubated for 1 h. The mitochondrial swelling was measured by following absorbance (λmax=540 nm) decrease.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588415&req=5

f0045: Induction of mitochondrial swelling by EA. Addition of EA (5, 10 and 20 µM) induces mitochondrial swelling in CLL BUT NOT in healthy B lymphocyte mitochondria in a concentration depending manner. Mitochondria were suspended in swelling buffer and incubated for 1 h. The mitochondrial swelling was measured by following absorbance (λmax=540 nm) decrease.
Mentions: Induction of mitochondrial swelling in isolated lymphocyte mitochondria was monitored by following 540 nm absorbance (A540) decrease. EA addition (5, 10 and 20 µM) resulted in an extensive mitochondrial swelling in CLL mitochondria obtained from B lymphocytes of CLL patients (Fig. 9, graph B). EA addition to healthy mitochondria (5, 10 and 20 µM) not resulted mitochondrial swelling (Fig. 9, graph A). Furthermore, 5 µM cyclosporine A (an MPT blocker) strongly inhibited the decline of swelling in isolated B-lymphocytes mitochondria exposed to 10 μM of EA (p<0.05). An MPT inducer, CaCl2 (50 µM) was also used as a positive control in MMP assay (Fig. 9).

Bottom Line: Based on our results EA decreased the percentage of viable cells and induced apoptosis.EA increased ROS formation, mitochondria swelling, MMP decrease and cytochrome c release in mitochondria isolated from CLL BUT NOT healthy B-lymphocytes while pre-treatment with cyclosporine A and Butylated hydroxyl toluene (BHT) prevented these effects.Our results suggest that EA can act as an anti cancer candidate by directly and selectively targeting mitochondria could induce apoptosis through mitochondria pathway with increasing ROS production which finally ends in cytochrome c release, caspase 3 activation and apoptosis in cancerous B-lymphocytes isolated from CLL patients.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

No MeSH data available.


Related in: MedlinePlus