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Ellagic acid, a polyphenolic compound, selectively induces ROS-mediated apoptosis in cancerous B-lymphocytes of CLL patients by directly targeting mitochondria.

Salimi A, Roudkenar MH, Sadeghi L, Mohseni A, Seydi E, Pirahmadi N, Pourahmad J - Redox Biol (2015)

Bottom Line: Based on our results EA decreased the percentage of viable cells and induced apoptosis.EA increased ROS formation, mitochondria swelling, MMP decrease and cytochrome c release in mitochondria isolated from CLL BUT NOT healthy B-lymphocytes while pre-treatment with cyclosporine A and Butylated hydroxyl toluene (BHT) prevented these effects.Our results suggest that EA can act as an anti cancer candidate by directly and selectively targeting mitochondria could induce apoptosis through mitochondria pathway with increasing ROS production which finally ends in cytochrome c release, caspase 3 activation and apoptosis in cancerous B-lymphocytes isolated from CLL patients.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

No MeSH data available.


Related in: MedlinePlus

Cell viability, B-lymphocytes from CLL and healthy donors, at 1×104 cells/well, were seeded on 96-well plates. EA at 20, 50 and 100 µM concentrations was incubated for 48 h. The absorbance representing the viability of B-lymphocytes was determined by the ELISA reader at 570 nm. Data presented as mean±SD. The significant level was p<0.001, n=5.
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f0005: Cell viability, B-lymphocytes from CLL and healthy donors, at 1×104 cells/well, were seeded on 96-well plates. EA at 20, 50 and 100 µM concentrations was incubated for 48 h. The absorbance representing the viability of B-lymphocytes was determined by the ELISA reader at 570 nm. Data presented as mean±SD. The significant level was p<0.001, n=5.

Mentions: For determination of anti-cancer effect of EA we used MTT assay. First we measured the probable cytotoxicity of EA on healthy B-lymphocytes. Our results on healthy B-lymphocytes showed that EA wascytotoxic at the highest concentration (200 mM), we therefore did not use this concentration when we decided to measure cytotoxicity of EA on CLL B-lymphocytes. Our results with MTT assayshowed that even at the lowest concentration (5 µM) EA has toxic effecttoward healthy B-lymphocytes. As shown in graph B, EA at concentrations of 20, 50 and 100 µM, significantly (p<0.001) reduced cell viability down to 59, 38 and 17 % respectively (Fig. 1, graph B), while no toxicitywas recordedat these concentrations on healthy B-lymphocytes (Fig. 1, graph A).


Ellagic acid, a polyphenolic compound, selectively induces ROS-mediated apoptosis in cancerous B-lymphocytes of CLL patients by directly targeting mitochondria.

Salimi A, Roudkenar MH, Sadeghi L, Mohseni A, Seydi E, Pirahmadi N, Pourahmad J - Redox Biol (2015)

Cell viability, B-lymphocytes from CLL and healthy donors, at 1×104 cells/well, were seeded on 96-well plates. EA at 20, 50 and 100 µM concentrations was incubated for 48 h. The absorbance representing the viability of B-lymphocytes was determined by the ELISA reader at 570 nm. Data presented as mean±SD. The significant level was p<0.001, n=5.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588415&req=5

f0005: Cell viability, B-lymphocytes from CLL and healthy donors, at 1×104 cells/well, were seeded on 96-well plates. EA at 20, 50 and 100 µM concentrations was incubated for 48 h. The absorbance representing the viability of B-lymphocytes was determined by the ELISA reader at 570 nm. Data presented as mean±SD. The significant level was p<0.001, n=5.
Mentions: For determination of anti-cancer effect of EA we used MTT assay. First we measured the probable cytotoxicity of EA on healthy B-lymphocytes. Our results on healthy B-lymphocytes showed that EA wascytotoxic at the highest concentration (200 mM), we therefore did not use this concentration when we decided to measure cytotoxicity of EA on CLL B-lymphocytes. Our results with MTT assayshowed that even at the lowest concentration (5 µM) EA has toxic effecttoward healthy B-lymphocytes. As shown in graph B, EA at concentrations of 20, 50 and 100 µM, significantly (p<0.001) reduced cell viability down to 59, 38 and 17 % respectively (Fig. 1, graph B), while no toxicitywas recordedat these concentrations on healthy B-lymphocytes (Fig. 1, graph A).

Bottom Line: Based on our results EA decreased the percentage of viable cells and induced apoptosis.EA increased ROS formation, mitochondria swelling, MMP decrease and cytochrome c release in mitochondria isolated from CLL BUT NOT healthy B-lymphocytes while pre-treatment with cyclosporine A and Butylated hydroxyl toluene (BHT) prevented these effects.Our results suggest that EA can act as an anti cancer candidate by directly and selectively targeting mitochondria could induce apoptosis through mitochondria pathway with increasing ROS production which finally ends in cytochrome c release, caspase 3 activation and apoptosis in cancerous B-lymphocytes isolated from CLL patients.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

No MeSH data available.


Related in: MedlinePlus