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Sodium selenite supplementation does not fully restore oxidative stress-induced deiodinase dysfunction: Implications for the nonthyroidal illness syndrome.

Wajner SM, Rohenkohl HC, Serrano T, Maia AL - Redox Biol (2015)

Bottom Line: Increased ROS was paralleled by D1 and D2-decreased T3-production (P<0.01) and increased D3-catalyzed T3-inactivation (P<0.001).Selenite decreases the IL6-induced ROS and carbonyl content, while enhances Gpx and Trx activities.In conclusion, although sodium selenite reduces IL6-induced redox imbalance it does not fully repair deiodinase function.

View Article: PubMed Central - PubMed

Affiliation: Thyroid Section, Endocrine Division, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

No MeSH data available.


Related in: MedlinePlus

Effect of sodium selenite or N-acetylcysteine (NAC) on glutathione peroxidase (GPx) and thioredoxin reductase (TRx) activity in controls and IL6 (1000 ng/L) treated cells. The column on the left shows the effect of sodium selenite or NAC on intracellular GSH levels in controls and IL6 (1000 ng/L) treated MSTO-211 (A), HepG2 (B), or MCF-7 (C) cells. The column on the right shows the effect of sodium selenite or NAC on intracellular TRx levels in MSTO-211 (D), HepG2 (E), or MCF-7 (F) cells. Data are mean±SD of at least three independent experiments.
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f0035: Effect of sodium selenite or N-acetylcysteine (NAC) on glutathione peroxidase (GPx) and thioredoxin reductase (TRx) activity in controls and IL6 (1000 ng/L) treated cells. The column on the left shows the effect of sodium selenite or NAC on intracellular GSH levels in controls and IL6 (1000 ng/L) treated MSTO-211 (A), HepG2 (B), or MCF-7 (C) cells. The column on the right shows the effect of sodium selenite or NAC on intracellular TRx levels in MSTO-211 (D), HepG2 (E), or MCF-7 (F) cells. Data are mean±SD of at least three independent experiments.

Mentions: Next we determined the extension of GPx and TRx consumption in the presence of IL6-induced oxidative stress and whether sodium selenite supplementation could rescue IL6 mediated effects. IL6 induced oxidative stress decreased both GPx and TRx activities in all cell lines whereas sodium selenite counteracted the IL6 effects (all P<0.05; Fig. 7A–F). NAC had no effect on GPx or TRx levels (all P>0.05; Fig. 7A–F).


Sodium selenite supplementation does not fully restore oxidative stress-induced deiodinase dysfunction: Implications for the nonthyroidal illness syndrome.

Wajner SM, Rohenkohl HC, Serrano T, Maia AL - Redox Biol (2015)

Effect of sodium selenite or N-acetylcysteine (NAC) on glutathione peroxidase (GPx) and thioredoxin reductase (TRx) activity in controls and IL6 (1000 ng/L) treated cells. The column on the left shows the effect of sodium selenite or NAC on intracellular GSH levels in controls and IL6 (1000 ng/L) treated MSTO-211 (A), HepG2 (B), or MCF-7 (C) cells. The column on the right shows the effect of sodium selenite or NAC on intracellular TRx levels in MSTO-211 (D), HepG2 (E), or MCF-7 (F) cells. Data are mean±SD of at least three independent experiments.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588414&req=5

f0035: Effect of sodium selenite or N-acetylcysteine (NAC) on glutathione peroxidase (GPx) and thioredoxin reductase (TRx) activity in controls and IL6 (1000 ng/L) treated cells. The column on the left shows the effect of sodium selenite or NAC on intracellular GSH levels in controls and IL6 (1000 ng/L) treated MSTO-211 (A), HepG2 (B), or MCF-7 (C) cells. The column on the right shows the effect of sodium selenite or NAC on intracellular TRx levels in MSTO-211 (D), HepG2 (E), or MCF-7 (F) cells. Data are mean±SD of at least three independent experiments.
Mentions: Next we determined the extension of GPx and TRx consumption in the presence of IL6-induced oxidative stress and whether sodium selenite supplementation could rescue IL6 mediated effects. IL6 induced oxidative stress decreased both GPx and TRx activities in all cell lines whereas sodium selenite counteracted the IL6 effects (all P<0.05; Fig. 7A–F). NAC had no effect on GPx or TRx levels (all P>0.05; Fig. 7A–F).

Bottom Line: Increased ROS was paralleled by D1 and D2-decreased T3-production (P<0.01) and increased D3-catalyzed T3-inactivation (P<0.001).Selenite decreases the IL6-induced ROS and carbonyl content, while enhances Gpx and Trx activities.In conclusion, although sodium selenite reduces IL6-induced redox imbalance it does not fully repair deiodinase function.

View Article: PubMed Central - PubMed

Affiliation: Thyroid Section, Endocrine Division, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

No MeSH data available.


Related in: MedlinePlus