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Sodium selenite supplementation does not fully restore oxidative stress-induced deiodinase dysfunction: Implications for the nonthyroidal illness syndrome.

Wajner SM, Rohenkohl HC, Serrano T, Maia AL - Redox Biol (2015)

Bottom Line: Increased ROS was paralleled by D1 and D2-decreased T3-production (P<0.01) and increased D3-catalyzed T3-inactivation (P<0.001).Selenite decreases the IL6-induced ROS and carbonyl content, while enhances Gpx and Trx activities.In conclusion, although sodium selenite reduces IL6-induced redox imbalance it does not fully repair deiodinase function.

View Article: PubMed Central - PubMed

Affiliation: Thyroid Section, Endocrine Division, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

No MeSH data available.


Related in: MedlinePlus

Effect of sodium selenite on type 3 deiodinase activity and expression. Sodium selenite partially reverses the effect of IL6 on deiodinase type 3 (D3) catalyzed T3 inactivation while NAC completely abolishes it (A). There was no effect on the IL6 induced DIO3 messenger RNA (mRNA) (B). *P<0.001, difference between control and IL6 treated cells; **P<0.001, difference between presence or not of sodium selenite; §P=0.01, difference between controls in the presence or absence of sodium elenite. Data are mean±SD of at least three independent experiments.
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f0025: Effect of sodium selenite on type 3 deiodinase activity and expression. Sodium selenite partially reverses the effect of IL6 on deiodinase type 3 (D3) catalyzed T3 inactivation while NAC completely abolishes it (A). There was no effect on the IL6 induced DIO3 messenger RNA (mRNA) (B). *P<0.001, difference between control and IL6 treated cells; **P<0.001, difference between presence or not of sodium selenite; §P=0.01, difference between controls in the presence or absence of sodium elenite. Data are mean±SD of at least three independent experiments.

Mentions: Next, we evaluated the effect of sodium selenite on D3 expression using MCF-7 cells [30]. The addition of IL6 induced D3-catalyzed T3 inactivation in a dose dependent fashion (P<0.001). In contrast to the results obtained for D2 and D1, the sodium selenite supplementation attenuated the IL6-induced increase in D3 activity (P<0.05; Fig. 5A). On the other hand, D3 activity return to the baseline levels with NAC addition. Either sodium selenite or NAC addition altered the IL6-induced up-regulation of DIO3 mRNA (Fig. 5B).


Sodium selenite supplementation does not fully restore oxidative stress-induced deiodinase dysfunction: Implications for the nonthyroidal illness syndrome.

Wajner SM, Rohenkohl HC, Serrano T, Maia AL - Redox Biol (2015)

Effect of sodium selenite on type 3 deiodinase activity and expression. Sodium selenite partially reverses the effect of IL6 on deiodinase type 3 (D3) catalyzed T3 inactivation while NAC completely abolishes it (A). There was no effect on the IL6 induced DIO3 messenger RNA (mRNA) (B). *P<0.001, difference between control and IL6 treated cells; **P<0.001, difference between presence or not of sodium selenite; §P=0.01, difference between controls in the presence or absence of sodium elenite. Data are mean±SD of at least three independent experiments.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588414&req=5

f0025: Effect of sodium selenite on type 3 deiodinase activity and expression. Sodium selenite partially reverses the effect of IL6 on deiodinase type 3 (D3) catalyzed T3 inactivation while NAC completely abolishes it (A). There was no effect on the IL6 induced DIO3 messenger RNA (mRNA) (B). *P<0.001, difference between control and IL6 treated cells; **P<0.001, difference between presence or not of sodium selenite; §P=0.01, difference between controls in the presence or absence of sodium elenite. Data are mean±SD of at least three independent experiments.
Mentions: Next, we evaluated the effect of sodium selenite on D3 expression using MCF-7 cells [30]. The addition of IL6 induced D3-catalyzed T3 inactivation in a dose dependent fashion (P<0.001). In contrast to the results obtained for D2 and D1, the sodium selenite supplementation attenuated the IL6-induced increase in D3 activity (P<0.05; Fig. 5A). On the other hand, D3 activity return to the baseline levels with NAC addition. Either sodium selenite or NAC addition altered the IL6-induced up-regulation of DIO3 mRNA (Fig. 5B).

Bottom Line: Increased ROS was paralleled by D1 and D2-decreased T3-production (P<0.01) and increased D3-catalyzed T3-inactivation (P<0.001).Selenite decreases the IL6-induced ROS and carbonyl content, while enhances Gpx and Trx activities.In conclusion, although sodium selenite reduces IL6-induced redox imbalance it does not fully repair deiodinase function.

View Article: PubMed Central - PubMed

Affiliation: Thyroid Section, Endocrine Division, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

No MeSH data available.


Related in: MedlinePlus