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Breast Cancer Cell Line Aggregate Morphology Does Not Predict Invasive Capacity.

Ziperstein MJ, Guzman A, Kaufman LJ - PLoS ONE (2015)

Bottom Line: To invade and metastasize to distant loci, breast cancer cells must breach the layer of basement membrane surrounding the tumor and then invade through the dense collagen I-rich extracellular environment of breast tissue.Moreover, cell lines from the same aggregate morphological class exhibited similarities in gene expression patterns.Correlations between the 3D spheroid invasion assay and gene expression profiles suggest this assay as an inexpensive functional method to predict breast cancer invasive capacity.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Columbia University, New York, New York, United States of America.

ABSTRACT
To invade and metastasize to distant loci, breast cancer cells must breach the layer of basement membrane surrounding the tumor and then invade through the dense collagen I-rich extracellular environment of breast tissue. Previous studies have shown that breast cancer cell aggregate morphology in basement membrane extract correlated with cell invasive capacity in some contexts. Moreover, cell lines from the same aggregate morphological class exhibited similarities in gene expression patterns. To further assess the capacity of cell and aggregate morphology to predict invasive capacity in physiologically relevant environments, six cell lines with varied cell aggregate morphologies were assessed in a variety of assays including a 3D multicellular invasion assay that recapitulates cell-cell and cell-environment contacts as they exist in vivo in the context of the primary breast tumor. Migratory and invasive capacities as measured through a 2D gap assay and a 3D spheroid invasion assay reveal that breast cancer cell aggregate morphology alone is insufficient to predict migratory speed in 2D or invasive capacity in 3D. Correlations between the 3D spheroid invasion assay and gene expression profiles suggest this assay as an inexpensive functional method to predict breast cancer invasive capacity.

No MeSH data available.


Related in: MedlinePlus

Transmittance images of cells from each cell line investigated in a gap assay at 24 hours.Initial gap distance is 500 μm, the height of the images. Scale bar is 200 μm.
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pone.0139523.g002: Transmittance images of cells from each cell line investigated in a gap assay at 24 hours.Initial gap distance is 500 μm, the height of the images. Scale bar is 200 μm.

Mentions: Among cells with stellate aggregate morphology in 3D collagen I and on 2D tissue culture treated glass, the MDA-MB-231 cells migrated most rapidly. After 6 hours, the cell front of the MDA-MB-231 monolayers had closed substantially, with select cells having reached the center of the gap, and by 24 hours, the gap was completely filled in (Fig 2). The Hs 578T and MDA-MB-157 cells also migrated individually, though more slowly than the MDA-MB-231 cells. Some cells had spanned the gap by 24 hours but the gap was not completely filled in by that time point.


Breast Cancer Cell Line Aggregate Morphology Does Not Predict Invasive Capacity.

Ziperstein MJ, Guzman A, Kaufman LJ - PLoS ONE (2015)

Transmittance images of cells from each cell line investigated in a gap assay at 24 hours.Initial gap distance is 500 μm, the height of the images. Scale bar is 200 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4587946&req=5

pone.0139523.g002: Transmittance images of cells from each cell line investigated in a gap assay at 24 hours.Initial gap distance is 500 μm, the height of the images. Scale bar is 200 μm.
Mentions: Among cells with stellate aggregate morphology in 3D collagen I and on 2D tissue culture treated glass, the MDA-MB-231 cells migrated most rapidly. After 6 hours, the cell front of the MDA-MB-231 monolayers had closed substantially, with select cells having reached the center of the gap, and by 24 hours, the gap was completely filled in (Fig 2). The Hs 578T and MDA-MB-157 cells also migrated individually, though more slowly than the MDA-MB-231 cells. Some cells had spanned the gap by 24 hours but the gap was not completely filled in by that time point.

Bottom Line: To invade and metastasize to distant loci, breast cancer cells must breach the layer of basement membrane surrounding the tumor and then invade through the dense collagen I-rich extracellular environment of breast tissue.Moreover, cell lines from the same aggregate morphological class exhibited similarities in gene expression patterns.Correlations between the 3D spheroid invasion assay and gene expression profiles suggest this assay as an inexpensive functional method to predict breast cancer invasive capacity.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Columbia University, New York, New York, United States of America.

ABSTRACT
To invade and metastasize to distant loci, breast cancer cells must breach the layer of basement membrane surrounding the tumor and then invade through the dense collagen I-rich extracellular environment of breast tissue. Previous studies have shown that breast cancer cell aggregate morphology in basement membrane extract correlated with cell invasive capacity in some contexts. Moreover, cell lines from the same aggregate morphological class exhibited similarities in gene expression patterns. To further assess the capacity of cell and aggregate morphology to predict invasive capacity in physiologically relevant environments, six cell lines with varied cell aggregate morphologies were assessed in a variety of assays including a 3D multicellular invasion assay that recapitulates cell-cell and cell-environment contacts as they exist in vivo in the context of the primary breast tumor. Migratory and invasive capacities as measured through a 2D gap assay and a 3D spheroid invasion assay reveal that breast cancer cell aggregate morphology alone is insufficient to predict migratory speed in 2D or invasive capacity in 3D. Correlations between the 3D spheroid invasion assay and gene expression profiles suggest this assay as an inexpensive functional method to predict breast cancer invasive capacity.

No MeSH data available.


Related in: MedlinePlus