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Global comparison of chromosome X genes of pulmonary telocytes with mesenchymal stem cells, fibroblasts, alveolar type II cells, airway epithelial cells, and lymphocytes.

Zhu Y, Zheng M, Song D, Ye L, Wang X - J Transl Med (2015)

Bottom Line: We compared gene expression profiles in chromosome X of pulmonary TCs with mesenchymal stem cells (MSC), fibroblasts (Fb), alveolar type II cells (ATII), airway basal cells (ABC), proximal airway cells (PAC), CD8(+) T cells come from bronchial lymph nodes (T-BL), or CD8(+) T cells from lungs (T-L) by global analyses, and selected the genes which were consistently up or down regulated (>1 fold) in TCs compared to other cells as TC-specific genes.The functional and characteristic networks were identified and compared by bioinformatics tools.According to the selected TC-specific genes, we infer that pulmonary TCs function as modulators which may enhance cellular growth and migration, resist senescence, protect cells from external stress, regulate immune responses, participate in tissue remodeling and repair, regulate neural function, and promote vessel formation.

View Article: PubMed Central - PubMed

Affiliation: Zhongshan Hospital, Shanghai Institute of Clinical Bioinformatics, Fudan University Center for Bioinformatics, Fudan University, Shanghai, China. zhu.yichun@zs-hospital.com.

ABSTRACT

Background: Telocytes (TCs) are suggested as a new type of interstitial cells with specific telopodes. Our previous study evidenced that TCs differed from fibroblasts and stem cells at the aspect of gene expression profiles. The present study aims to search the characters and patterns of chromosome X genes of TC-specific or TC-dominated gene profiles and fingerprints, investigate the network of principle genes, and explore potential functional association.

Methods: We compared gene expression profiles in chromosome X of pulmonary TCs with mesenchymal stem cells (MSC), fibroblasts (Fb), alveolar type II cells (ATII), airway basal cells (ABC), proximal airway cells (PAC), CD8(+) T cells come from bronchial lymph nodes (T-BL), or CD8(+) T cells from lungs (T-L) by global analyses, and selected the genes which were consistently up or down regulated (>1 fold) in TCs compared to other cells as TC-specific genes. The functional and characteristic networks were identified and compared by bioinformatics tools.

Results: We selected 31 chromosome X genes as the TC-specific or dominated genes, among which 8 up-regulated (Flna, Msn, Cfp, Col4a5, Mum1l1, Rnf128, Syn1, and Srpx2) and 23 down-regulated (Abcb7, Atf1, Ddx26b, Drp2, Fam122b, Gyk, Irak1, Lamp2, Mecp2, Ndufb11, Ogt, Pdha1, Pola1, Rab9, Rbmx2, Rhox9, Thoc2, Vbp1, Dkc1, Nkrf, Piga, Tmlhe and Tsr2), as compared with other cells.

Conclusions: Our data suggested that gene expressions of chromosome X in TCs are different with those in other cells in the lung tissue. According to the selected TC-specific genes, we infer that pulmonary TCs function as modulators which may enhance cellular growth and migration, resist senescence, protect cells from external stress, regulate immune responses, participate in tissue remodeling and repair, regulate neural function, and promote vessel formation.

No MeSH data available.


Related in: MedlinePlus

TC-specific or dominant genes and their main function. Gene expression array data showed that 8 chromosome X genes up-regulated in both TC5 and TC10, and 23 genes down-regulated, as compared with those in other cells. These genes are selected as TC-dominant or specific genes. The up-regulated genes showed in red color while the down-regulated genes in green color. The up-regulated genes are mostly involved in cytoskeleton and cell migration, and regulation of immune responses. The down-regulated genes participates mainly in gene transcription modulation, energy metastasis, material transportation and so on
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Fig4: TC-specific or dominant genes and their main function. Gene expression array data showed that 8 chromosome X genes up-regulated in both TC5 and TC10, and 23 genes down-regulated, as compared with those in other cells. These genes are selected as TC-dominant or specific genes. The up-regulated genes showed in red color while the down-regulated genes in green color. The up-regulated genes are mostly involved in cytoskeleton and cell migration, and regulation of immune responses. The down-regulated genes participates mainly in gene transcription modulation, energy metastasis, material transportation and so on

Mentions: Chromosome X is one of the two sex-determining chromosomes, which exists in both gender, with one copy in male and two copies in female, spanning about 156 million base pairs and 1805 genes in human cells. Although chromosome X contains only 4 % of all human genes, a large number of disease condition are related with chromosome X, including X-linked diseases and 10 % of diseases with a mendelian pattern of inheritance [39]. There are 20,000–25,000 genes in mouse, and the similarity of genes between human and mouse is about 85 %. Among 2059 genes in chromosome X of the mouse, 335 were measured by bioinformatics tools in the present study. There were about 8 or 23 up- or down-regulated genes of chromosome X in TCs, as compared with stem cells, lung interstitial cells, pneumocytes, airway cells, or lymphocytes (Fig. 4).Fig. 4


Global comparison of chromosome X genes of pulmonary telocytes with mesenchymal stem cells, fibroblasts, alveolar type II cells, airway epithelial cells, and lymphocytes.

Zhu Y, Zheng M, Song D, Ye L, Wang X - J Transl Med (2015)

TC-specific or dominant genes and their main function. Gene expression array data showed that 8 chromosome X genes up-regulated in both TC5 and TC10, and 23 genes down-regulated, as compared with those in other cells. These genes are selected as TC-dominant or specific genes. The up-regulated genes showed in red color while the down-regulated genes in green color. The up-regulated genes are mostly involved in cytoskeleton and cell migration, and regulation of immune responses. The down-regulated genes participates mainly in gene transcription modulation, energy metastasis, material transportation and so on
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4587873&req=5

Fig4: TC-specific or dominant genes and their main function. Gene expression array data showed that 8 chromosome X genes up-regulated in both TC5 and TC10, and 23 genes down-regulated, as compared with those in other cells. These genes are selected as TC-dominant or specific genes. The up-regulated genes showed in red color while the down-regulated genes in green color. The up-regulated genes are mostly involved in cytoskeleton and cell migration, and regulation of immune responses. The down-regulated genes participates mainly in gene transcription modulation, energy metastasis, material transportation and so on
Mentions: Chromosome X is one of the two sex-determining chromosomes, which exists in both gender, with one copy in male and two copies in female, spanning about 156 million base pairs and 1805 genes in human cells. Although chromosome X contains only 4 % of all human genes, a large number of disease condition are related with chromosome X, including X-linked diseases and 10 % of diseases with a mendelian pattern of inheritance [39]. There are 20,000–25,000 genes in mouse, and the similarity of genes between human and mouse is about 85 %. Among 2059 genes in chromosome X of the mouse, 335 were measured by bioinformatics tools in the present study. There were about 8 or 23 up- or down-regulated genes of chromosome X in TCs, as compared with stem cells, lung interstitial cells, pneumocytes, airway cells, or lymphocytes (Fig. 4).Fig. 4

Bottom Line: We compared gene expression profiles in chromosome X of pulmonary TCs with mesenchymal stem cells (MSC), fibroblasts (Fb), alveolar type II cells (ATII), airway basal cells (ABC), proximal airway cells (PAC), CD8(+) T cells come from bronchial lymph nodes (T-BL), or CD8(+) T cells from lungs (T-L) by global analyses, and selected the genes which were consistently up or down regulated (>1 fold) in TCs compared to other cells as TC-specific genes.The functional and characteristic networks were identified and compared by bioinformatics tools.According to the selected TC-specific genes, we infer that pulmonary TCs function as modulators which may enhance cellular growth and migration, resist senescence, protect cells from external stress, regulate immune responses, participate in tissue remodeling and repair, regulate neural function, and promote vessel formation.

View Article: PubMed Central - PubMed

Affiliation: Zhongshan Hospital, Shanghai Institute of Clinical Bioinformatics, Fudan University Center for Bioinformatics, Fudan University, Shanghai, China. zhu.yichun@zs-hospital.com.

ABSTRACT

Background: Telocytes (TCs) are suggested as a new type of interstitial cells with specific telopodes. Our previous study evidenced that TCs differed from fibroblasts and stem cells at the aspect of gene expression profiles. The present study aims to search the characters and patterns of chromosome X genes of TC-specific or TC-dominated gene profiles and fingerprints, investigate the network of principle genes, and explore potential functional association.

Methods: We compared gene expression profiles in chromosome X of pulmonary TCs with mesenchymal stem cells (MSC), fibroblasts (Fb), alveolar type II cells (ATII), airway basal cells (ABC), proximal airway cells (PAC), CD8(+) T cells come from bronchial lymph nodes (T-BL), or CD8(+) T cells from lungs (T-L) by global analyses, and selected the genes which were consistently up or down regulated (>1 fold) in TCs compared to other cells as TC-specific genes. The functional and characteristic networks were identified and compared by bioinformatics tools.

Results: We selected 31 chromosome X genes as the TC-specific or dominated genes, among which 8 up-regulated (Flna, Msn, Cfp, Col4a5, Mum1l1, Rnf128, Syn1, and Srpx2) and 23 down-regulated (Abcb7, Atf1, Ddx26b, Drp2, Fam122b, Gyk, Irak1, Lamp2, Mecp2, Ndufb11, Ogt, Pdha1, Pola1, Rab9, Rbmx2, Rhox9, Thoc2, Vbp1, Dkc1, Nkrf, Piga, Tmlhe and Tsr2), as compared with other cells.

Conclusions: Our data suggested that gene expressions of chromosome X in TCs are different with those in other cells in the lung tissue. According to the selected TC-specific genes, we infer that pulmonary TCs function as modulators which may enhance cellular growth and migration, resist senescence, protect cells from external stress, regulate immune responses, participate in tissue remodeling and repair, regulate neural function, and promote vessel formation.

No MeSH data available.


Related in: MedlinePlus