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Expression of pluripotency markers in the bovine uterus with adenomyosis.

Łupicka M, Socha B, Szczepańska A, Korzekwa A - Reprod. Biol. Endocrinol. (2015)

Bottom Line: Adenomyosis is a proliferative uterine dysfunction with unknown aetiology.One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue.Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. m.lupicka@pan.olsztyn.pl.

ABSTRACT

Background: Adenomyosis is a proliferative uterine dysfunction with unknown aetiology. One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue. Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.

Findings: Immunolocalisation revealed protein expression of NANOG, OCT4 and SOX2 in both normal and adenomyotic uteri. mRNA expression for NANOG and OCT4 was increased in tissues obtained from uteri with adenomyosis compared to controls, but at the protein level there were no significant differences. mRNA expression for all three pluripotency markers was higher in myometrial cells isolated from uteri with adenomyotic lesions than in those isolated from normal uteri. The protein level of NANOG and SOX2 was decreased in stromal cells from adenomyotic tissues, whereas the level of OCT4 and SOX2 was increased in myometrial cells obtained from dysfunctional uteri.

Conclusions: The results indicate significant changes in expression of pluripotency markers in adenomyotic compared to normal uteri, which suggest the involvement of uterine stem cells in adenomyosis.

No MeSH data available.


Related in: MedlinePlus

Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. NANOG a OCT4 b and SOX2 c protein expression in uterine stromal cells. NANOG d OCT4 e and SOX2 f protein expression in uterine myometrial cells. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical differences between uterine normal and adenomyotic tissue (*P < 0.05; **P < 0.01), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs g. MM – molecular weight marker, C – cells obtained from control cows, ADENO – cells obtained from cows with adenomyosis
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Fig8: Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. NANOG a OCT4 b and SOX2 c protein expression in uterine stromal cells. NANOG d OCT4 e and SOX2 f protein expression in uterine myometrial cells. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical differences between uterine normal and adenomyotic tissue (*P < 0.05; **P < 0.01), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs g. MM – molecular weight marker, C – cells obtained from control cows, ADENO – cells obtained from cows with adenomyosis

Mentions: Protein expression of NANOG and SOX2 was significantly decreased in stromal cells isolated from uteri with adenomyosis compared to those obtained from normal uteri (P < 0.05, Fig. 8a, c). Protein expression of both transcription factors OCT4 and SOX2 was higher in cultured myometrial cells from adenomyotic tissues than in corresponding cells isolated from normal uteri (P < 0.05, Fig. 8e, f).Fig. 8


Expression of pluripotency markers in the bovine uterus with adenomyosis.

Łupicka M, Socha B, Szczepańska A, Korzekwa A - Reprod. Biol. Endocrinol. (2015)

Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. NANOG a OCT4 b and SOX2 c protein expression in uterine stromal cells. NANOG d OCT4 e and SOX2 f protein expression in uterine myometrial cells. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical differences between uterine normal and adenomyotic tissue (*P < 0.05; **P < 0.01), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs g. MM – molecular weight marker, C – cells obtained from control cows, ADENO – cells obtained from cows with adenomyosis
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Related In: Results  -  Collection

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Fig8: Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. NANOG a OCT4 b and SOX2 c protein expression in uterine stromal cells. NANOG d OCT4 e and SOX2 f protein expression in uterine myometrial cells. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical differences between uterine normal and adenomyotic tissue (*P < 0.05; **P < 0.01), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs g. MM – molecular weight marker, C – cells obtained from control cows, ADENO – cells obtained from cows with adenomyosis
Mentions: Protein expression of NANOG and SOX2 was significantly decreased in stromal cells isolated from uteri with adenomyosis compared to those obtained from normal uteri (P < 0.05, Fig. 8a, c). Protein expression of both transcription factors OCT4 and SOX2 was higher in cultured myometrial cells from adenomyotic tissues than in corresponding cells isolated from normal uteri (P < 0.05, Fig. 8e, f).Fig. 8

Bottom Line: Adenomyosis is a proliferative uterine dysfunction with unknown aetiology.One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue.Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. m.lupicka@pan.olsztyn.pl.

ABSTRACT

Background: Adenomyosis is a proliferative uterine dysfunction with unknown aetiology. One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue. Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.

Findings: Immunolocalisation revealed protein expression of NANOG, OCT4 and SOX2 in both normal and adenomyotic uteri. mRNA expression for NANOG and OCT4 was increased in tissues obtained from uteri with adenomyosis compared to controls, but at the protein level there were no significant differences. mRNA expression for all three pluripotency markers was higher in myometrial cells isolated from uteri with adenomyotic lesions than in those isolated from normal uteri. The protein level of NANOG and SOX2 was decreased in stromal cells from adenomyotic tissues, whereas the level of OCT4 and SOX2 was increased in myometrial cells obtained from dysfunctional uteri.

Conclusions: The results indicate significant changes in expression of pluripotency markers in adenomyotic compared to normal uteri, which suggest the involvement of uterine stem cells in adenomyosis.

No MeSH data available.


Related in: MedlinePlus