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Expression of pluripotency markers in the bovine uterus with adenomyosis.

Łupicka M, Socha B, Szczepańska A, Korzekwa A - Reprod. Biol. Endocrinol. (2015)

Bottom Line: Adenomyosis is a proliferative uterine dysfunction with unknown aetiology.One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue.Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. m.lupicka@pan.olsztyn.pl.

ABSTRACT

Background: Adenomyosis is a proliferative uterine dysfunction with unknown aetiology. One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue. Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.

Findings: Immunolocalisation revealed protein expression of NANOG, OCT4 and SOX2 in both normal and adenomyotic uteri. mRNA expression for NANOG and OCT4 was increased in tissues obtained from uteri with adenomyosis compared to controls, but at the protein level there were no significant differences. mRNA expression for all three pluripotency markers was higher in myometrial cells isolated from uteri with adenomyotic lesions than in those isolated from normal uteri. The protein level of NANOG and SOX2 was decreased in stromal cells from adenomyotic tissues, whereas the level of OCT4 and SOX2 was increased in myometrial cells obtained from dysfunctional uteri.

Conclusions: The results indicate significant changes in expression of pluripotency markers in adenomyotic compared to normal uteri, which suggest the involvement of uterine stem cells in adenomyosis.

No MeSH data available.


Related in: MedlinePlus

Protein expression of NANOG a, OCT4 b and SOX2 c in bovine uterine tissues obtained from control cows and from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. There were no statistical differences between uterine normal and adenomyotic tissues (P > 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis
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Fig6: Protein expression of NANOG a, OCT4 b and SOX2 c in bovine uterine tissues obtained from control cows and from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. There were no statistical differences between uterine normal and adenomyotic tissues (P > 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis

Mentions: At the protein expression level, determined by Western blotting, there were no significant differences among NANOG, OCT4 and SOX2 (P > 0.05, Fig. 6a–c). However the spatial differences in examined proteins expression was reported during IHC assay.Fig. 6


Expression of pluripotency markers in the bovine uterus with adenomyosis.

Łupicka M, Socha B, Szczepańska A, Korzekwa A - Reprod. Biol. Endocrinol. (2015)

Protein expression of NANOG a, OCT4 b and SOX2 c in bovine uterine tissues obtained from control cows and from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. There were no statistical differences between uterine normal and adenomyotic tissues (P > 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4587725&req=5

Fig6: Protein expression of NANOG a, OCT4 b and SOX2 c in bovine uterine tissues obtained from control cows and from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. There were no statistical differences between uterine normal and adenomyotic tissues (P > 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis
Mentions: At the protein expression level, determined by Western blotting, there were no significant differences among NANOG, OCT4 and SOX2 (P > 0.05, Fig. 6a–c). However the spatial differences in examined proteins expression was reported during IHC assay.Fig. 6

Bottom Line: Adenomyosis is a proliferative uterine dysfunction with unknown aetiology.One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue.Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. m.lupicka@pan.olsztyn.pl.

ABSTRACT

Background: Adenomyosis is a proliferative uterine dysfunction with unknown aetiology. One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue. Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.

Findings: Immunolocalisation revealed protein expression of NANOG, OCT4 and SOX2 in both normal and adenomyotic uteri. mRNA expression for NANOG and OCT4 was increased in tissues obtained from uteri with adenomyosis compared to controls, but at the protein level there were no significant differences. mRNA expression for all three pluripotency markers was higher in myometrial cells isolated from uteri with adenomyotic lesions than in those isolated from normal uteri. The protein level of NANOG and SOX2 was decreased in stromal cells from adenomyotic tissues, whereas the level of OCT4 and SOX2 was increased in myometrial cells obtained from dysfunctional uteri.

Conclusions: The results indicate significant changes in expression of pluripotency markers in adenomyotic compared to normal uteri, which suggest the involvement of uterine stem cells in adenomyosis.

No MeSH data available.


Related in: MedlinePlus