Limits...
Phytoremedial effect of Withania somnifera against arsenic-induced testicular toxicity in Charles Foster rats.

Kumar A, Kumar R, Rahman MS, Iqubal MA, Anand G, Niraj PK, Ali M - Avicenna J Phytomed (2015 Jul-Aug)

Bottom Line: The study revealed that after administration of sodium arsenite, there was a decrease in the sperm counts and sperm motility accompanied by an increased incidence of sperm abnormalities and hormonal imbalance leading to infertility.The study concludes that Withania somnifera possesses phytoremedial effect.It is one of the best antidotes against arsenic-induced reproductive toxicity.

View Article: PubMed Central - PubMed

Affiliation: Mahavir Cancer Institute & Research Centre, Patna, Bihar, India.

ABSTRACT

Objective: The main objective of the current study was to observe the ameliorative effect of Withania somnifera on arsenic-induced testicular toxicity by exploring the crucial parameters such as sperm counts, sperm motility, hormonal assay and lipid peroxidation including histopathology.

Materials and methods: In the present study, arsenic in the form of sodium arsenite was administered orally to male Charles Foster rats for 45 days. Thereafter, ethanolic root extract of Withania somnifera was administered for 30 days to observe its ameliorative effect on male reproductive system.

Results: The study revealed that after administration of sodium arsenite, there was a decrease in the sperm counts and sperm motility accompanied by an increased incidence of sperm abnormalities and hormonal imbalance leading to infertility. However, after administration of Withania somnifera, there was significant reversal in the parameters denoting that it not only possesses antioxidant and rejuvenating property but also maintains the cellular integrity of testicular cells leading to normal functioning of it.

Conclusion: The study concludes that Withania somnifera possesses phytoremedial effect. It is one of the best antidotes against arsenic-induced reproductive toxicity.

No MeSH data available.


Related in: MedlinePlus

Light microphotograph (H&E stained) section of sodium arsenite treated testicular section showing severe degeneration. Seminiferous tubules with no spermatogenetic stages or if are present, only 5% denotes the normal functioning. The leydig cells are in highly degenerative condition as hemorrhage in them is observed x400.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4587609&req=5

Figure 7: Light microphotograph (H&E stained) section of sodium arsenite treated testicular section showing severe degeneration. Seminiferous tubules with no spermatogenetic stages or if are present, only 5% denotes the normal functioning. The leydig cells are in highly degenerative condition as hemorrhage in them is observed x400.

Mentions: However, in sodium arsenite-treated testicular sections, it caused severe damage to the testicular cells. The seminiferous tubules showed only 5% of the functioning of the testicular cells. The leydig cells, also appeared to be in highly degenerative condition as hemorrhage was observed within them (Figures.7 and 8).


Phytoremedial effect of Withania somnifera against arsenic-induced testicular toxicity in Charles Foster rats.

Kumar A, Kumar R, Rahman MS, Iqubal MA, Anand G, Niraj PK, Ali M - Avicenna J Phytomed (2015 Jul-Aug)

Light microphotograph (H&E stained) section of sodium arsenite treated testicular section showing severe degeneration. Seminiferous tubules with no spermatogenetic stages or if are present, only 5% denotes the normal functioning. The leydig cells are in highly degenerative condition as hemorrhage in them is observed x400.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4587609&req=5

Figure 7: Light microphotograph (H&E stained) section of sodium arsenite treated testicular section showing severe degeneration. Seminiferous tubules with no spermatogenetic stages or if are present, only 5% denotes the normal functioning. The leydig cells are in highly degenerative condition as hemorrhage in them is observed x400.
Mentions: However, in sodium arsenite-treated testicular sections, it caused severe damage to the testicular cells. The seminiferous tubules showed only 5% of the functioning of the testicular cells. The leydig cells, also appeared to be in highly degenerative condition as hemorrhage was observed within them (Figures.7 and 8).

Bottom Line: The study revealed that after administration of sodium arsenite, there was a decrease in the sperm counts and sperm motility accompanied by an increased incidence of sperm abnormalities and hormonal imbalance leading to infertility.The study concludes that Withania somnifera possesses phytoremedial effect.It is one of the best antidotes against arsenic-induced reproductive toxicity.

View Article: PubMed Central - PubMed

Affiliation: Mahavir Cancer Institute & Research Centre, Patna, Bihar, India.

ABSTRACT

Objective: The main objective of the current study was to observe the ameliorative effect of Withania somnifera on arsenic-induced testicular toxicity by exploring the crucial parameters such as sperm counts, sperm motility, hormonal assay and lipid peroxidation including histopathology.

Materials and methods: In the present study, arsenic in the form of sodium arsenite was administered orally to male Charles Foster rats for 45 days. Thereafter, ethanolic root extract of Withania somnifera was administered for 30 days to observe its ameliorative effect on male reproductive system.

Results: The study revealed that after administration of sodium arsenite, there was a decrease in the sperm counts and sperm motility accompanied by an increased incidence of sperm abnormalities and hormonal imbalance leading to infertility. However, after administration of Withania somnifera, there was significant reversal in the parameters denoting that it not only possesses antioxidant and rejuvenating property but also maintains the cellular integrity of testicular cells leading to normal functioning of it.

Conclusion: The study concludes that Withania somnifera possesses phytoremedial effect. It is one of the best antidotes against arsenic-induced reproductive toxicity.

No MeSH data available.


Related in: MedlinePlus