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Antioxidant and cytotoxic activities of three species of tropical seaweeds.

Chia YY, Kanthimathi MS, Khoo KS, Rajarajeswaran J, Cheng HM, Yap WS - BMC Complement Altern Med (2015)

Bottom Line: The activity of the cellular antioxidant enzymes, superoxide dismutase, catalase and glutathione reductase, in MCF-7 cells, decreased in a time-dependent manner.LC-MS analysis revealed the presence of bioactive alkaloids such as camptothecin, lycodine and pesudopelletierine.Based on the results obtained, all three seaweeds are rich sources of enzymatic and non-enzymatic antioxidants which could contribute to their reported medicinal benefits.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. gracechia1986@hotmail.com.

ABSTRACT

Background: Three species of seaweeds (Padina tetrastromatica, Caulerpa racemosa and Turbinaria ornata) are widely consumed by Asians as nutraceutical food due to their antioxidant properties. Studies have shown that these seaweeds exhibit bioactivities which include antimicrobial, antiviral, anti-hypertensive and anticoagulant activities. However, investigations into the mechanisms of action pertaining to the cytotoxic activity of the seaweeds are limited. The aim of this study was to determine the antioxidant and cytotoxic activities of whole extracts of P. tetrastromatica, C. racemosa and T. ornata, including the cellular events leading to the apoptotic cell death of the extract treated-MCF-7 cells. Bioassay guided fractionation was carried out and the compounds identified.

Methods: Powdered samples were sequentially extracted for 24 h. Their antioxidant activities were assessed by the DPPH radical, superoxide, nitric oxide and hydroxyl radical scavenging assays. The cytotoxic activity of the extract-treated MCF-7cells was assessed using the MTT assay. The most potent fraction was subjected to bioassay guided fractionation with column chromatography. All the fractions were tested for cytotoxic activity, caspase activity and effect on DNA fragmentation.

Results: All three seaweeds showed potent radical scavenging activities in the various assays. The activity of the cellular antioxidant enzymes, superoxide dismutase, catalase and glutathione reductase, in MCF-7 cells, decreased in a time-dependent manner. The partially purified fractions exhibited higher cytotoxic activity, as assessed by the MTT assay, than the whole extracts in the breast adenocarcinoma cell line, MCF-7. LC-MS analysis revealed the presence of bioactive alkaloids such as camptothecin, lycodine and pesudopelletierine.

Conclusion: Based on the results obtained, all three seaweeds are rich sources of enzymatic and non-enzymatic antioxidants which could contribute to their reported medicinal benefits.

No MeSH data available.


Related in: MedlinePlus

MS spectra of protonated and deprotonated molecules. a Camptothecin at [M + H] + of m/z 371; lycodine at [M-H]- of m/z 277; camptothecin at [M + H] + of m/z 371; pseudopelletierine at [M + H] + of m/z 171; Camptothecin at [M + H] + of m/z 371; Pseudopelletierine at [M + H] + of m/z 171; Camptothecin at [M + H] + of m/z 371. b Compounds with pharmacological activity isolated and identified in P. tetrastromatica, C. racemosa and T. ornata fractions
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Fig2: MS spectra of protonated and deprotonated molecules. a Camptothecin at [M + H] + of m/z 371; lycodine at [M-H]- of m/z 277; camptothecin at [M + H] + of m/z 371; pseudopelletierine at [M + H] + of m/z 171; Camptothecin at [M + H] + of m/z 371; Pseudopelletierine at [M + H] + of m/z 171; Camptothecin at [M + H] + of m/z 371. b Compounds with pharmacological activity isolated and identified in P. tetrastromatica, C. racemosa and T. ornata fractions

Mentions: Following determination of cytotoxic activity, the pooled fractions P2 of P. tetrastromatica, P1 of C. racemosa and P1 of T. ornata were subjected to LC-MS analysis. Analysis of the mass spectral data for peaks at m/z 371 revealed the presence of camptothecin eluted at 26.5 min. Pseudopelletierine eluted at 12.7 min and lycodine at 20.5 min, showed fragments at m/z 171 and 277, respectively (Fig. 2a). The compounds detected are summarised in Fig. 2b.Fig. 2


Antioxidant and cytotoxic activities of three species of tropical seaweeds.

Chia YY, Kanthimathi MS, Khoo KS, Rajarajeswaran J, Cheng HM, Yap WS - BMC Complement Altern Med (2015)

MS spectra of protonated and deprotonated molecules. a Camptothecin at [M + H] + of m/z 371; lycodine at [M-H]- of m/z 277; camptothecin at [M + H] + of m/z 371; pseudopelletierine at [M + H] + of m/z 171; Camptothecin at [M + H] + of m/z 371; Pseudopelletierine at [M + H] + of m/z 171; Camptothecin at [M + H] + of m/z 371. b Compounds with pharmacological activity isolated and identified in P. tetrastromatica, C. racemosa and T. ornata fractions
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4587585&req=5

Fig2: MS spectra of protonated and deprotonated molecules. a Camptothecin at [M + H] + of m/z 371; lycodine at [M-H]- of m/z 277; camptothecin at [M + H] + of m/z 371; pseudopelletierine at [M + H] + of m/z 171; Camptothecin at [M + H] + of m/z 371; Pseudopelletierine at [M + H] + of m/z 171; Camptothecin at [M + H] + of m/z 371. b Compounds with pharmacological activity isolated and identified in P. tetrastromatica, C. racemosa and T. ornata fractions
Mentions: Following determination of cytotoxic activity, the pooled fractions P2 of P. tetrastromatica, P1 of C. racemosa and P1 of T. ornata were subjected to LC-MS analysis. Analysis of the mass spectral data for peaks at m/z 371 revealed the presence of camptothecin eluted at 26.5 min. Pseudopelletierine eluted at 12.7 min and lycodine at 20.5 min, showed fragments at m/z 171 and 277, respectively (Fig. 2a). The compounds detected are summarised in Fig. 2b.Fig. 2

Bottom Line: The activity of the cellular antioxidant enzymes, superoxide dismutase, catalase and glutathione reductase, in MCF-7 cells, decreased in a time-dependent manner.LC-MS analysis revealed the presence of bioactive alkaloids such as camptothecin, lycodine and pesudopelletierine.Based on the results obtained, all three seaweeds are rich sources of enzymatic and non-enzymatic antioxidants which could contribute to their reported medicinal benefits.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. gracechia1986@hotmail.com.

ABSTRACT

Background: Three species of seaweeds (Padina tetrastromatica, Caulerpa racemosa and Turbinaria ornata) are widely consumed by Asians as nutraceutical food due to their antioxidant properties. Studies have shown that these seaweeds exhibit bioactivities which include antimicrobial, antiviral, anti-hypertensive and anticoagulant activities. However, investigations into the mechanisms of action pertaining to the cytotoxic activity of the seaweeds are limited. The aim of this study was to determine the antioxidant and cytotoxic activities of whole extracts of P. tetrastromatica, C. racemosa and T. ornata, including the cellular events leading to the apoptotic cell death of the extract treated-MCF-7 cells. Bioassay guided fractionation was carried out and the compounds identified.

Methods: Powdered samples were sequentially extracted for 24 h. Their antioxidant activities were assessed by the DPPH radical, superoxide, nitric oxide and hydroxyl radical scavenging assays. The cytotoxic activity of the extract-treated MCF-7cells was assessed using the MTT assay. The most potent fraction was subjected to bioassay guided fractionation with column chromatography. All the fractions were tested for cytotoxic activity, caspase activity and effect on DNA fragmentation.

Results: All three seaweeds showed potent radical scavenging activities in the various assays. The activity of the cellular antioxidant enzymes, superoxide dismutase, catalase and glutathione reductase, in MCF-7 cells, decreased in a time-dependent manner. The partially purified fractions exhibited higher cytotoxic activity, as assessed by the MTT assay, than the whole extracts in the breast adenocarcinoma cell line, MCF-7. LC-MS analysis revealed the presence of bioactive alkaloids such as camptothecin, lycodine and pesudopelletierine.

Conclusion: Based on the results obtained, all three seaweeds are rich sources of enzymatic and non-enzymatic antioxidants which could contribute to their reported medicinal benefits.

No MeSH data available.


Related in: MedlinePlus