Inositol-phosphodihydroceramides in the periodontal pathogen Tannerella forsythia: Structural analysis and incorporation of exogenous myo-inositol.
Bottom Line: T. forsythia lacks the enzymatic machinery required for myo-inositol synthesis but was found to internalize inositol from the medium for the synthesis of both Tf GL1 and Tf GL2.Their synthesis could be reliant on an external source of myo-inositol.The effects of these unique lipids on the immune system and their role in bacterial virulence could be relevant in the search for new drug targets.
Affiliation: Department of NanoBiotechnology, NanoGlycobiology unit, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Vienna, Austria.Show MeSH
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Mentions: Quantification of free inositol in the medium revealed its presence at an approximate concentration of 400 μM, as estimated by using an external myo-inositol standard. D6-labeled myo-inositol was then added to the medium before inoculation to give a ratio between deuterated and unlabeled inositol of close to 1:1. The ratio at the beginning of the culture and after harvesting the biomass was experimentally determined as 1.05 and 1.03, respectively. Tf GL2 was then purified and subjected to ESI–MS analysis. The ratio between deuterated and unlabeled Tf GL2 was found to be 1.11 (Fig. 8), demonstrating that the isotopic distribution of D6-labeled inositol in Tf GL2 was practically equal to that in the medium. This provides evidence that the incorporated inositol is exclusively derived from the medium.
Affiliation: Department of NanoBiotechnology, NanoGlycobiology unit, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Vienna, Austria.