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Inositol-phosphodihydroceramides in the periodontal pathogen Tannerella forsythia: Structural analysis and incorporation of exogenous myo-inositol.

Megson ZA, Pittenauer E, Duda KA, Engel R, Ortmayr K, Koellensperger G, Mach L, Allmaier G, Holst O, Messner P, Schäffer C - Biochim. Biophys. Acta (2015)

Bottom Line: T. forsythia lacks the enzymatic machinery required for myo-inositol synthesis but was found to internalize inositol from the medium for the synthesis of both Tf GL1 and Tf GL2.Their synthesis could be reliant on an external source of myo-inositol.The effects of these unique lipids on the immune system and their role in bacterial virulence could be relevant in the search for new drug targets.

View Article: PubMed Central - PubMed

Affiliation: Department of NanoBiotechnology, NanoGlycobiology unit, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Vienna, Austria.

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Negative-ion ESI low-energy CID–MS2 of the Tf GL1 [M–H]− precursor ions: m/z 782 (A), m/z 796 (B), m/z 810 (C) and m/z 824 (D).
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Figure 3: Negative-ion ESI low-energy CID–MS2 of the Tf GL1 [M–H]− precursor ions: m/z 782 (A), m/z 796 (B), m/z 810 (C) and m/z 824 (D).

Mentions: CID–MS2 of Tf GL1 revealed major product ions for all four of the [M2–5–H]− precursor ions (Fig. 3, Table 1). The resulting spectrum for each parent ion included peaks corresponding to the loss of inositol ± H2O, [M–H – (162 / 180)]−, loss of an amide-linked 16:0(3-OH) or 17:0(3-OH) FA, [M–H – (254 or 268)]−, and loss of the inositol and partial loss of the latter FA forming a McLafferty ion, [M–H – (212 or 226)]− [30].


Inositol-phosphodihydroceramides in the periodontal pathogen Tannerella forsythia: Structural analysis and incorporation of exogenous myo-inositol.

Megson ZA, Pittenauer E, Duda KA, Engel R, Ortmayr K, Koellensperger G, Mach L, Allmaier G, Holst O, Messner P, Schäffer C - Biochim. Biophys. Acta (2015)

Negative-ion ESI low-energy CID–MS2 of the Tf GL1 [M–H]− precursor ions: m/z 782 (A), m/z 796 (B), m/z 810 (C) and m/z 824 (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4587543&req=5

Figure 3: Negative-ion ESI low-energy CID–MS2 of the Tf GL1 [M–H]− precursor ions: m/z 782 (A), m/z 796 (B), m/z 810 (C) and m/z 824 (D).
Mentions: CID–MS2 of Tf GL1 revealed major product ions for all four of the [M2–5–H]− precursor ions (Fig. 3, Table 1). The resulting spectrum for each parent ion included peaks corresponding to the loss of inositol ± H2O, [M–H – (162 / 180)]−, loss of an amide-linked 16:0(3-OH) or 17:0(3-OH) FA, [M–H – (254 or 268)]−, and loss of the inositol and partial loss of the latter FA forming a McLafferty ion, [M–H – (212 or 226)]− [30].

Bottom Line: T. forsythia lacks the enzymatic machinery required for myo-inositol synthesis but was found to internalize inositol from the medium for the synthesis of both Tf GL1 and Tf GL2.Their synthesis could be reliant on an external source of myo-inositol.The effects of these unique lipids on the immune system and their role in bacterial virulence could be relevant in the search for new drug targets.

View Article: PubMed Central - PubMed

Affiliation: Department of NanoBiotechnology, NanoGlycobiology unit, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Vienna, Austria.

Show MeSH
Related in: MedlinePlus