Inositol-phosphodihydroceramides in the periodontal pathogen Tannerella forsythia: Structural analysis and incorporation of exogenous myo-inositol.
Bottom Line: T. forsythia lacks the enzymatic machinery required for myo-inositol synthesis but was found to internalize inositol from the medium for the synthesis of both Tf GL1 and Tf GL2.Their synthesis could be reliant on an external source of myo-inositol.The effects of these unique lipids on the immune system and their role in bacterial virulence could be relevant in the search for new drug targets.
Affiliation: Department of NanoBiotechnology, NanoGlycobiology unit, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Vienna, Austria.Show MeSH
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Mentions: HPTLC of the Bligh–Dyer extracts from T. forsythia cells followed by a first screening of the primuline-stained bands using MALDI–MS (data not shown) allowed for the identification of the most abundant lipids present (phosphatidylethanolamine, monogalactosyl diacylglycerol, phosphatidic acid and cardiolipin) as well as of the phosphorylated DHC lipids previously reported by Nichols et al. . In addition, we detected two novel DHC lipids which could be resolved with the solvent system A. The less abundant, more polar lipid, designated as Tf GL1, migrated with an Rf value of 0.10 and the second, more abundant lipid, designated here as Tf GL2, migrated slightly in front of Tf GL1 with an Rf value of 0.15 (Fig. 1A).
Affiliation: Department of NanoBiotechnology, NanoGlycobiology unit, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Vienna, Austria.