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Evaluation of an Epitypified Ophiocordyceps formosana (Cordyceps s.l.) for Its Pharmacological Potential.

Wang YW, Hong TW, Tai YL, Wang YJ, Tsai SH, Lien PT, Chou TH, Lai JY, Chu R, Ding ST, Irie K, Li TK, Tzean SS, Shen TL - Evid Based Complement Alternat Med (2015)

Bottom Line: The relative amounts of the known bioactive ingredients that are unique to Cordyceps s.l. species in O. formosana were found to be similar to the amounts in O. sinensis and C. militaris, indicating the potential applicability of O. formosana for pharmacological uses.Additionally, we found that O. formosana exhibited antioxidation activities in vitro and in vivo that were similar to those of O. sinensis and C. militaris.Furthermore, O. formosana also displayed conspicuously effective antitumor activity compared with the tested Cordyceps s.l. species.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei 10617, Taiwan.

ABSTRACT
The substantial merit of Cordyceps s.l. spp. in terms of medicinal benefits is largely appreciated. Nevertheless, only few studies have characterized and examined the clinical complications of the use of health tonics containing these species. Here, we epitypified C. formosana isolates that were collected and characterized as Ophiocordyceps formosana based on morphological characteristics, molecular phylogenetic analyses, and metabolite profiling. Thus, we renamed and transferred C. formosana to the new protologue Ophiocordyceps formosana (Kobayasi & Shimizu) Wang, Tsai, Tzean & Shen comb. nov. Additionally, the pharmacological potential of O. formosana was evaluated based on the hot-water extract from its mycelium. The relative amounts of the known bioactive ingredients that are unique to Cordyceps s.l. species in O. formosana were found to be similar to the amounts in O. sinensis and C. militaris, indicating the potential applicability of O. formosana for pharmacological uses. Additionally, we found that O. formosana exhibited antioxidation activities in vitro and in vivo that were similar to those of O. sinensis and C. militaris. Furthermore, O. formosana also displayed conspicuously effective antitumor activity compared with the tested Cordyceps s.l. species. Intrinsically, O. formosana exhibited less toxicity than the other Cordyceps species. Together, our data suggest that the metabolites of O. formosana may play active roles in complementary medicine.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of Cordyceps s.l. Phylogenetic cladogram of Cordyceps s.l. showing the three newly created families, Clavicipitaceae s.s., Cordycipitaceae, and Ophiocordycipitaceae, as inferred by the maximum likelihood (ML) method and Bayesian inference of the phylogeny using three concatenated genes (RPB1, RPB2, and EF1-α). The bootstrap support values are denoted on the branches in front of the posterior probabilities. The underlined Ophiocordyceps formosana NTU 00035 and MUCHO 815 samples were collected from Taiwan in this study.
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Related In: Results  -  Collection


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fig2: Phylogenetic tree of Cordyceps s.l. Phylogenetic cladogram of Cordyceps s.l. showing the three newly created families, Clavicipitaceae s.s., Cordycipitaceae, and Ophiocordycipitaceae, as inferred by the maximum likelihood (ML) method and Bayesian inference of the phylogeny using three concatenated genes (RPB1, RPB2, and EF1-α). The bootstrap support values are denoted on the branches in front of the posterior probabilities. The underlined Ophiocordyceps formosana NTU 00035 and MUCHO 815 samples were collected from Taiwan in this study.

Mentions: Indeed, the phylogenetic trees based on the maximum likelihood method and Bayesian principles resulted in two identical trees with different support strengths. A maximum likelihood bootstrap tree is shown in Figure 2, and the posterior probability ML bootstrap support values are denoted after the slashes. The topologies of Cordyceps s.l. in the three newly created families, Ophiocordycipitaceae, Cordycipitaceae, and Clavicipitaceae s.s., were largely congruent with the results of Sung et al. [21]. O. formosana resided within the Ophiocordycipitaceae clade with a strong bootstrap support value (BS = 10/PP = 1) rather than in the other allied taxa of Cordycipitaceae and Clavicipitaceae s.s. Phylogenetically, O. formosana obviously exhibited a closer relatedness to O. sinensis than to C. militaris. However, in contrast, C. militaris was placed in the Cordycipitaceae clade, which was distantly related to O. sinensis and O. formosana. Intriguingly, the O. formosana collected in Taiwan from the varied geographic regions in this study were in the clade of O. formosana isolated by another group. Based on the unambiguous morphological traits and molecular phylogenetic study, we recombined the previously erected Cordyceps formosana as Ophiocordyceps formosana, and the epitypic specimen was designated as O. formosana MUCHO 815.


Evaluation of an Epitypified Ophiocordyceps formosana (Cordyceps s.l.) for Its Pharmacological Potential.

Wang YW, Hong TW, Tai YL, Wang YJ, Tsai SH, Lien PT, Chou TH, Lai JY, Chu R, Ding ST, Irie K, Li TK, Tzean SS, Shen TL - Evid Based Complement Alternat Med (2015)

Phylogenetic tree of Cordyceps s.l. Phylogenetic cladogram of Cordyceps s.l. showing the three newly created families, Clavicipitaceae s.s., Cordycipitaceae, and Ophiocordycipitaceae, as inferred by the maximum likelihood (ML) method and Bayesian inference of the phylogeny using three concatenated genes (RPB1, RPB2, and EF1-α). The bootstrap support values are denoted on the branches in front of the posterior probabilities. The underlined Ophiocordyceps formosana NTU 00035 and MUCHO 815 samples were collected from Taiwan in this study.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4587430&req=5

fig2: Phylogenetic tree of Cordyceps s.l. Phylogenetic cladogram of Cordyceps s.l. showing the three newly created families, Clavicipitaceae s.s., Cordycipitaceae, and Ophiocordycipitaceae, as inferred by the maximum likelihood (ML) method and Bayesian inference of the phylogeny using three concatenated genes (RPB1, RPB2, and EF1-α). The bootstrap support values are denoted on the branches in front of the posterior probabilities. The underlined Ophiocordyceps formosana NTU 00035 and MUCHO 815 samples were collected from Taiwan in this study.
Mentions: Indeed, the phylogenetic trees based on the maximum likelihood method and Bayesian principles resulted in two identical trees with different support strengths. A maximum likelihood bootstrap tree is shown in Figure 2, and the posterior probability ML bootstrap support values are denoted after the slashes. The topologies of Cordyceps s.l. in the three newly created families, Ophiocordycipitaceae, Cordycipitaceae, and Clavicipitaceae s.s., were largely congruent with the results of Sung et al. [21]. O. formosana resided within the Ophiocordycipitaceae clade with a strong bootstrap support value (BS = 10/PP = 1) rather than in the other allied taxa of Cordycipitaceae and Clavicipitaceae s.s. Phylogenetically, O. formosana obviously exhibited a closer relatedness to O. sinensis than to C. militaris. However, in contrast, C. militaris was placed in the Cordycipitaceae clade, which was distantly related to O. sinensis and O. formosana. Intriguingly, the O. formosana collected in Taiwan from the varied geographic regions in this study were in the clade of O. formosana isolated by another group. Based on the unambiguous morphological traits and molecular phylogenetic study, we recombined the previously erected Cordyceps formosana as Ophiocordyceps formosana, and the epitypic specimen was designated as O. formosana MUCHO 815.

Bottom Line: The relative amounts of the known bioactive ingredients that are unique to Cordyceps s.l. species in O. formosana were found to be similar to the amounts in O. sinensis and C. militaris, indicating the potential applicability of O. formosana for pharmacological uses.Additionally, we found that O. formosana exhibited antioxidation activities in vitro and in vivo that were similar to those of O. sinensis and C. militaris.Furthermore, O. formosana also displayed conspicuously effective antitumor activity compared with the tested Cordyceps s.l. species.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei 10617, Taiwan.

ABSTRACT
The substantial merit of Cordyceps s.l. spp. in terms of medicinal benefits is largely appreciated. Nevertheless, only few studies have characterized and examined the clinical complications of the use of health tonics containing these species. Here, we epitypified C. formosana isolates that were collected and characterized as Ophiocordyceps formosana based on morphological characteristics, molecular phylogenetic analyses, and metabolite profiling. Thus, we renamed and transferred C. formosana to the new protologue Ophiocordyceps formosana (Kobayasi & Shimizu) Wang, Tsai, Tzean & Shen comb. nov. Additionally, the pharmacological potential of O. formosana was evaluated based on the hot-water extract from its mycelium. The relative amounts of the known bioactive ingredients that are unique to Cordyceps s.l. species in O. formosana were found to be similar to the amounts in O. sinensis and C. militaris, indicating the potential applicability of O. formosana for pharmacological uses. Additionally, we found that O. formosana exhibited antioxidation activities in vitro and in vivo that were similar to those of O. sinensis and C. militaris. Furthermore, O. formosana also displayed conspicuously effective antitumor activity compared with the tested Cordyceps s.l. species. Intrinsically, O. formosana exhibited less toxicity than the other Cordyceps species. Together, our data suggest that the metabolites of O. formosana may play active roles in complementary medicine.

No MeSH data available.


Related in: MedlinePlus