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Glycomic Analysis of Life Stages of the Human Parasite Schistosoma mansoni Reveals Developmental Expression Profiles of Functional and Antigenic Glycan Motifs.

Smit CH, van Diepen A, Nguyen DL, Wuhrer M, Hoffmann KF, Deelder AM, Hokke CH - Mol. Cell Proteomics (2015)

Bottom Line: O-glycans in the mature eggs were also diverse and contained LeX- and multifucosylated LDN, but none of these were associated with miracidia in which we detected only the Galβ1-3(Galβ1-6)GalNAc core glycan.Lipid glycans with multifucosylated GlcNAc repeats were present throughout egg development, but with the longer highly fucosylated stretches enriched in mature eggs and miracidia.This global analysis of the developing schistosome's glycome provides new insights into how stage-specifically expressed glycans may contribute to different aspects of schistosome-host interactions.

View Article: PubMed Central - PubMed

Affiliation: From the ‡Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands;

No MeSH data available.


Related in: MedlinePlus

MALDI-TOF-MS of the permethylated O-glycans obtained by reductive β-elimination from S. mansoni cercariae, A, immature eggs, B, mature eggs, C, and miracidia, D. Glycans were analyzed as sodium-adducts in positive-ion reflectron mode. All signals are labeled with monoisotopic masses and structures based on the results of MALDI-TOF MS/MS fragmentations (masses indicated in black) supplemented with literature data (masses indicated in red). Red triangle, fucose; yellow circle, galactose; blue square, N-acetylglucosamine; yellow square, N-acetylgalactosamine. Signals corresponding to a hexose oligomer of unknown origin are marked with *. Nonglycan signals are marked with #.
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Figure 4: MALDI-TOF-MS of the permethylated O-glycans obtained by reductive β-elimination from S. mansoni cercariae, A, immature eggs, B, mature eggs, C, and miracidia, D. Glycans were analyzed as sodium-adducts in positive-ion reflectron mode. All signals are labeled with monoisotopic masses and structures based on the results of MALDI-TOF MS/MS fragmentations (masses indicated in black) supplemented with literature data (masses indicated in red). Red triangle, fucose; yellow circle, galactose; blue square, N-acetylglucosamine; yellow square, N-acetylgalactosamine. Signals corresponding to a hexose oligomer of unknown origin are marked with *. Nonglycan signals are marked with #.

Mentions: O-glycans were obtained by reductive β-elimination of protein extracts from various schistosome life stages and permethylated prior to MS analysis. Glycan structures were assigned on the basis of compositions and MALDI-TOF-MS/MS fragmentations, taking into account previously published data when available. O-glycan spectra of the life stages studied are shown in Fig. 4A–4D and supplemental Fig. S2A–S2H. Examples of structural characterizations by MALDI-TOF-MS/MS are shown in supplemental Fig. S3.


Glycomic Analysis of Life Stages of the Human Parasite Schistosoma mansoni Reveals Developmental Expression Profiles of Functional and Antigenic Glycan Motifs.

Smit CH, van Diepen A, Nguyen DL, Wuhrer M, Hoffmann KF, Deelder AM, Hokke CH - Mol. Cell Proteomics (2015)

MALDI-TOF-MS of the permethylated O-glycans obtained by reductive β-elimination from S. mansoni cercariae, A, immature eggs, B, mature eggs, C, and miracidia, D. Glycans were analyzed as sodium-adducts in positive-ion reflectron mode. All signals are labeled with monoisotopic masses and structures based on the results of MALDI-TOF MS/MS fragmentations (masses indicated in black) supplemented with literature data (masses indicated in red). Red triangle, fucose; yellow circle, galactose; blue square, N-acetylglucosamine; yellow square, N-acetylgalactosamine. Signals corresponding to a hexose oligomer of unknown origin are marked with *. Nonglycan signals are marked with #.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4587318&req=5

Figure 4: MALDI-TOF-MS of the permethylated O-glycans obtained by reductive β-elimination from S. mansoni cercariae, A, immature eggs, B, mature eggs, C, and miracidia, D. Glycans were analyzed as sodium-adducts in positive-ion reflectron mode. All signals are labeled with monoisotopic masses and structures based on the results of MALDI-TOF MS/MS fragmentations (masses indicated in black) supplemented with literature data (masses indicated in red). Red triangle, fucose; yellow circle, galactose; blue square, N-acetylglucosamine; yellow square, N-acetylgalactosamine. Signals corresponding to a hexose oligomer of unknown origin are marked with *. Nonglycan signals are marked with #.
Mentions: O-glycans were obtained by reductive β-elimination of protein extracts from various schistosome life stages and permethylated prior to MS analysis. Glycan structures were assigned on the basis of compositions and MALDI-TOF-MS/MS fragmentations, taking into account previously published data when available. O-glycan spectra of the life stages studied are shown in Fig. 4A–4D and supplemental Fig. S2A–S2H. Examples of structural characterizations by MALDI-TOF-MS/MS are shown in supplemental Fig. S3.

Bottom Line: O-glycans in the mature eggs were also diverse and contained LeX- and multifucosylated LDN, but none of these were associated with miracidia in which we detected only the Galβ1-3(Galβ1-6)GalNAc core glycan.Lipid glycans with multifucosylated GlcNAc repeats were present throughout egg development, but with the longer highly fucosylated stretches enriched in mature eggs and miracidia.This global analysis of the developing schistosome's glycome provides new insights into how stage-specifically expressed glycans may contribute to different aspects of schistosome-host interactions.

View Article: PubMed Central - PubMed

Affiliation: From the ‡Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands;

No MeSH data available.


Related in: MedlinePlus