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Assessment of phytochemicals and antioxidant activities of raw and germinating Ceiba pentandra (kapok) seeds.

Ravi Kiran C, Rao DB, Sirisha N, Rao TR - J Biomed Res (2013)

Bottom Line: The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination.Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds.The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Science and Technology, Andhra University , Visakhapatnam-530003 , India.

ABSTRACT
To determine the significance of germination on phytochemical constituents and non-enzymatic antioxidant activities of Ceiba pentandra seed extracts. Phytochemicals and antioxidant activities of raw and germinating seeds of Ceiba pentandra were estimated by different methods. The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination. Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds. This may be due to various reactive oxygen species (ROS) that were generated as by-products of metabolism during germination. This group of ROS included superoxide radicals (O2), hydrogen peroxide radicals (H2O2) and hydroxyl radicals (OH). The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes. Therefore, it was hypothesized that this could be related to the increase of antioxidant activity in large unilamellar vesicles observed in germinated seeds. The implication of this study is that the Ceiba pentandra seeds as natural antioxidant agents and put forward the possibility of employing for therapeutic potential.

No MeSH data available.


Related in: MedlinePlus

DPPH activity (A), FRAP ability (B), reducing activity (C) and hydroxy radical scavenging activity of (D) Ceiba pentandra seed extracts.
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f02: DPPH activity (A), FRAP ability (B), reducing activity (C) and hydroxy radical scavenging activity of (D) Ceiba pentandra seed extracts.

Mentions: The germination time modified the antioxidant capacity, and all activities of the germinated Ceiba pentandra were increased in a dose dependent manner ranging from 25 to 100 mg/mL. All the outcomes of scrutiny mentioned were at 100 mg/mL, the products for DPPH radical scavenging activity of raw and germinating seeds were 43.5% and 51.3% of inhibition per 50 µL of extract as compared to 45% and 76% of inhibition per 1 mg/mL of Rutin and BHT as positive controls, with t value of 13.440 and significant P value of 0.005 (Fig. 2A).


Assessment of phytochemicals and antioxidant activities of raw and germinating Ceiba pentandra (kapok) seeds.

Ravi Kiran C, Rao DB, Sirisha N, Rao TR - J Biomed Res (2013)

DPPH activity (A), FRAP ability (B), reducing activity (C) and hydroxy radical scavenging activity of (D) Ceiba pentandra seed extracts.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4585437&req=5

f02: DPPH activity (A), FRAP ability (B), reducing activity (C) and hydroxy radical scavenging activity of (D) Ceiba pentandra seed extracts.
Mentions: The germination time modified the antioxidant capacity, and all activities of the germinated Ceiba pentandra were increased in a dose dependent manner ranging from 25 to 100 mg/mL. All the outcomes of scrutiny mentioned were at 100 mg/mL, the products for DPPH radical scavenging activity of raw and germinating seeds were 43.5% and 51.3% of inhibition per 50 µL of extract as compared to 45% and 76% of inhibition per 1 mg/mL of Rutin and BHT as positive controls, with t value of 13.440 and significant P value of 0.005 (Fig. 2A).

Bottom Line: The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination.Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds.The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Science and Technology, Andhra University , Visakhapatnam-530003 , India.

ABSTRACT
To determine the significance of germination on phytochemical constituents and non-enzymatic antioxidant activities of Ceiba pentandra seed extracts. Phytochemicals and antioxidant activities of raw and germinating seeds of Ceiba pentandra were estimated by different methods. The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination. Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds. This may be due to various reactive oxygen species (ROS) that were generated as by-products of metabolism during germination. This group of ROS included superoxide radicals (O2), hydrogen peroxide radicals (H2O2) and hydroxyl radicals (OH). The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes. Therefore, it was hypothesized that this could be related to the increase of antioxidant activity in large unilamellar vesicles observed in germinated seeds. The implication of this study is that the Ceiba pentandra seeds as natural antioxidant agents and put forward the possibility of employing for therapeutic potential.

No MeSH data available.


Related in: MedlinePlus