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Assessment of phytochemicals and antioxidant activities of raw and germinating Ceiba pentandra (kapok) seeds.

Ravi Kiran C, Rao DB, Sirisha N, Rao TR - J Biomed Res (2013)

Bottom Line: The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination.Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds.The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Science and Technology, Andhra University , Visakhapatnam-530003 , India.

ABSTRACT
To determine the significance of germination on phytochemical constituents and non-enzymatic antioxidant activities of Ceiba pentandra seed extracts. Phytochemicals and antioxidant activities of raw and germinating seeds of Ceiba pentandra were estimated by different methods. The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination. Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds. This may be due to various reactive oxygen species (ROS) that were generated as by-products of metabolism during germination. This group of ROS included superoxide radicals (O2), hydrogen peroxide radicals (H2O2) and hydroxyl radicals (OH). The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes. Therefore, it was hypothesized that this could be related to the increase of antioxidant activity in large unilamellar vesicles observed in germinated seeds. The implication of this study is that the Ceiba pentandra seeds as natural antioxidant agents and put forward the possibility of employing for therapeutic potential.

No MeSH data available.


Related in: MedlinePlus

Phenolic (A), flavonoid (B), alkaloid (C) and tannin (D) content of Ceiba pentandra seed extracts.
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f01: Phenolic (A), flavonoid (B), alkaloid (C) and tannin (D) content of Ceiba pentandra seed extracts.

Mentions: The primary objective of germination was to promote the development of hydrolytic enzymes that were inactive in raw seeds. Even though the success rate of germination was low in our study, the germination percentage of Ceiba pentandra seeds increased with the increase in germination time[17]. The tested levels of phytochemical constituents were influenced by germination and amplified in the increasing concentration from 25 mg/mL to 100 mg/mL except two constituents, including alkaloids and tannins, which were decreased significantly during germination. All the results of analysis mentioned below were at 100 mg/mL. The total phenolic content of germinating seeds was 1,303 µg gallic acid equivalents/g of extract, whereas for raw seeds it was 802.7 µg gallic acid equivalents/g of extract, with t value of 867.757, along with significant P value of 0.000 (2-tailed). The findings are shown in Fig. 1A.


Assessment of phytochemicals and antioxidant activities of raw and germinating Ceiba pentandra (kapok) seeds.

Ravi Kiran C, Rao DB, Sirisha N, Rao TR - J Biomed Res (2013)

Phenolic (A), flavonoid (B), alkaloid (C) and tannin (D) content of Ceiba pentandra seed extracts.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4585437&req=5

f01: Phenolic (A), flavonoid (B), alkaloid (C) and tannin (D) content of Ceiba pentandra seed extracts.
Mentions: The primary objective of germination was to promote the development of hydrolytic enzymes that were inactive in raw seeds. Even though the success rate of germination was low in our study, the germination percentage of Ceiba pentandra seeds increased with the increase in germination time[17]. The tested levels of phytochemical constituents were influenced by germination and amplified in the increasing concentration from 25 mg/mL to 100 mg/mL except two constituents, including alkaloids and tannins, which were decreased significantly during germination. All the results of analysis mentioned below were at 100 mg/mL. The total phenolic content of germinating seeds was 1,303 µg gallic acid equivalents/g of extract, whereas for raw seeds it was 802.7 µg gallic acid equivalents/g of extract, with t value of 867.757, along with significant P value of 0.000 (2-tailed). The findings are shown in Fig. 1A.

Bottom Line: The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination.Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds.The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Science and Technology, Andhra University , Visakhapatnam-530003 , India.

ABSTRACT
To determine the significance of germination on phytochemical constituents and non-enzymatic antioxidant activities of Ceiba pentandra seed extracts. Phytochemicals and antioxidant activities of raw and germinating seeds of Ceiba pentandra were estimated by different methods. The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination. Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds. This may be due to various reactive oxygen species (ROS) that were generated as by-products of metabolism during germination. This group of ROS included superoxide radicals (O2), hydrogen peroxide radicals (H2O2) and hydroxyl radicals (OH). The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes. Therefore, it was hypothesized that this could be related to the increase of antioxidant activity in large unilamellar vesicles observed in germinated seeds. The implication of this study is that the Ceiba pentandra seeds as natural antioxidant agents and put forward the possibility of employing for therapeutic potential.

No MeSH data available.


Related in: MedlinePlus