Limits...
Diacerein protects against iodoacetate-induced osteoarthritis in the femorotibial joints of rats.

Jain A, Singh R, Singh S, Singh S - J Biomed Res (2015)

Bottom Line: Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect.Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection).Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Indian Institute of Technology ; National Institute of Pharmaceutical Education & Research , Raebareli-229 010, U.P. India.

ABSTRACT
The present study was undertaken to investigate the effect of diacerein on the histopathology of articular cartilage and subchondral bone of the femorotibial joint in rats. Osteoarthritis was induced in rats after single intra-articular injection of sodium iodoacetate. Rats were sacrificed 1, 2, 4, and 8 weeks post intra-articular injection to evaluate the progression of histopathogenesis of osteoarthritis. Diacerein was orally administered (15 mg/kg) once daily post 1 and 2 weeks of iodoacetate injection in two groups, respectively, for up to 12 weeks. Articular cartilage and subchondral bone of the rats of both groups were examined after 8 and 12 weeks, respectively. Quantitative histological analyses were performed by scoring these sections as per the OARSI system. Chondroitin sulfate was also estimated in articular cartilage by decrease in absorbance of methylene blue on complexation with chondroitin sulfate using a spectrophotometer. Intra-articular injection of iodoacetate induced loss of articular cartilage with progressive subchondral bone sclerosis and degeneration. Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect. Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection). Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect. In conclusion, diacerein exhibits chondroprotective effect in rats with late onset of action.

No MeSH data available.


Related in: MedlinePlus

Concentrations of CS in the articular cartilage of the treatment groups.All values are reported as Mean±SD (n = 4; ANOVA), *P≤0.01 compared to Group 2 (arthritic control; post 7th day IA injection); †P≤0.05 compared to Group 3 (arthritic control; post 14th day IA injection); **P≤0.001 compared to all other group except Group 7.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4585436&req=5

f05: Concentrations of CS in the articular cartilage of the treatment groups.All values are reported as Mean±SD (n = 4; ANOVA), *P≤0.01 compared to Group 2 (arthritic control; post 7th day IA injection); †P≤0.05 compared to Group 3 (arthritic control; post 14th day IA injection); **P≤0.001 compared to all other group except Group 7.

Mentions: OA induction caused significant reduction in chondroitin sulfate concentration in a time dependent manner. Chondroitin sulfate concentration in the articular cartilages of diacerein treated rats was found to be higher, compared to the control rats (i. Further, chondroitin sulfate concentration was even higher after treatment for 12 weeks, compared to 8 weeks (Fig. 5).


Diacerein protects against iodoacetate-induced osteoarthritis in the femorotibial joints of rats.

Jain A, Singh R, Singh S, Singh S - J Biomed Res (2015)

Concentrations of CS in the articular cartilage of the treatment groups.All values are reported as Mean±SD (n = 4; ANOVA), *P≤0.01 compared to Group 2 (arthritic control; post 7th day IA injection); †P≤0.05 compared to Group 3 (arthritic control; post 14th day IA injection); **P≤0.001 compared to all other group except Group 7.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4585436&req=5

f05: Concentrations of CS in the articular cartilage of the treatment groups.All values are reported as Mean±SD (n = 4; ANOVA), *P≤0.01 compared to Group 2 (arthritic control; post 7th day IA injection); †P≤0.05 compared to Group 3 (arthritic control; post 14th day IA injection); **P≤0.001 compared to all other group except Group 7.
Mentions: OA induction caused significant reduction in chondroitin sulfate concentration in a time dependent manner. Chondroitin sulfate concentration in the articular cartilages of diacerein treated rats was found to be higher, compared to the control rats (i. Further, chondroitin sulfate concentration was even higher after treatment for 12 weeks, compared to 8 weeks (Fig. 5).

Bottom Line: Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect.Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection).Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Indian Institute of Technology ; National Institute of Pharmaceutical Education & Research , Raebareli-229 010, U.P. India.

ABSTRACT
The present study was undertaken to investigate the effect of diacerein on the histopathology of articular cartilage and subchondral bone of the femorotibial joint in rats. Osteoarthritis was induced in rats after single intra-articular injection of sodium iodoacetate. Rats were sacrificed 1, 2, 4, and 8 weeks post intra-articular injection to evaluate the progression of histopathogenesis of osteoarthritis. Diacerein was orally administered (15 mg/kg) once daily post 1 and 2 weeks of iodoacetate injection in two groups, respectively, for up to 12 weeks. Articular cartilage and subchondral bone of the rats of both groups were examined after 8 and 12 weeks, respectively. Quantitative histological analyses were performed by scoring these sections as per the OARSI system. Chondroitin sulfate was also estimated in articular cartilage by decrease in absorbance of methylene blue on complexation with chondroitin sulfate using a spectrophotometer. Intra-articular injection of iodoacetate induced loss of articular cartilage with progressive subchondral bone sclerosis and degeneration. Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect. Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection). Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect. In conclusion, diacerein exhibits chondroprotective effect in rats with late onset of action.

No MeSH data available.


Related in: MedlinePlus