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Diacerein protects against iodoacetate-induced osteoarthritis in the femorotibial joints of rats.

Jain A, Singh R, Singh S, Singh S - J Biomed Res (2015)

Bottom Line: Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect.Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection).Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Indian Institute of Technology ; National Institute of Pharmaceutical Education & Research , Raebareli-229 010, U.P. India.

ABSTRACT
The present study was undertaken to investigate the effect of diacerein on the histopathology of articular cartilage and subchondral bone of the femorotibial joint in rats. Osteoarthritis was induced in rats after single intra-articular injection of sodium iodoacetate. Rats were sacrificed 1, 2, 4, and 8 weeks post intra-articular injection to evaluate the progression of histopathogenesis of osteoarthritis. Diacerein was orally administered (15 mg/kg) once daily post 1 and 2 weeks of iodoacetate injection in two groups, respectively, for up to 12 weeks. Articular cartilage and subchondral bone of the rats of both groups were examined after 8 and 12 weeks, respectively. Quantitative histological analyses were performed by scoring these sections as per the OARSI system. Chondroitin sulfate was also estimated in articular cartilage by decrease in absorbance of methylene blue on complexation with chondroitin sulfate using a spectrophotometer. Intra-articular injection of iodoacetate induced loss of articular cartilage with progressive subchondral bone sclerosis and degeneration. Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect. Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection). Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect. In conclusion, diacerein exhibits chondroprotective effect in rats with late onset of action.

No MeSH data available.


Related in: MedlinePlus

Effect of treatment of diacerein after post 1 week IA injection.A: Femorotibial joint 1 week post IA injection showing mild to moderate OA changes. Note the degenerating chondrocyte (Arrow) and loss of the cellular matrix; (Grade 2.5, Stage 2). B: Eight weeks after DC treatment which begun 1 week post IA injection. Regeneration of the cartilage is evident by the cell nest (arrow) within the articular cartilage. Cortical bone formation with microvasculature evident (by bold Arrow head); (Grade 2, Stage 2). C: Twelve weeks after DC treatment which begun 1 week post IA injection. Cell cartilage is well-organized; (Grade 1.5, Stage 2) H & E. x400.
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f03: Effect of treatment of diacerein after post 1 week IA injection.A: Femorotibial joint 1 week post IA injection showing mild to moderate OA changes. Note the degenerating chondrocyte (Arrow) and loss of the cellular matrix; (Grade 2.5, Stage 2). B: Eight weeks after DC treatment which begun 1 week post IA injection. Regeneration of the cartilage is evident by the cell nest (arrow) within the articular cartilage. Cortical bone formation with microvasculature evident (by bold Arrow head); (Grade 2, Stage 2). C: Twelve weeks after DC treatment which begun 1 week post IA injection. Cell cartilage is well-organized; (Grade 1.5, Stage 2) H & E. x400.

Mentions: Diacerein treated rats, when compared with the control rats, had the same degree of OA at 1 and 2 weeks post injection of iodoacetate (Fig. 3 and 4). The femoro-tibial joints exhibited early to moderate levels of OA 1 week post iodoacetate injection (Fig. 3A). Healing was evident by 8 weeks post diacerein treatment and the degree of OA changes regressed at 12 weeks (Fig. 3B and C). Degenerating chondrocytes were not seen in diacereinrats. Similarly, the femorotibial joint, 2 weeks post iodoacetate injection, showed moderate-to-advanced OA and was compared with diacerein treated rats (Fig. 4A). Regeneration of cartilage, as evident by new chondrocyte cell nests and other structure modifications, was evident after diacerein treatment (Fig. 4B, C and D). The degree of regeneration was better at 12 weeks than at 8 weeks. The chondrocyte regeneration was at faster rate than the degeneration, evidenced by pyknotic chondrocytes seen at both 8 and 12 weeks. The chondrocytes exhibited regeneration. Finally, the score values for quantitative histological analyses are summarized in Table 1. Scores for each section were calculated on the basis of individual grading values and staging of respective sections.


Diacerein protects against iodoacetate-induced osteoarthritis in the femorotibial joints of rats.

Jain A, Singh R, Singh S, Singh S - J Biomed Res (2015)

Effect of treatment of diacerein after post 1 week IA injection.A: Femorotibial joint 1 week post IA injection showing mild to moderate OA changes. Note the degenerating chondrocyte (Arrow) and loss of the cellular matrix; (Grade 2.5, Stage 2). B: Eight weeks after DC treatment which begun 1 week post IA injection. Regeneration of the cartilage is evident by the cell nest (arrow) within the articular cartilage. Cortical bone formation with microvasculature evident (by bold Arrow head); (Grade 2, Stage 2). C: Twelve weeks after DC treatment which begun 1 week post IA injection. Cell cartilage is well-organized; (Grade 1.5, Stage 2) H & E. x400.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4585436&req=5

f03: Effect of treatment of diacerein after post 1 week IA injection.A: Femorotibial joint 1 week post IA injection showing mild to moderate OA changes. Note the degenerating chondrocyte (Arrow) and loss of the cellular matrix; (Grade 2.5, Stage 2). B: Eight weeks after DC treatment which begun 1 week post IA injection. Regeneration of the cartilage is evident by the cell nest (arrow) within the articular cartilage. Cortical bone formation with microvasculature evident (by bold Arrow head); (Grade 2, Stage 2). C: Twelve weeks after DC treatment which begun 1 week post IA injection. Cell cartilage is well-organized; (Grade 1.5, Stage 2) H & E. x400.
Mentions: Diacerein treated rats, when compared with the control rats, had the same degree of OA at 1 and 2 weeks post injection of iodoacetate (Fig. 3 and 4). The femoro-tibial joints exhibited early to moderate levels of OA 1 week post iodoacetate injection (Fig. 3A). Healing was evident by 8 weeks post diacerein treatment and the degree of OA changes regressed at 12 weeks (Fig. 3B and C). Degenerating chondrocytes were not seen in diacereinrats. Similarly, the femorotibial joint, 2 weeks post iodoacetate injection, showed moderate-to-advanced OA and was compared with diacerein treated rats (Fig. 4A). Regeneration of cartilage, as evident by new chondrocyte cell nests and other structure modifications, was evident after diacerein treatment (Fig. 4B, C and D). The degree of regeneration was better at 12 weeks than at 8 weeks. The chondrocyte regeneration was at faster rate than the degeneration, evidenced by pyknotic chondrocytes seen at both 8 and 12 weeks. The chondrocytes exhibited regeneration. Finally, the score values for quantitative histological analyses are summarized in Table 1. Scores for each section were calculated on the basis of individual grading values and staging of respective sections.

Bottom Line: Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect.Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection).Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Indian Institute of Technology ; National Institute of Pharmaceutical Education & Research , Raebareli-229 010, U.P. India.

ABSTRACT
The present study was undertaken to investigate the effect of diacerein on the histopathology of articular cartilage and subchondral bone of the femorotibial joint in rats. Osteoarthritis was induced in rats after single intra-articular injection of sodium iodoacetate. Rats were sacrificed 1, 2, 4, and 8 weeks post intra-articular injection to evaluate the progression of histopathogenesis of osteoarthritis. Diacerein was orally administered (15 mg/kg) once daily post 1 and 2 weeks of iodoacetate injection in two groups, respectively, for up to 12 weeks. Articular cartilage and subchondral bone of the rats of both groups were examined after 8 and 12 weeks, respectively. Quantitative histological analyses were performed by scoring these sections as per the OARSI system. Chondroitin sulfate was also estimated in articular cartilage by decrease in absorbance of methylene blue on complexation with chondroitin sulfate using a spectrophotometer. Intra-articular injection of iodoacetate induced loss of articular cartilage with progressive subchondral bone sclerosis and degeneration. Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect. Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection). Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect. In conclusion, diacerein exhibits chondroprotective effect in rats with late onset of action.

No MeSH data available.


Related in: MedlinePlus