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Diacerein protects against iodoacetate-induced osteoarthritis in the femorotibial joints of rats.

Jain A, Singh R, Singh S, Singh S - J Biomed Res (2015)

Bottom Line: Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect.Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection).Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Indian Institute of Technology ; National Institute of Pharmaceutical Education & Research , Raebareli-229 010, U.P. India.

ABSTRACT
The present study was undertaken to investigate the effect of diacerein on the histopathology of articular cartilage and subchondral bone of the femorotibial joint in rats. Osteoarthritis was induced in rats after single intra-articular injection of sodium iodoacetate. Rats were sacrificed 1, 2, 4, and 8 weeks post intra-articular injection to evaluate the progression of histopathogenesis of osteoarthritis. Diacerein was orally administered (15 mg/kg) once daily post 1 and 2 weeks of iodoacetate injection in two groups, respectively, for up to 12 weeks. Articular cartilage and subchondral bone of the rats of both groups were examined after 8 and 12 weeks, respectively. Quantitative histological analyses were performed by scoring these sections as per the OARSI system. Chondroitin sulfate was also estimated in articular cartilage by decrease in absorbance of methylene blue on complexation with chondroitin sulfate using a spectrophotometer. Intra-articular injection of iodoacetate induced loss of articular cartilage with progressive subchondral bone sclerosis and degeneration. Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect. Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection). Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect. In conclusion, diacerein exhibits chondroprotective effect in rats with late onset of action.

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Comparison of normal femorotibial joint with osteoarthritic joint.A: Photomicrograph of a normal (treated with saline as vehicle) Charles Foster rat tibiofemoral joint. The articular surface is smooth. The femoral condyles are separated by meniscus from the tibial plateau. The normal structure of cartilage is organized in three well-ordered zones: the superficial, the mid zone and the deep zone (S, M, D). The subchondral (SC) bone, articular bone plate (AP) and the trabecular bone (TB) are clearly observed. The trabecular space (TS) is filled with hemopoitic tissue. (Grade 0, Stage 0). H & E A1 x20 (panoramic view), A2 x100, A3 x400. B: Photomicrograph of a treated Charles Foster rat femorotibial joint 1 week post IA injection. The articular space between the two ends has widened. The femoral condyles articular surface shows irregularity (arrow). The tibial plateau shows thinning (arrow head) as well as an irregular hyaline cartilage surface (arrow). The subchondral space show diminished hemopoietic tissue with bony spicule loss; (Grade 2-2.5, Stage 2). H&E x20 (panoramic view).
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f01: Comparison of normal femorotibial joint with osteoarthritic joint.A: Photomicrograph of a normal (treated with saline as vehicle) Charles Foster rat tibiofemoral joint. The articular surface is smooth. The femoral condyles are separated by meniscus from the tibial plateau. The normal structure of cartilage is organized in three well-ordered zones: the superficial, the mid zone and the deep zone (S, M, D). The subchondral (SC) bone, articular bone plate (AP) and the trabecular bone (TB) are clearly observed. The trabecular space (TS) is filled with hemopoitic tissue. (Grade 0, Stage 0). H & E A1 x20 (panoramic view), A2 x100, A3 x400. B: Photomicrograph of a treated Charles Foster rat femorotibial joint 1 week post IA injection. The articular space between the two ends has widened. The femoral condyles articular surface shows irregularity (arrow). The tibial plateau shows thinning (arrow head) as well as an irregular hyaline cartilage surface (arrow). The subchondral space show diminished hemopoietic tissue with bony spicule loss; (Grade 2-2.5, Stage 2). H&E x20 (panoramic view).

Mentions: Normal femorotibial joints were compared with the osteoarthritic joints (Fig. 1). Microphotographs of the control femorotibial joints showed normal articular cartilage (Fig. 1A). The articular surface was smooth and without any fissures or debris attached to it. The femoral condyles were separated by meniscus from the tibial plateau. One week post iodoacetate injection, thinning, irregularity, and loss of surface hyaline cartilage of the joints were observed (Fig. 1B). Widening of the intraarticular space was a common finding in the entire joint injected with iodoacetate. Diminished hemopoietic tissue was found in the trabecular space in the subchondral region; the tidemark representing the boundary between calcified and non-calcified region was also lost. Loss of bony spicules and sclerosis of the subcortical bone was also exhibited.


Diacerein protects against iodoacetate-induced osteoarthritis in the femorotibial joints of rats.

Jain A, Singh R, Singh S, Singh S - J Biomed Res (2015)

Comparison of normal femorotibial joint with osteoarthritic joint.A: Photomicrograph of a normal (treated with saline as vehicle) Charles Foster rat tibiofemoral joint. The articular surface is smooth. The femoral condyles are separated by meniscus from the tibial plateau. The normal structure of cartilage is organized in three well-ordered zones: the superficial, the mid zone and the deep zone (S, M, D). The subchondral (SC) bone, articular bone plate (AP) and the trabecular bone (TB) are clearly observed. The trabecular space (TS) is filled with hemopoitic tissue. (Grade 0, Stage 0). H & E A1 x20 (panoramic view), A2 x100, A3 x400. B: Photomicrograph of a treated Charles Foster rat femorotibial joint 1 week post IA injection. The articular space between the two ends has widened. The femoral condyles articular surface shows irregularity (arrow). The tibial plateau shows thinning (arrow head) as well as an irregular hyaline cartilage surface (arrow). The subchondral space show diminished hemopoietic tissue with bony spicule loss; (Grade 2-2.5, Stage 2). H&E x20 (panoramic view).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4585436&req=5

f01: Comparison of normal femorotibial joint with osteoarthritic joint.A: Photomicrograph of a normal (treated with saline as vehicle) Charles Foster rat tibiofemoral joint. The articular surface is smooth. The femoral condyles are separated by meniscus from the tibial plateau. The normal structure of cartilage is organized in three well-ordered zones: the superficial, the mid zone and the deep zone (S, M, D). The subchondral (SC) bone, articular bone plate (AP) and the trabecular bone (TB) are clearly observed. The trabecular space (TS) is filled with hemopoitic tissue. (Grade 0, Stage 0). H & E A1 x20 (panoramic view), A2 x100, A3 x400. B: Photomicrograph of a treated Charles Foster rat femorotibial joint 1 week post IA injection. The articular space between the two ends has widened. The femoral condyles articular surface shows irregularity (arrow). The tibial plateau shows thinning (arrow head) as well as an irregular hyaline cartilage surface (arrow). The subchondral space show diminished hemopoietic tissue with bony spicule loss; (Grade 2-2.5, Stage 2). H&E x20 (panoramic view).
Mentions: Normal femorotibial joints were compared with the osteoarthritic joints (Fig. 1). Microphotographs of the control femorotibial joints showed normal articular cartilage (Fig. 1A). The articular surface was smooth and without any fissures or debris attached to it. The femoral condyles were separated by meniscus from the tibial plateau. One week post iodoacetate injection, thinning, irregularity, and loss of surface hyaline cartilage of the joints were observed (Fig. 1B). Widening of the intraarticular space was a common finding in the entire joint injected with iodoacetate. Diminished hemopoietic tissue was found in the trabecular space in the subchondral region; the tidemark representing the boundary between calcified and non-calcified region was also lost. Loss of bony spicules and sclerosis of the subcortical bone was also exhibited.

Bottom Line: Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect.Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection).Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Indian Institute of Technology ; National Institute of Pharmaceutical Education & Research , Raebareli-229 010, U.P. India.

ABSTRACT
The present study was undertaken to investigate the effect of diacerein on the histopathology of articular cartilage and subchondral bone of the femorotibial joint in rats. Osteoarthritis was induced in rats after single intra-articular injection of sodium iodoacetate. Rats were sacrificed 1, 2, 4, and 8 weeks post intra-articular injection to evaluate the progression of histopathogenesis of osteoarthritis. Diacerein was orally administered (15 mg/kg) once daily post 1 and 2 weeks of iodoacetate injection in two groups, respectively, for up to 12 weeks. Articular cartilage and subchondral bone of the rats of both groups were examined after 8 and 12 weeks, respectively. Quantitative histological analyses were performed by scoring these sections as per the OARSI system. Chondroitin sulfate was also estimated in articular cartilage by decrease in absorbance of methylene blue on complexation with chondroitin sulfate using a spectrophotometer. Intra-articular injection of iodoacetate induced loss of articular cartilage with progressive subchondral bone sclerosis and degeneration. Based on histopathological and biochemical findings, diacerein treatment showed chondroprotective effect. Furthermore, the chondroprotective effect of diacerein was found to be more pronounced after 12 weeks as compared to 8 weeks in both cases (i.e., post 1 and 2 weeks of iodoacetate injection). Similar results were observed by investigation of chondroitin sulfate during biochemical study, showing the chondroprotective effect. In conclusion, diacerein exhibits chondroprotective effect in rats with late onset of action.

No MeSH data available.


Related in: MedlinePlus