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Endothelin-1-induced mini-stroke in the dorsal hippocampus or lateral amygdala results in deficits in learning and memory.

Sheng T, Zhang X, Wang S, Zhang J, Lu W, Dai Y - J Biomed Res (2015)

Bottom Line: In novel object task, ET-1 in the hippocampus also eliminated object identity memory.ET-1 in the lateral amygdale affected acquisition of fear conditioning and disrupted retention of tone-conditioned fear, but did not impair retention of contextual fear.These findings suggest that ET-1-induced mini-infarct in deep brain area leads to functional deficits in learning and memory associated with these regions.

View Article: PubMed Central - PubMed

Affiliation: The Center of Metabolic Disease Research.

ABSTRACT
Functional and structural alterations in brain connectivity associated with brain ischemia have been extensively studied. However, the mechanism whereby local ischemia in deep brain region affect brain functions is still unknown. Here, we first established a mini-stroke model by infusion of endothelin-1 (ET-1) into the dorsal hippocampus or the lateral amygdala, and then investigated how these mini-infarcts affected brain functions associated with these regions. We found that rats with ET-1 infusion showed deficit in recall of contextual fear memory, but not in learning process and recall of tone fear memory. In novel object task, ET-1 in the hippocampus also eliminated object identity memory. ET-1 in the lateral amygdale affected acquisition of fear conditioning and disrupted retention of tone-conditioned fear, but did not impair retention of contextual fear. These findings suggest that ET-1-induced mini-infarct in deep brain area leads to functional deficits in learning and memory associated with these regions.

No MeSH data available.


Related in: MedlinePlus

Rats with ET-1-induced DH mini-stroke showed normal spontaneous activity.A: Rats were injected with ET-1 in one side DH and saline in the other side DH. B: Representative T2-weighted MR imaging shows infarct injury of DH as a result of ET-1 injected into this position. C-F: Performance of the open-field task shows similar color-coded time-in-location map (C), the time in the central zone and surrounding zone (D), total moving distance (E) and average speed (F), indicating the same active mode for both treatment groups. In all the animals for behavioral test, ET-1 was injected bilaterally. In (C), same blue-to-red scale is used for each map.
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f01: Rats with ET-1-induced DH mini-stroke showed normal spontaneous activity.A: Rats were injected with ET-1 in one side DH and saline in the other side DH. B: Representative T2-weighted MR imaging shows infarct injury of DH as a result of ET-1 injected into this position. C-F: Performance of the open-field task shows similar color-coded time-in-location map (C), the time in the central zone and surrounding zone (D), total moving distance (E) and average speed (F), indicating the same active mode for both treatment groups. In all the animals for behavioral test, ET-1 was injected bilaterally. In (C), same blue-to-red scale is used for each map.

Mentions: To study ET-1 induced lesion in the hippocampus, we infused ET-1 into the dorsal hippocampus (Fig. 1A)[28].We used MRI to examine and confirm the establishment of the mini-stroke model. Twenty-four hours after injection, we used T2-weighted spin-echo MRI sequences to confirm the generation of mini-stroke (Fig. 1B)[25,29]. The site of local infarct shown by MRI matched ET-1 injection site in the dorsal hippocampus. In contrast to the recession of mobility caused by the damage that results from MCAO), rats receiving ET-1 showed normal spontaneous activity in open field test (Fig. 1C), moving distance (Fig. 1D) and average speed (Fig. 1E) compared to those receiving sham treatment.


Endothelin-1-induced mini-stroke in the dorsal hippocampus or lateral amygdala results in deficits in learning and memory.

Sheng T, Zhang X, Wang S, Zhang J, Lu W, Dai Y - J Biomed Res (2015)

Rats with ET-1-induced DH mini-stroke showed normal spontaneous activity.A: Rats were injected with ET-1 in one side DH and saline in the other side DH. B: Representative T2-weighted MR imaging shows infarct injury of DH as a result of ET-1 injected into this position. C-F: Performance of the open-field task shows similar color-coded time-in-location map (C), the time in the central zone and surrounding zone (D), total moving distance (E) and average speed (F), indicating the same active mode for both treatment groups. In all the animals for behavioral test, ET-1 was injected bilaterally. In (C), same blue-to-red scale is used for each map.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4585430&req=5

f01: Rats with ET-1-induced DH mini-stroke showed normal spontaneous activity.A: Rats were injected with ET-1 in one side DH and saline in the other side DH. B: Representative T2-weighted MR imaging shows infarct injury of DH as a result of ET-1 injected into this position. C-F: Performance of the open-field task shows similar color-coded time-in-location map (C), the time in the central zone and surrounding zone (D), total moving distance (E) and average speed (F), indicating the same active mode for both treatment groups. In all the animals for behavioral test, ET-1 was injected bilaterally. In (C), same blue-to-red scale is used for each map.
Mentions: To study ET-1 induced lesion in the hippocampus, we infused ET-1 into the dorsal hippocampus (Fig. 1A)[28].We used MRI to examine and confirm the establishment of the mini-stroke model. Twenty-four hours after injection, we used T2-weighted spin-echo MRI sequences to confirm the generation of mini-stroke (Fig. 1B)[25,29]. The site of local infarct shown by MRI matched ET-1 injection site in the dorsal hippocampus. In contrast to the recession of mobility caused by the damage that results from MCAO), rats receiving ET-1 showed normal spontaneous activity in open field test (Fig. 1C), moving distance (Fig. 1D) and average speed (Fig. 1E) compared to those receiving sham treatment.

Bottom Line: In novel object task, ET-1 in the hippocampus also eliminated object identity memory.ET-1 in the lateral amygdale affected acquisition of fear conditioning and disrupted retention of tone-conditioned fear, but did not impair retention of contextual fear.These findings suggest that ET-1-induced mini-infarct in deep brain area leads to functional deficits in learning and memory associated with these regions.

View Article: PubMed Central - PubMed

Affiliation: The Center of Metabolic Disease Research.

ABSTRACT
Functional and structural alterations in brain connectivity associated with brain ischemia have been extensively studied. However, the mechanism whereby local ischemia in deep brain region affect brain functions is still unknown. Here, we first established a mini-stroke model by infusion of endothelin-1 (ET-1) into the dorsal hippocampus or the lateral amygdala, and then investigated how these mini-infarcts affected brain functions associated with these regions. We found that rats with ET-1 infusion showed deficit in recall of contextual fear memory, but not in learning process and recall of tone fear memory. In novel object task, ET-1 in the hippocampus also eliminated object identity memory. ET-1 in the lateral amygdale affected acquisition of fear conditioning and disrupted retention of tone-conditioned fear, but did not impair retention of contextual fear. These findings suggest that ET-1-induced mini-infarct in deep brain area leads to functional deficits in learning and memory associated with these regions.

No MeSH data available.


Related in: MedlinePlus