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Simultaneous effects of leaf irradiance and soil moisture on growth and root system architecture of novel wheat genotypes: implications for phenotyping.

Nagel KA, Bonnett D, Furbank R, Walter A, Schurr U, Watt M - J. Exp. Bot. (2015)

Bottom Line: In contrast, the other genotype showed much less plasticity and responsiveness to upper moist soil, but maintained deeper penetration of roots into the dry layer.The sum of shoot and root responses was greater when treated simultaneously to low light and low soil water, compared to each treatment alone, suggesting the value of whole plant phenotyping in response to multiple conditions for agronomic improvement.The results suggest that canopy management for increased irradiation of leaves would encourage root growth into deeper drier soil, and that genetic variation within closely related breeding lines may exist to favour surface root growth in response to irrigation or in-season rainfall.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany k.nagel@fz-juelich.de.

No MeSH data available.


Related in: MedlinePlus

Ratio between visible and non-visible root system of wheat plants grown in soil-filled rhizoboxes. Root length visible at the transparent surface of the rhizoboxes is plotted against the total root length after harvesting the plants.
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Figure 2: Ratio between visible and non-visible root system of wheat plants grown in soil-filled rhizoboxes. Root length visible at the transparent surface of the rhizoboxes is plotted against the total root length after harvesting the plants.

Mentions: Three times per week, the number of main stem leaves and tiller leaves was counted, and the length and width of all leaves measured with a ruler. The measurement was started 10 days after sowing, when the first leaf was unrolled. In total, the leaves were measured at five time points. The total leaf area (A) was then calculated according to A = leaf width × leaf length × 0.858 (Kalra and Dhiman, 1977). The measurement of roots started 5 days after sowing, when the first roots were visible at the transparent surface of the rhizoboxes. In total, the roots were measured at seven time points. Three times per week, the number of lateral roots arising from the primary seminal roots was counted, and the root depth measured as the vertical distance between the seed and the deepest root tip. The total root length (sum of seminal and lateral root length) was measured non-invasively by tracing the roots visible at the transparent surface of the rhizobox. The roots were first traced on transparency film, and the root length was then determined by scanning the film and analysing with WinRhizo software (WinRhizo, Regent Instrument). The visible root length at the surface of the rhizobox represented part of the total root system length. To establish the effect of different light and soil moisture treatments on all of the root length, it was necessary to define the relationship between visible and non-visible roots. To do this, plants were harvested, roots washed out, and root length determined with WinRhizo. The visible root length represented approximately 30% of the total root system length, which is consistent with published data about the root fraction growing on the glass face of rhizoboxes (Hurd, 1963; Nagel et al., 2012). The root length visible at the rhizobox surface and the total root length (visible and non-visible roots) showed a strong correlation (R2 = 0.91, Fig. 2). Hence, the non-destructive analysis of the root length at the rhizobox surface is a reliable measure of the total root length, and the effects of shoot and root treatments.


Simultaneous effects of leaf irradiance and soil moisture on growth and root system architecture of novel wheat genotypes: implications for phenotyping.

Nagel KA, Bonnett D, Furbank R, Walter A, Schurr U, Watt M - J. Exp. Bot. (2015)

Ratio between visible and non-visible root system of wheat plants grown in soil-filled rhizoboxes. Root length visible at the transparent surface of the rhizoboxes is plotted against the total root length after harvesting the plants.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585422&req=5

Figure 2: Ratio between visible and non-visible root system of wheat plants grown in soil-filled rhizoboxes. Root length visible at the transparent surface of the rhizoboxes is plotted against the total root length after harvesting the plants.
Mentions: Three times per week, the number of main stem leaves and tiller leaves was counted, and the length and width of all leaves measured with a ruler. The measurement was started 10 days after sowing, when the first leaf was unrolled. In total, the leaves were measured at five time points. The total leaf area (A) was then calculated according to A = leaf width × leaf length × 0.858 (Kalra and Dhiman, 1977). The measurement of roots started 5 days after sowing, when the first roots were visible at the transparent surface of the rhizoboxes. In total, the roots were measured at seven time points. Three times per week, the number of lateral roots arising from the primary seminal roots was counted, and the root depth measured as the vertical distance between the seed and the deepest root tip. The total root length (sum of seminal and lateral root length) was measured non-invasively by tracing the roots visible at the transparent surface of the rhizobox. The roots were first traced on transparency film, and the root length was then determined by scanning the film and analysing with WinRhizo software (WinRhizo, Regent Instrument). The visible root length at the surface of the rhizobox represented part of the total root system length. To establish the effect of different light and soil moisture treatments on all of the root length, it was necessary to define the relationship between visible and non-visible roots. To do this, plants were harvested, roots washed out, and root length determined with WinRhizo. The visible root length represented approximately 30% of the total root system length, which is consistent with published data about the root fraction growing on the glass face of rhizoboxes (Hurd, 1963; Nagel et al., 2012). The root length visible at the rhizobox surface and the total root length (visible and non-visible roots) showed a strong correlation (R2 = 0.91, Fig. 2). Hence, the non-destructive analysis of the root length at the rhizobox surface is a reliable measure of the total root length, and the effects of shoot and root treatments.

Bottom Line: In contrast, the other genotype showed much less plasticity and responsiveness to upper moist soil, but maintained deeper penetration of roots into the dry layer.The sum of shoot and root responses was greater when treated simultaneously to low light and low soil water, compared to each treatment alone, suggesting the value of whole plant phenotyping in response to multiple conditions for agronomic improvement.The results suggest that canopy management for increased irradiation of leaves would encourage root growth into deeper drier soil, and that genetic variation within closely related breeding lines may exist to favour surface root growth in response to irrigation or in-season rainfall.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany k.nagel@fz-juelich.de.

No MeSH data available.


Related in: MedlinePlus