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Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves.

Tubuxin B, Rahimzadeh-Bajgiran P, Ginnan Y, Hosoi F, Omasa K - J. Exp. Bot. (2015)

Bottom Line: The steady-state solar-induced Chl fluorescence yield ratio correlated very well with the artificial-light-induced one (R(2) = 0.84).A new methodology is then presented to estimate photochemical yield of photosystem II (ΦPSII) from the SIF measurements, which was verified against the standard Chl fluorescence measurement method (pulse-amplitude modulated method).The high coefficient of determination (R(2) = 0.74) between the ΦPSII of the two methods shows that photosynthesis process parameters can be successfully estimated using the presented methodology.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.

No MeSH data available.


Measurement of Chl fluorescence using the FLD method under solar light and saturation pulse for estimating ΦPSII of paprika leaf. (A) Temporal changes in leaf radiant intensity spectra measured using a HR2000+ spectrometer under solar light (1450 μmol m−2 s−1), where SLRL is the saturation light pulse (4450 μmol m−2 s−1) using red laser (660nm) and SLPAM is the saturation light pulse (blue LED, about 10 000 μmol m−2 s−1) of JUNIOR PAM. The solid line is the spectra of 758.9nm and the dotted line is that of 760.4nm. A mean value of 0.4nm wavelength width (W) was used. (B) Temporal changes in Chl fluorescence intensity (F760.4) calculated by the FLD method. (C) Temporal changes in Chl fluorescence yield (ΦF760.4). The dotted line is the changes in ΦF760.4 and the solid line is the value after median filtering during the 10 s before the SLRL illumination for estimating ΦPSII.
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Figure 7: Measurement of Chl fluorescence using the FLD method under solar light and saturation pulse for estimating ΦPSII of paprika leaf. (A) Temporal changes in leaf radiant intensity spectra measured using a HR2000+ spectrometer under solar light (1450 μmol m−2 s−1), where SLRL is the saturation light pulse (4450 μmol m−2 s−1) using red laser (660nm) and SLPAM is the saturation light pulse (blue LED, about 10 000 μmol m−2 s−1) of JUNIOR PAM. The solid line is the spectra of 758.9nm and the dotted line is that of 760.4nm. A mean value of 0.4nm wavelength width (W) was used. (B) Temporal changes in Chl fluorescence intensity (F760.4) calculated by the FLD method. (C) Temporal changes in Chl fluorescence yield (ΦF760.4). The dotted line is the changes in ΦF760.4 and the solid line is the value after median filtering during the 10 s before the SLRL illumination for estimating ΦPSII.

Mentions: Figure 7 shows an example of Chl fluorescence measurement using the FLD method under solar light and saturation pulse for estimating ΦPSII of the attached leaf. The temporal changes in radiant intensity measurement of (ii) to (vi) mentioned in Materials and methods, Spectral radiant intensity measurement for ΦPSII estimation under solar light, are shown in Fig. 7A. The radiant intensity increased with the saturation pulses of 660nm laser (SLRL) and blue LED (SLPAM) of JUNIOR PAM. Fig. 7B, C shows temporal changes in Chl fluorescence intensity (F760.4) calculated by the FLD method and those in Chl fluorescence yield (ΦF760.4) calculated by equation (3). The solid line in Fig. 7C is the sunlight fluorescence yield (ΦF) without noise after median filtering during 10 s before the SLRL illumination for estimating ΦPSII. The saturation laser-induced fluorescence yield (ΦFm’) under solar light was larger than the yield induced by SLPAM. This result might have been caused by the difference in beam footprint sizes between the HR2000+ spectrometer and JUNIOR PAM.


Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves.

Tubuxin B, Rahimzadeh-Bajgiran P, Ginnan Y, Hosoi F, Omasa K - J. Exp. Bot. (2015)

Measurement of Chl fluorescence using the FLD method under solar light and saturation pulse for estimating ΦPSII of paprika leaf. (A) Temporal changes in leaf radiant intensity spectra measured using a HR2000+ spectrometer under solar light (1450 μmol m−2 s−1), where SLRL is the saturation light pulse (4450 μmol m−2 s−1) using red laser (660nm) and SLPAM is the saturation light pulse (blue LED, about 10 000 μmol m−2 s−1) of JUNIOR PAM. The solid line is the spectra of 758.9nm and the dotted line is that of 760.4nm. A mean value of 0.4nm wavelength width (W) was used. (B) Temporal changes in Chl fluorescence intensity (F760.4) calculated by the FLD method. (C) Temporal changes in Chl fluorescence yield (ΦF760.4). The dotted line is the changes in ΦF760.4 and the solid line is the value after median filtering during the 10 s before the SLRL illumination for estimating ΦPSII.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 7: Measurement of Chl fluorescence using the FLD method under solar light and saturation pulse for estimating ΦPSII of paprika leaf. (A) Temporal changes in leaf radiant intensity spectra measured using a HR2000+ spectrometer under solar light (1450 μmol m−2 s−1), where SLRL is the saturation light pulse (4450 μmol m−2 s−1) using red laser (660nm) and SLPAM is the saturation light pulse (blue LED, about 10 000 μmol m−2 s−1) of JUNIOR PAM. The solid line is the spectra of 758.9nm and the dotted line is that of 760.4nm. A mean value of 0.4nm wavelength width (W) was used. (B) Temporal changes in Chl fluorescence intensity (F760.4) calculated by the FLD method. (C) Temporal changes in Chl fluorescence yield (ΦF760.4). The dotted line is the changes in ΦF760.4 and the solid line is the value after median filtering during the 10 s before the SLRL illumination for estimating ΦPSII.
Mentions: Figure 7 shows an example of Chl fluorescence measurement using the FLD method under solar light and saturation pulse for estimating ΦPSII of the attached leaf. The temporal changes in radiant intensity measurement of (ii) to (vi) mentioned in Materials and methods, Spectral radiant intensity measurement for ΦPSII estimation under solar light, are shown in Fig. 7A. The radiant intensity increased with the saturation pulses of 660nm laser (SLRL) and blue LED (SLPAM) of JUNIOR PAM. Fig. 7B, C shows temporal changes in Chl fluorescence intensity (F760.4) calculated by the FLD method and those in Chl fluorescence yield (ΦF760.4) calculated by equation (3). The solid line in Fig. 7C is the sunlight fluorescence yield (ΦF) without noise after median filtering during 10 s before the SLRL illumination for estimating ΦPSII. The saturation laser-induced fluorescence yield (ΦFm’) under solar light was larger than the yield induced by SLPAM. This result might have been caused by the difference in beam footprint sizes between the HR2000+ spectrometer and JUNIOR PAM.

Bottom Line: The steady-state solar-induced Chl fluorescence yield ratio correlated very well with the artificial-light-induced one (R(2) = 0.84).A new methodology is then presented to estimate photochemical yield of photosystem II (ΦPSII) from the SIF measurements, which was verified against the standard Chl fluorescence measurement method (pulse-amplitude modulated method).The high coefficient of determination (R(2) = 0.74) between the ΦPSII of the two methods shows that photosynthesis process parameters can be successfully estimated using the presented methodology.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.

No MeSH data available.