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Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves.

Tubuxin B, Rahimzadeh-Bajgiran P, Ginnan Y, Hosoi F, Omasa K - J. Exp. Bot. (2015)

Bottom Line: The steady-state solar-induced Chl fluorescence yield ratio correlated very well with the artificial-light-induced one (R(2) = 0.84).A new methodology is then presented to estimate photochemical yield of photosystem II (ΦPSII) from the SIF measurements, which was verified against the standard Chl fluorescence measurement method (pulse-amplitude modulated method).The high coefficient of determination (R(2) = 0.74) between the ΦPSII of the two methods shows that photosynthesis process parameters can be successfully estimated using the presented methodology.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.

No MeSH data available.


Steady-state Chl fluorescence emission spectra and absorption spectra of different Chl content leaves taken under artificial illumination (λ < 600nm). (A) The steady-state Chl fluorescence emission spectra. (B) Absorptance spectra of the same leaf sample. Chl content: the solid line is 502.3mg m−2, the dashed line is 160.8mg m−2, and the dotted line is 62.9mg m−2.
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Figure 2: Steady-state Chl fluorescence emission spectra and absorption spectra of different Chl content leaves taken under artificial illumination (λ < 600nm). (A) The steady-state Chl fluorescence emission spectra. (B) Absorptance spectra of the same leaf sample. Chl content: the solid line is 502.3mg m−2, the dashed line is 160.8mg m−2, and the dotted line is 62.9mg m−2.

Mentions: Figure 2A shows the steady-state Chl fluorescence emission spectrum of three leaves with different Chl content taken under the artificial illumination light (λ < 600nm). Two maxima appeared in the red region around 686nm (F686) and far-red region around 740nm (F740). Near the red region, Chl fluorescence increased sharply with the reduction of Chl content, but near the far-red region, it increased with the increase of Chl content. The Chl absorptance near 686nm greatly increased with increasing Chl content, whereas there was little difference near 760nm (Fig. 2B).


Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves.

Tubuxin B, Rahimzadeh-Bajgiran P, Ginnan Y, Hosoi F, Omasa K - J. Exp. Bot. (2015)

Steady-state Chl fluorescence emission spectra and absorption spectra of different Chl content leaves taken under artificial illumination (λ < 600nm). (A) The steady-state Chl fluorescence emission spectra. (B) Absorptance spectra of the same leaf sample. Chl content: the solid line is 502.3mg m−2, the dashed line is 160.8mg m−2, and the dotted line is 62.9mg m−2.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585421&req=5

Figure 2: Steady-state Chl fluorescence emission spectra and absorption spectra of different Chl content leaves taken under artificial illumination (λ < 600nm). (A) The steady-state Chl fluorescence emission spectra. (B) Absorptance spectra of the same leaf sample. Chl content: the solid line is 502.3mg m−2, the dashed line is 160.8mg m−2, and the dotted line is 62.9mg m−2.
Mentions: Figure 2A shows the steady-state Chl fluorescence emission spectrum of three leaves with different Chl content taken under the artificial illumination light (λ < 600nm). Two maxima appeared in the red region around 686nm (F686) and far-red region around 740nm (F740). Near the red region, Chl fluorescence increased sharply with the reduction of Chl content, but near the far-red region, it increased with the increase of Chl content. The Chl absorptance near 686nm greatly increased with increasing Chl content, whereas there was little difference near 760nm (Fig. 2B).

Bottom Line: The steady-state solar-induced Chl fluorescence yield ratio correlated very well with the artificial-light-induced one (R(2) = 0.84).A new methodology is then presented to estimate photochemical yield of photosystem II (ΦPSII) from the SIF measurements, which was verified against the standard Chl fluorescence measurement method (pulse-amplitude modulated method).The high coefficient of determination (R(2) = 0.74) between the ΦPSII of the two methods shows that photosynthesis process parameters can be successfully estimated using the presented methodology.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.

No MeSH data available.