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Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves.

Tubuxin B, Rahimzadeh-Bajgiran P, Ginnan Y, Hosoi F, Omasa K - J. Exp. Bot. (2015)

Bottom Line: The steady-state solar-induced Chl fluorescence yield ratio correlated very well with the artificial-light-induced one (R(2) = 0.84).A new methodology is then presented to estimate photochemical yield of photosystem II (ΦPSII) from the SIF measurements, which was verified against the standard Chl fluorescence measurement method (pulse-amplitude modulated method).The high coefficient of determination (R(2) = 0.74) between the ΦPSII of the two methods shows that photosynthesis process parameters can be successfully estimated using the presented methodology.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.

No MeSH data available.


Concept of Fraunhofer line depth (FLD) method. (A) O2B band. (B) O2A band. The solid line is non-fluorescent reference radiant intensity spectra and the dashed line is paprika (Capsicum annuum cv. ‘Sven’) leaf radiant intensity spectra measured in O2A and O2B bands. The bands a and c are the border of the well and the bands b and d are the bottom of the well for the reflectance and leaf. The W is wavelength width in band a, b, c, and d. The measured value of radiant intensity is shown as the digital number (DN) and is not calibrated to exact radiance. The leaf Chl content was 621.1mg m−2.
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Figure 1: Concept of Fraunhofer line depth (FLD) method. (A) O2B band. (B) O2A band. The solid line is non-fluorescent reference radiant intensity spectra and the dashed line is paprika (Capsicum annuum cv. ‘Sven’) leaf radiant intensity spectra measured in O2A and O2B bands. The bands a and c are the border of the well and the bands b and d are the bottom of the well for the reflectance and leaf. The W is wavelength width in band a, b, c, and d. The measured value of radiant intensity is shown as the digital number (DN) and is not calibrated to exact radiance. The leaf Chl content was 621.1mg m−2.

Mentions: Fig. 1 shows the FLD method. In Fig. 1A, B, the solid line is the non-fluorescent reference radiant intensity spectra and the dashed line is the leaf radiant intensity spectra measured in O2A and O2B bands. Ma and Mb are the mean values of radiant intensity (digital numbers, which is not calibrated to exact radiance) from the non-fluorescent reference in band a (border of the well, A: W = 685.93 to 686.32nm; B: W = 758.76 to 759.17nm; W: the wavelength width) and in band b (bottom of the well, A: W = 686.54 to 686.93nm; B: W = 760.24 to 760.64nm), and Mc and Md are the mean values of radiant intensity from the leaf in band c (the same W as a) and in band d (the same W as b), respectively. Following Plascyk and Gabriel (1975) and Moya et al. (2004), the reflectance coefficient (R) and the solar-induced fluorescence intensity (Fs) were derived from the measured values of Ma, Mb, Mc and Md as follows:


Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves.

Tubuxin B, Rahimzadeh-Bajgiran P, Ginnan Y, Hosoi F, Omasa K - J. Exp. Bot. (2015)

Concept of Fraunhofer line depth (FLD) method. (A) O2B band. (B) O2A band. The solid line is non-fluorescent reference radiant intensity spectra and the dashed line is paprika (Capsicum annuum cv. ‘Sven’) leaf radiant intensity spectra measured in O2A and O2B bands. The bands a and c are the border of the well and the bands b and d are the bottom of the well for the reflectance and leaf. The W is wavelength width in band a, b, c, and d. The measured value of radiant intensity is shown as the digital number (DN) and is not calibrated to exact radiance. The leaf Chl content was 621.1mg m−2.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585421&req=5

Figure 1: Concept of Fraunhofer line depth (FLD) method. (A) O2B band. (B) O2A band. The solid line is non-fluorescent reference radiant intensity spectra and the dashed line is paprika (Capsicum annuum cv. ‘Sven’) leaf radiant intensity spectra measured in O2A and O2B bands. The bands a and c are the border of the well and the bands b and d are the bottom of the well for the reflectance and leaf. The W is wavelength width in band a, b, c, and d. The measured value of radiant intensity is shown as the digital number (DN) and is not calibrated to exact radiance. The leaf Chl content was 621.1mg m−2.
Mentions: Fig. 1 shows the FLD method. In Fig. 1A, B, the solid line is the non-fluorescent reference radiant intensity spectra and the dashed line is the leaf radiant intensity spectra measured in O2A and O2B bands. Ma and Mb are the mean values of radiant intensity (digital numbers, which is not calibrated to exact radiance) from the non-fluorescent reference in band a (border of the well, A: W = 685.93 to 686.32nm; B: W = 758.76 to 759.17nm; W: the wavelength width) and in band b (bottom of the well, A: W = 686.54 to 686.93nm; B: W = 760.24 to 760.64nm), and Mc and Md are the mean values of radiant intensity from the leaf in band c (the same W as a) and in band d (the same W as b), respectively. Following Plascyk and Gabriel (1975) and Moya et al. (2004), the reflectance coefficient (R) and the solar-induced fluorescence intensity (Fs) were derived from the measured values of Ma, Mb, Mc and Md as follows:

Bottom Line: The steady-state solar-induced Chl fluorescence yield ratio correlated very well with the artificial-light-induced one (R(2) = 0.84).A new methodology is then presented to estimate photochemical yield of photosystem II (ΦPSII) from the SIF measurements, which was verified against the standard Chl fluorescence measurement method (pulse-amplitude modulated method).The high coefficient of determination (R(2) = 0.74) between the ΦPSII of the two methods shows that photosynthesis process parameters can be successfully estimated using the presented methodology.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.

No MeSH data available.