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LeasyScan: a novel concept combining 3D imaging and lysimetry for high-throughput phenotyping of traits controlling plant water budget.

Vadez V, Kholová J, Hummel G, Zhokhavets U, Gupta SK, Hash CT - J. Exp. Bot. (2015)

Bottom Line: Close agreement between scanned and observed leaf area data of individual plants in different crops was found (R(2) between 0.86 and 0.94).Similar agreement was found when comparing scanned and observed area of plants cultivated at densities reflecting field conditions (R(2) between 0.80 and 0.96).This new platform has the potential to phenotype for traits controlling plant water use at a high rate and precision, of critical importance for drought adaptation, and creates an opportunity to harness their genetics for the breeding of improved varieties.

View Article: PubMed Central - PubMed

Affiliation: ICRISAT-Crop Physiology Laboratory, Greater Hyderabad, Patancheru 502324, Telangana, India v.vadez@cgiar.org.

No MeSH data available.


3D leaf area development dynamics within a 12 d period covering the 155–273 degree-days thermal time in (A) pearl millet hybrids and (B) B-lines, adapted to different agroecological zones of India ((A1, rainfall <300–400mm; A, rainfall >400mm in Northern states of India; B, rainfall >400mm for Peninsular states of India). Each data point for the hybrids is the mean (±SE) of 14, 13 and 13 hybrids for the A1, A, and B zone, respectively, and of 4, 8 and 5 B-lines for the A1, A and B zone, respectively.
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Figure 8: 3D leaf area development dynamics within a 12 d period covering the 155–273 degree-days thermal time in (A) pearl millet hybrids and (B) B-lines, adapted to different agroecological zones of India ((A1, rainfall <300–400mm; A, rainfall >400mm in Northern states of India; B, rainfall >400mm for Peninsular states of India). Each data point for the hybrids is the mean (±SE) of 14, 13 and 13 hybrids for the A1, A, and B zone, respectively, and of 4, 8 and 5 B-lines for the A1, A and B zone, respectively.

Mentions: Figure 8A shows that the leaf area development pattern of F1 hybrids bred for the A1 zone was dramatically different from those of F1 hybrids developed for either the A or the B zone. There were no significant difference between the leaf area development pattern of A and B zones. The range of variation, proxied by the size of the variation from the mean, in the A1 hybrids also shows that the variation among hybrids bred for the same zone was larger than for hybrids bred for the A and B zone. Even larger zone differences were found in the leaf area development pattern between B-lines bred for the A1 zone and those bred for the A and B zones. Similar but less striking variation was found for the R-lines (data not shown). Therefore, it appears clearly that materials bred for the A1 zones developed smaller leaf area as earlier discussed (van Oosterom et al., 2003), in the order of 15% less for the F1 hybrids and in the order of 40% less for the B-lines. Early maturing hybrids (65–70 days to maturity) targeted for drought prone environments of the A1 zone indeed produce lower biomass in comparison to medium to late maturing hybrids (75–85 days to maturity) bred for relatively wetter A and B zones, hence the lesser leaf area in A1 hybrids was as expected.


LeasyScan: a novel concept combining 3D imaging and lysimetry for high-throughput phenotyping of traits controlling plant water budget.

Vadez V, Kholová J, Hummel G, Zhokhavets U, Gupta SK, Hash CT - J. Exp. Bot. (2015)

3D leaf area development dynamics within a 12 d period covering the 155–273 degree-days thermal time in (A) pearl millet hybrids and (B) B-lines, adapted to different agroecological zones of India ((A1, rainfall <300–400mm; A, rainfall >400mm in Northern states of India; B, rainfall >400mm for Peninsular states of India). Each data point for the hybrids is the mean (±SE) of 14, 13 and 13 hybrids for the A1, A, and B zone, respectively, and of 4, 8 and 5 B-lines for the A1, A and B zone, respectively.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585418&req=5

Figure 8: 3D leaf area development dynamics within a 12 d period covering the 155–273 degree-days thermal time in (A) pearl millet hybrids and (B) B-lines, adapted to different agroecological zones of India ((A1, rainfall <300–400mm; A, rainfall >400mm in Northern states of India; B, rainfall >400mm for Peninsular states of India). Each data point for the hybrids is the mean (±SE) of 14, 13 and 13 hybrids for the A1, A, and B zone, respectively, and of 4, 8 and 5 B-lines for the A1, A and B zone, respectively.
Mentions: Figure 8A shows that the leaf area development pattern of F1 hybrids bred for the A1 zone was dramatically different from those of F1 hybrids developed for either the A or the B zone. There were no significant difference between the leaf area development pattern of A and B zones. The range of variation, proxied by the size of the variation from the mean, in the A1 hybrids also shows that the variation among hybrids bred for the same zone was larger than for hybrids bred for the A and B zone. Even larger zone differences were found in the leaf area development pattern between B-lines bred for the A1 zone and those bred for the A and B zones. Similar but less striking variation was found for the R-lines (data not shown). Therefore, it appears clearly that materials bred for the A1 zones developed smaller leaf area as earlier discussed (van Oosterom et al., 2003), in the order of 15% less for the F1 hybrids and in the order of 40% less for the B-lines. Early maturing hybrids (65–70 days to maturity) targeted for drought prone environments of the A1 zone indeed produce lower biomass in comparison to medium to late maturing hybrids (75–85 days to maturity) bred for relatively wetter A and B zones, hence the lesser leaf area in A1 hybrids was as expected.

Bottom Line: Close agreement between scanned and observed leaf area data of individual plants in different crops was found (R(2) between 0.86 and 0.94).Similar agreement was found when comparing scanned and observed area of plants cultivated at densities reflecting field conditions (R(2) between 0.80 and 0.96).This new platform has the potential to phenotype for traits controlling plant water use at a high rate and precision, of critical importance for drought adaptation, and creates an opportunity to harness their genetics for the breeding of improved varieties.

View Article: PubMed Central - PubMed

Affiliation: ICRISAT-Crop Physiology Laboratory, Greater Hyderabad, Patancheru 502324, Telangana, India v.vadez@cgiar.org.

No MeSH data available.