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LeasyScan: a novel concept combining 3D imaging and lysimetry for high-throughput phenotyping of traits controlling plant water budget.

Vadez V, Kholová J, Hummel G, Zhokhavets U, Gupta SK, Hash CT - J. Exp. Bot. (2015)

Bottom Line: Close agreement between scanned and observed leaf area data of individual plants in different crops was found (R(2) between 0.86 and 0.94).Similar agreement was found when comparing scanned and observed area of plants cultivated at densities reflecting field conditions (R(2) between 0.80 and 0.96).This new platform has the potential to phenotype for traits controlling plant water use at a high rate and precision, of critical importance for drought adaptation, and creates an opportunity to harness their genetics for the breeding of improved varieties.

View Article: PubMed Central - PubMed

Affiliation: ICRISAT-Crop Physiology Laboratory, Greater Hyderabad, Patancheru 502324, Telangana, India v.vadez@cgiar.org.

No MeSH data available.


Related in: MedlinePlus

Phenotyping platform—LeasyScan. Scanner support device with eight scanners (PlantEyeR) can assess the information from 3200/4800 sectors (60×60/60×40cm – a sector represents a replication unit and encompasses two pots) in 2h intervals with standard speed 50mm s-1; The platform length is 129.5 m and allows assessment of plant height, canopy size (3D area, projected leaf area) and canopy structure parameters (leaf angle).
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Figure 2: Phenotyping platform—LeasyScan. Scanner support device with eight scanners (PlantEyeR) can assess the information from 3200/4800 sectors (60×60/60×40cm – a sector represents a replication unit and encompasses two pots) in 2h intervals with standard speed 50mm s-1; The platform length is 129.5 m and allows assessment of plant height, canopy size (3D area, projected leaf area) and canopy structure parameters (leaf angle).

Mentions: The scanners are preset to image an area of 65cm width and a length of either 40 or 60cm, which is called a ‘sector’. The volume in which the 3D image is generated is then a cuboid of 65×40×100cm or 65×60×100cm. While extracting parameter data from the 3D image, the scanners are set to ignore point clouds that are below a certain height (pot height). Sector-wise binning of data point clouds is performed using a system of barcodes every 5 m to reset the scanner position in height and length. As in the lysimetric facility (Vadez et al., 2014), our deliberate choice was to remain as close as possible to the field conditions and consider each of these sectors as a plot, in which plants are cultivated in each sector at a density similar to the field (for instance 24–32 plants per square metre for chickpea or 16 plants per square metre for pearl millet or sorghum). Therefore, each sector represents an experimental unit, and in the rest of the manuscript sectors may be called as such, or plots, or experimental units. The scanners are mounted on top of an irrigation boom, which is electronically controlled to be fully automated and speed-controlled. At a movement speed of 3 m min-1, eight scanners are capable of scanning 4800 sectors in slightly less than 2h. The speed and frequency of scanning can be adjusted depending on necessity—we currently operate at a rate of 12 scans per sector per day. The plant parameters that are measured by the scanners are the total leaf area (which is called 3D-leaf area), projected area (which is akin to the leaf area index, LAI), leaf angle (i.e. the average angle of vectors perpendicular to the surface of each triangulated unit, also called surface normal) and plant height (Fig. 2, the platform).


LeasyScan: a novel concept combining 3D imaging and lysimetry for high-throughput phenotyping of traits controlling plant water budget.

Vadez V, Kholová J, Hummel G, Zhokhavets U, Gupta SK, Hash CT - J. Exp. Bot. (2015)

Phenotyping platform—LeasyScan. Scanner support device with eight scanners (PlantEyeR) can assess the information from 3200/4800 sectors (60×60/60×40cm – a sector represents a replication unit and encompasses two pots) in 2h intervals with standard speed 50mm s-1; The platform length is 129.5 m and allows assessment of plant height, canopy size (3D area, projected leaf area) and canopy structure parameters (leaf angle).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585418&req=5

Figure 2: Phenotyping platform—LeasyScan. Scanner support device with eight scanners (PlantEyeR) can assess the information from 3200/4800 sectors (60×60/60×40cm – a sector represents a replication unit and encompasses two pots) in 2h intervals with standard speed 50mm s-1; The platform length is 129.5 m and allows assessment of plant height, canopy size (3D area, projected leaf area) and canopy structure parameters (leaf angle).
Mentions: The scanners are preset to image an area of 65cm width and a length of either 40 or 60cm, which is called a ‘sector’. The volume in which the 3D image is generated is then a cuboid of 65×40×100cm or 65×60×100cm. While extracting parameter data from the 3D image, the scanners are set to ignore point clouds that are below a certain height (pot height). Sector-wise binning of data point clouds is performed using a system of barcodes every 5 m to reset the scanner position in height and length. As in the lysimetric facility (Vadez et al., 2014), our deliberate choice was to remain as close as possible to the field conditions and consider each of these sectors as a plot, in which plants are cultivated in each sector at a density similar to the field (for instance 24–32 plants per square metre for chickpea or 16 plants per square metre for pearl millet or sorghum). Therefore, each sector represents an experimental unit, and in the rest of the manuscript sectors may be called as such, or plots, or experimental units. The scanners are mounted on top of an irrigation boom, which is electronically controlled to be fully automated and speed-controlled. At a movement speed of 3 m min-1, eight scanners are capable of scanning 4800 sectors in slightly less than 2h. The speed and frequency of scanning can be adjusted depending on necessity—we currently operate at a rate of 12 scans per sector per day. The plant parameters that are measured by the scanners are the total leaf area (which is called 3D-leaf area), projected area (which is akin to the leaf area index, LAI), leaf angle (i.e. the average angle of vectors perpendicular to the surface of each triangulated unit, also called surface normal) and plant height (Fig. 2, the platform).

Bottom Line: Close agreement between scanned and observed leaf area data of individual plants in different crops was found (R(2) between 0.86 and 0.94).Similar agreement was found when comparing scanned and observed area of plants cultivated at densities reflecting field conditions (R(2) between 0.80 and 0.96).This new platform has the potential to phenotype for traits controlling plant water use at a high rate and precision, of critical importance for drought adaptation, and creates an opportunity to harness their genetics for the breeding of improved varieties.

View Article: PubMed Central - PubMed

Affiliation: ICRISAT-Crop Physiology Laboratory, Greater Hyderabad, Patancheru 502324, Telangana, India v.vadez@cgiar.org.

No MeSH data available.


Related in: MedlinePlus