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Characterizing root response phenotypes by neural network analysis.

Hatzig SV, Schiessl S, Stahl A, Snowdon RJ - J. Exp. Bot. (2015)

Bottom Line: Interactive changes in root architecture can be easily captured by individual intersection profiles generated by Sholl analysis.Validation using manual measurements confirmed that the number of lateral roots decreased, while mean lateral root length was enhanced, under osmotic stress conditions.The Sholl methodology is presented as a promising tool for selection of cultivars with advantageous root phenotypes under osmotic stress conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany sarah.hatzig@agrar.uni-giessen.de.

No MeSH data available.


Number of root intersections depending on the distance from root origin of a DR (dotted lines) and a DS (squared lines) winter oilseed rape genotype, measured under control conditions (open symbols) and after 5 days of PEG 6000 treatment (closed symbols). Points are means of five replicates with standard errors (this figure is available in colour at JXB online).
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Figure 4: Number of root intersections depending on the distance from root origin of a DR (dotted lines) and a DS (squared lines) winter oilseed rape genotype, measured under control conditions (open symbols) and after 5 days of PEG 6000 treatment (closed symbols). Points are means of five replicates with standard errors (this figure is available in colour at JXB online).

Mentions: No difference in absolute number of root-circle intersections was observed between the control treatments of the two investigated B. napus genotypes (Fig. 3). Under osmotic stress conditions, however, genotype DR reacted with a significant increase in intersection number, while for genotype DS no difference in intersection number could be observed between treatments. In genotype DR the absolute number of intersections increased from 173 (control) to 258 (PEG 6000), while DS showed 165 intersections under control conditions and 163 after PEG 6000 treatment. Under control conditions the last root-circle intersection in genotype DR occurred at a radius of 26cm, whereas for genotype DS the farthest circle intersection was measured at 21cm from the root origin (Fig. 4).Outermost root intersection under PEG 6000 treatment was measured at radii of 22cm and 18cm for DR and DS, respectively. Under control conditions, both genotypes showed a similar number of root-circle intersections between 0.5 and 4cm distance to the root origin. The number of root intersections was significantly lower at 6.5cm for genotype DR than for DS, whereas it was significantly higher at most circles between 9.5 and 26cm from the origin. Both genotypes showed a significant circle-specific increase in intersection number in the PEG 6000 treatment. In DR the intersection number increased significantly between 3 and 10cm, whereas DS showed significantly higher intersection numbers from 2.5 to 3cm from the origin. Intersection number in DS dropped significantly below that of the control treatment between 7.5 and 9cm, while no differences between treatments could be observed between 9.5 and 18cm. For DR the intersection number between 13 and 21cm was almost always significantly lower in the PEG 6000 treatment than that of control treatment. A very strong correlation (r > 0.967) was observed between the manual counting of circle-specific root intersections and the automated counting by Sholl analysis (Fig. 5).


Characterizing root response phenotypes by neural network analysis.

Hatzig SV, Schiessl S, Stahl A, Snowdon RJ - J. Exp. Bot. (2015)

Number of root intersections depending on the distance from root origin of a DR (dotted lines) and a DS (squared lines) winter oilseed rape genotype, measured under control conditions (open symbols) and after 5 days of PEG 6000 treatment (closed symbols). Points are means of five replicates with standard errors (this figure is available in colour at JXB online).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585416&req=5

Figure 4: Number of root intersections depending on the distance from root origin of a DR (dotted lines) and a DS (squared lines) winter oilseed rape genotype, measured under control conditions (open symbols) and after 5 days of PEG 6000 treatment (closed symbols). Points are means of five replicates with standard errors (this figure is available in colour at JXB online).
Mentions: No difference in absolute number of root-circle intersections was observed between the control treatments of the two investigated B. napus genotypes (Fig. 3). Under osmotic stress conditions, however, genotype DR reacted with a significant increase in intersection number, while for genotype DS no difference in intersection number could be observed between treatments. In genotype DR the absolute number of intersections increased from 173 (control) to 258 (PEG 6000), while DS showed 165 intersections under control conditions and 163 after PEG 6000 treatment. Under control conditions the last root-circle intersection in genotype DR occurred at a radius of 26cm, whereas for genotype DS the farthest circle intersection was measured at 21cm from the root origin (Fig. 4).Outermost root intersection under PEG 6000 treatment was measured at radii of 22cm and 18cm for DR and DS, respectively. Under control conditions, both genotypes showed a similar number of root-circle intersections between 0.5 and 4cm distance to the root origin. The number of root intersections was significantly lower at 6.5cm for genotype DR than for DS, whereas it was significantly higher at most circles between 9.5 and 26cm from the origin. Both genotypes showed a significant circle-specific increase in intersection number in the PEG 6000 treatment. In DR the intersection number increased significantly between 3 and 10cm, whereas DS showed significantly higher intersection numbers from 2.5 to 3cm from the origin. Intersection number in DS dropped significantly below that of the control treatment between 7.5 and 9cm, while no differences between treatments could be observed between 9.5 and 18cm. For DR the intersection number between 13 and 21cm was almost always significantly lower in the PEG 6000 treatment than that of control treatment. A very strong correlation (r > 0.967) was observed between the manual counting of circle-specific root intersections and the automated counting by Sholl analysis (Fig. 5).

Bottom Line: Interactive changes in root architecture can be easily captured by individual intersection profiles generated by Sholl analysis.Validation using manual measurements confirmed that the number of lateral roots decreased, while mean lateral root length was enhanced, under osmotic stress conditions.The Sholl methodology is presented as a promising tool for selection of cultivars with advantageous root phenotypes under osmotic stress conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany sarah.hatzig@agrar.uni-giessen.de.

No MeSH data available.