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Characterizing root response phenotypes by neural network analysis.

Hatzig SV, Schiessl S, Stahl A, Snowdon RJ - J. Exp. Bot. (2015)

Bottom Line: Interactive changes in root architecture can be easily captured by individual intersection profiles generated by Sholl analysis.Validation using manual measurements confirmed that the number of lateral roots decreased, while mean lateral root length was enhanced, under osmotic stress conditions.The Sholl methodology is presented as a promising tool for selection of cultivars with advantageous root phenotypes under osmotic stress conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany sarah.hatzig@agrar.uni-giessen.de.

No MeSH data available.


Effect of in vitro osmotic stress on (A) root fresh weights (FW), (B) the absolute number of lateral roots (NLR), (C) the mean length of lateral roots (MLRL), (D) the total root length (RL), (E) the total length of lateral roots (LRL), and (F) the length of the primary root (PRL) of a DR and a DS winter oilseed rape genotype, measured under control conditions (left bars) and after 5 days of PEG 6000 treatment (right bars). Bars show mean measurements of five replicate plants with standard errors indicated by whiskers. Significant differences at #P < 0.1, *P < 0.05, **P < 0.01 and ***P < 0.001 (this figure is available in colour at JXB online).
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Figure 2: Effect of in vitro osmotic stress on (A) root fresh weights (FW), (B) the absolute number of lateral roots (NLR), (C) the mean length of lateral roots (MLRL), (D) the total root length (RL), (E) the total length of lateral roots (LRL), and (F) the length of the primary root (PRL) of a DR and a DS winter oilseed rape genotype, measured under control conditions (left bars) and after 5 days of PEG 6000 treatment (right bars). Bars show mean measurements of five replicate plants with standard errors indicated by whiskers. Significant differences at #P < 0.1, *P < 0.05, **P < 0.01 and ***P < 0.001 (this figure is available in colour at JXB online).

Mentions: For both genotypes no significant differences in root FWs could be observed between control and PEG 6000 treatment (Fig. 2A). For genotype DR the mean root FW was 51.2mg in the control treatment and 60.2mg in the PEG 6000 treatment. For genotype DS a mean root FW of 35.1mg was measured in the control treatment, with 31.5 in the PEG 6000 treatment. Under osmotic stress conditions, NLR decreased significantly in both genotypes (Fig. 2B). In genotype DR, NLR decreased to almost 50% compared to the control, while genotype DS showed a reduction of 40% under stress. In contrast, osmotic stress caused an increase in MLRL in both genotypes. However, significant differences between the treatments could be confirmed for genotype DR only, which showed an increase from 1.48cm (control) to 6.19cm (PEG 6000) (Fig. 2C). Mean values for RL and LRL in both genotypes were increased under stress but not significantly different from the measurements in the control treatment (Fig. 2D, E). PRL was significantly reduced under osmotic stress in genotype DR, while no differences could be observed for DS (Fig. 2F).


Characterizing root response phenotypes by neural network analysis.

Hatzig SV, Schiessl S, Stahl A, Snowdon RJ - J. Exp. Bot. (2015)

Effect of in vitro osmotic stress on (A) root fresh weights (FW), (B) the absolute number of lateral roots (NLR), (C) the mean length of lateral roots (MLRL), (D) the total root length (RL), (E) the total length of lateral roots (LRL), and (F) the length of the primary root (PRL) of a DR and a DS winter oilseed rape genotype, measured under control conditions (left bars) and after 5 days of PEG 6000 treatment (right bars). Bars show mean measurements of five replicate plants with standard errors indicated by whiskers. Significant differences at #P < 0.1, *P < 0.05, **P < 0.01 and ***P < 0.001 (this figure is available in colour at JXB online).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585416&req=5

Figure 2: Effect of in vitro osmotic stress on (A) root fresh weights (FW), (B) the absolute number of lateral roots (NLR), (C) the mean length of lateral roots (MLRL), (D) the total root length (RL), (E) the total length of lateral roots (LRL), and (F) the length of the primary root (PRL) of a DR and a DS winter oilseed rape genotype, measured under control conditions (left bars) and after 5 days of PEG 6000 treatment (right bars). Bars show mean measurements of five replicate plants with standard errors indicated by whiskers. Significant differences at #P < 0.1, *P < 0.05, **P < 0.01 and ***P < 0.001 (this figure is available in colour at JXB online).
Mentions: For both genotypes no significant differences in root FWs could be observed between control and PEG 6000 treatment (Fig. 2A). For genotype DR the mean root FW was 51.2mg in the control treatment and 60.2mg in the PEG 6000 treatment. For genotype DS a mean root FW of 35.1mg was measured in the control treatment, with 31.5 in the PEG 6000 treatment. Under osmotic stress conditions, NLR decreased significantly in both genotypes (Fig. 2B). In genotype DR, NLR decreased to almost 50% compared to the control, while genotype DS showed a reduction of 40% under stress. In contrast, osmotic stress caused an increase in MLRL in both genotypes. However, significant differences between the treatments could be confirmed for genotype DR only, which showed an increase from 1.48cm (control) to 6.19cm (PEG 6000) (Fig. 2C). Mean values for RL and LRL in both genotypes were increased under stress but not significantly different from the measurements in the control treatment (Fig. 2D, E). PRL was significantly reduced under osmotic stress in genotype DR, while no differences could be observed for DS (Fig. 2F).

Bottom Line: Interactive changes in root architecture can be easily captured by individual intersection profiles generated by Sholl analysis.Validation using manual measurements confirmed that the number of lateral roots decreased, while mean lateral root length was enhanced, under osmotic stress conditions.The Sholl methodology is presented as a promising tool for selection of cultivars with advantageous root phenotypes under osmotic stress conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany sarah.hatzig@agrar.uni-giessen.de.

No MeSH data available.