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Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions.

Muscolo A, Junker A, Klukas C, Weigelt-Fischer K, Riewe D, Altmann T - J. Exp. Bot. (2015)

Bottom Line: The effects of salinity and drought on germination and early growth of Castelluccio di Norcia (CAST), Pantelleria (PAN), Ustica (UST), and Eston (EST) accessions were evaluated to identify metabolic and phenotypic traits related to drought and/or salinity stress tolerance.The results showed a relationship between imposed stresses and performance of the cultivars.Stress-specific metabolite indicators were also identified: ornithine and asparagine as markers of drought stress and alanine and homoserine as markers of salinity stress.

View Article: PubMed Central - PubMed

Affiliation: Agriculture Department, Mediterranea University, Feo di Vito, 89124 Reggio Calabria, Italy amuscolo@unirc.it.

No MeSH data available.


Related in: MedlinePlus

Dynamics of the fluorescence signal of four lentil accessions under (A) control (H2O), (B) osmotic stress (PEG), and (C) salt stress (NaCl). Box plots represent variation in fluorescence signal of plants of the different genotypes at day 6, 11, and 15 after sowing (das), with the letters indicating significant differences according to Tukey’s test. (This figure is available in colour at JXB online.)
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Figure 4: Dynamics of the fluorescence signal of four lentil accessions under (A) control (H2O), (B) osmotic stress (PEG), and (C) salt stress (NaCl). Box plots represent variation in fluorescence signal of plants of the different genotypes at day 6, 11, and 15 after sowing (das), with the letters indicating significant differences according to Tukey’s test. (This figure is available in colour at JXB online.)

Mentions: The data on seedling growth were extracted from the images collected from day 6 to day 15 of the cultivation using the IAP software package (Klukas et al., 2014). Under control conditions (Fig. 2, H2O; Fig. 3A), UST was smaller and significantly shorter than the three other genotypes, growth of which was very similar over the entire cultivation period (Fig. 3A), and in respect to side view had lower fluorescence intensity (Fig. 4A) and NIR intensity (displayed as 1–observed grey level) (Fig. 5A). Plant growth (monitored as plant height) was severely reduced in all genotypes upon PEG treatment and, except for the earliest time point [6 days after sowing (DAS)], clear differences were apparent between genotypes, ranking CAST > EST > PAN ≈ UST (Figs 2, 3B). Growth depression was less severe for all four genotypes under NaCl than under PEG treatment. UST was smaller than all other genotypes after a few days of treatment (Figs 2, 3C). Little difference was observed among CAST, PAN, and EST that also showed variation over the treatment period (Fig. 3C). Fluorescence signals (Fig. 4) in all accessions were lower in treated than in untreated conditions and varied particularly strongly in the early phases of both stress treatments; however, values increased over time during the treatments, reaching levels similar to the controls at 15 DAS. Also, NIR intensity values were more variable in the stress treatments than in the control (Fig. 5). The pattern of the genotype ranking according to the NIR intensity values (UST > CAST ≈ PAN ≈ EST) was very similar for the NaCl treatment and the control situation (Fig. 5A, C). In the PEG treatment, however, no significant differences were observed among the accessions.


Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions.

Muscolo A, Junker A, Klukas C, Weigelt-Fischer K, Riewe D, Altmann T - J. Exp. Bot. (2015)

Dynamics of the fluorescence signal of four lentil accessions under (A) control (H2O), (B) osmotic stress (PEG), and (C) salt stress (NaCl). Box plots represent variation in fluorescence signal of plants of the different genotypes at day 6, 11, and 15 after sowing (das), with the letters indicating significant differences according to Tukey’s test. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585415&req=5

Figure 4: Dynamics of the fluorescence signal of four lentil accessions under (A) control (H2O), (B) osmotic stress (PEG), and (C) salt stress (NaCl). Box plots represent variation in fluorescence signal of plants of the different genotypes at day 6, 11, and 15 after sowing (das), with the letters indicating significant differences according to Tukey’s test. (This figure is available in colour at JXB online.)
Mentions: The data on seedling growth were extracted from the images collected from day 6 to day 15 of the cultivation using the IAP software package (Klukas et al., 2014). Under control conditions (Fig. 2, H2O; Fig. 3A), UST was smaller and significantly shorter than the three other genotypes, growth of which was very similar over the entire cultivation period (Fig. 3A), and in respect to side view had lower fluorescence intensity (Fig. 4A) and NIR intensity (displayed as 1–observed grey level) (Fig. 5A). Plant growth (monitored as plant height) was severely reduced in all genotypes upon PEG treatment and, except for the earliest time point [6 days after sowing (DAS)], clear differences were apparent between genotypes, ranking CAST > EST > PAN ≈ UST (Figs 2, 3B). Growth depression was less severe for all four genotypes under NaCl than under PEG treatment. UST was smaller than all other genotypes after a few days of treatment (Figs 2, 3C). Little difference was observed among CAST, PAN, and EST that also showed variation over the treatment period (Fig. 3C). Fluorescence signals (Fig. 4) in all accessions were lower in treated than in untreated conditions and varied particularly strongly in the early phases of both stress treatments; however, values increased over time during the treatments, reaching levels similar to the controls at 15 DAS. Also, NIR intensity values were more variable in the stress treatments than in the control (Fig. 5). The pattern of the genotype ranking according to the NIR intensity values (UST > CAST ≈ PAN ≈ EST) was very similar for the NaCl treatment and the control situation (Fig. 5A, C). In the PEG treatment, however, no significant differences were observed among the accessions.

Bottom Line: The effects of salinity and drought on germination and early growth of Castelluccio di Norcia (CAST), Pantelleria (PAN), Ustica (UST), and Eston (EST) accessions were evaluated to identify metabolic and phenotypic traits related to drought and/or salinity stress tolerance.The results showed a relationship between imposed stresses and performance of the cultivars.Stress-specific metabolite indicators were also identified: ornithine and asparagine as markers of drought stress and alanine and homoserine as markers of salinity stress.

View Article: PubMed Central - PubMed

Affiliation: Agriculture Department, Mediterranea University, Feo di Vito, 89124 Reggio Calabria, Italy amuscolo@unirc.it.

No MeSH data available.


Related in: MedlinePlus