Limits...
Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions.

Muscolo A, Junker A, Klukas C, Weigelt-Fischer K, Riewe D, Altmann T - J. Exp. Bot. (2015)

Bottom Line: The results showed a relationship between imposed stresses and performance of the cultivars.Metabolic differences in the stress tolerance of the different genotypes were related to a reduction in the levels of tricarboxylic acid (TCA) cycle intermediates.The relevant differences, between the most NaCl-tolerant genotype (PAN) and the most sensitive one (EST) were related to the decrease in the threonic acid level.

View Article: PubMed Central - PubMed

Affiliation: Agriculture Department, Mediterranea University, Feo di Vito, 89124 Reggio Calabria, Italy amuscolo@unirc.it.

No MeSH data available.


Related in: MedlinePlus

Representative side view RGB images of plants of four lentil accessions at 15 d after sowing under control conditions (H2O), osmotic stress (PEG), and salt stress (NaCl).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4585415&req=5

Figure 2: Representative side view RGB images of plants of four lentil accessions at 15 d after sowing under control conditions (H2O), osmotic stress (PEG), and salt stress (NaCl).

Mentions: The data on seedling growth were extracted from the images collected from day 6 to day 15 of the cultivation using the IAP software package (Klukas et al., 2014). Under control conditions (Fig. 2, H2O; Fig. 3A), UST was smaller and significantly shorter than the three other genotypes, growth of which was very similar over the entire cultivation period (Fig. 3A), and in respect to side view had lower fluorescence intensity (Fig. 4A) and NIR intensity (displayed as 1–observed grey level) (Fig. 5A). Plant growth (monitored as plant height) was severely reduced in all genotypes upon PEG treatment and, except for the earliest time point [6 days after sowing (DAS)], clear differences were apparent between genotypes, ranking CAST > EST > PAN ≈ UST (Figs 2, 3B). Growth depression was less severe for all four genotypes under NaCl than under PEG treatment. UST was smaller than all other genotypes after a few days of treatment (Figs 2, 3C). Little difference was observed among CAST, PAN, and EST that also showed variation over the treatment period (Fig. 3C). Fluorescence signals (Fig. 4) in all accessions were lower in treated than in untreated conditions and varied particularly strongly in the early phases of both stress treatments; however, values increased over time during the treatments, reaching levels similar to the controls at 15 DAS. Also, NIR intensity values were more variable in the stress treatments than in the control (Fig. 5). The pattern of the genotype ranking according to the NIR intensity values (UST > CAST ≈ PAN ≈ EST) was very similar for the NaCl treatment and the control situation (Fig. 5A, C). In the PEG treatment, however, no significant differences were observed among the accessions.


Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions.

Muscolo A, Junker A, Klukas C, Weigelt-Fischer K, Riewe D, Altmann T - J. Exp. Bot. (2015)

Representative side view RGB images of plants of four lentil accessions at 15 d after sowing under control conditions (H2O), osmotic stress (PEG), and salt stress (NaCl).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585415&req=5

Figure 2: Representative side view RGB images of plants of four lentil accessions at 15 d after sowing under control conditions (H2O), osmotic stress (PEG), and salt stress (NaCl).
Mentions: The data on seedling growth were extracted from the images collected from day 6 to day 15 of the cultivation using the IAP software package (Klukas et al., 2014). Under control conditions (Fig. 2, H2O; Fig. 3A), UST was smaller and significantly shorter than the three other genotypes, growth of which was very similar over the entire cultivation period (Fig. 3A), and in respect to side view had lower fluorescence intensity (Fig. 4A) and NIR intensity (displayed as 1–observed grey level) (Fig. 5A). Plant growth (monitored as plant height) was severely reduced in all genotypes upon PEG treatment and, except for the earliest time point [6 days after sowing (DAS)], clear differences were apparent between genotypes, ranking CAST > EST > PAN ≈ UST (Figs 2, 3B). Growth depression was less severe for all four genotypes under NaCl than under PEG treatment. UST was smaller than all other genotypes after a few days of treatment (Figs 2, 3C). Little difference was observed among CAST, PAN, and EST that also showed variation over the treatment period (Fig. 3C). Fluorescence signals (Fig. 4) in all accessions were lower in treated than in untreated conditions and varied particularly strongly in the early phases of both stress treatments; however, values increased over time during the treatments, reaching levels similar to the controls at 15 DAS. Also, NIR intensity values were more variable in the stress treatments than in the control (Fig. 5). The pattern of the genotype ranking according to the NIR intensity values (UST > CAST ≈ PAN ≈ EST) was very similar for the NaCl treatment and the control situation (Fig. 5A, C). In the PEG treatment, however, no significant differences were observed among the accessions.

Bottom Line: The results showed a relationship between imposed stresses and performance of the cultivars.Metabolic differences in the stress tolerance of the different genotypes were related to a reduction in the levels of tricarboxylic acid (TCA) cycle intermediates.The relevant differences, between the most NaCl-tolerant genotype (PAN) and the most sensitive one (EST) were related to the decrease in the threonic acid level.

View Article: PubMed Central - PubMed

Affiliation: Agriculture Department, Mediterranea University, Feo di Vito, 89124 Reggio Calabria, Italy amuscolo@unirc.it.

No MeSH data available.


Related in: MedlinePlus