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Magnetic resonance imaging of sugar beet taproots in soil reveals growth reduction and morphological changes during foliar Cercospora beticola infestation.

Schmittgen S, Metzner R, Van Dusschoten D, Jansen M, Fiorani F, Jahnke S, Rascher U, Schurr U - J. Exp. Bot. (2015)

Bottom Line: However, during this period, the volumetric growth of the taproot had already started to decrease.Additionally, inoculated plants showed a reduction of the increase in width of inner cambial rings while the width of outer rings increased slightly compared with non-inoculated plants.This response partly compensated for the reduced development of inner rings that had a vascular connection with Cercospora-inoculated leaves.

View Article: PubMed Central - PubMed

Affiliation: Forschungszentrum Jülich GmbH, Institut für Bio-und Geowissenschaften, IBG-2: Plant Sciences, Wilhelm-Johnen-Straße, D-52425 Jülich, Germany simone.schmittgen@julumni.fz-juelich.de s.jahnke@fz-juelich.de.

No MeSH data available.


Related in: MedlinePlus

Root development, measured with MRI, and disease severity of the HS genotype scored visually. (A) Taproot volume (ml) and percentage disease severity at the applicable measurement point with increasing average number of infected leaves per plant (ILPa), 5–19 leaves; asterisks indicate statistically significant differences between plants of the inoculated (+ C.b.) and non-inoculated (– C.b.) treatments estimated by a t-test, P<0.05. (B) Summed thickness of inner (1–4) and outer (5–10) cambial rings between inoculated (+ C.b.) and non-inoculated (– C.b.) treatment. The appearance of cambial rings 9 (c9) and 10 (c10) was delayed in the non-inoculated plants (grey, non-inoculated; black, inoculated). Differences in cambial rings between genotypes were analysed at 98 dpi by one-way ANOVA, P<0.001. Mean values (±SE, n=4 per treatment) were calculated during –14 dpi to 98 dpi (A, B). The two dates of inoculation are indicated by grey arrows.
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Figure 5: Root development, measured with MRI, and disease severity of the HS genotype scored visually. (A) Taproot volume (ml) and percentage disease severity at the applicable measurement point with increasing average number of infected leaves per plant (ILPa), 5–19 leaves; asterisks indicate statistically significant differences between plants of the inoculated (+ C.b.) and non-inoculated (– C.b.) treatments estimated by a t-test, P<0.05. (B) Summed thickness of inner (1–4) and outer (5–10) cambial rings between inoculated (+ C.b.) and non-inoculated (– C.b.) treatment. The appearance of cambial rings 9 (c9) and 10 (c10) was delayed in the non-inoculated plants (grey, non-inoculated; black, inoculated). Differences in cambial rings between genotypes were analysed at 98 dpi by one-way ANOVA, P<0.001. Mean values (±SE, n=4 per treatment) were calculated during –14 dpi to 98 dpi (A, B). The two dates of inoculation are indicated by grey arrows.

Mentions: The inoculated HS plants showed a significantly reduced taproot volume already at 14 dpi compared with the non-inoculated plants (Fig. 5A; t-test with P<0.05), even though the disease severity was low, with only a few leaf spots (<1% infected leaf area; ILPa=5). The second inoculation, 56 d after the first one, resulted in a further reduction of the volumetric taproot growth, with a terminal disease severity of 69% (ILPa=19) at 97 dpi which increased the volumetric taproot difference between treatments (t-test with P<0.05; Fig. 5A). To quantify growth reduction, the RGRs of taproots were compared within 14 d after inoculation. The inoculated plants showed a reduced RGR of 11% (first inoculation) and 13% (second inoculation) compared with the non-inoculated plants (Table 1). At 98 dpi, the inoculated plants had a 28% lower taproot volume as determined by MRI (Fig. 5A) and 26% lower taproot fresh weight that was found gravimetrically (Table 1).


Magnetic resonance imaging of sugar beet taproots in soil reveals growth reduction and morphological changes during foliar Cercospora beticola infestation.

Schmittgen S, Metzner R, Van Dusschoten D, Jansen M, Fiorani F, Jahnke S, Rascher U, Schurr U - J. Exp. Bot. (2015)

Root development, measured with MRI, and disease severity of the HS genotype scored visually. (A) Taproot volume (ml) and percentage disease severity at the applicable measurement point with increasing average number of infected leaves per plant (ILPa), 5–19 leaves; asterisks indicate statistically significant differences between plants of the inoculated (+ C.b.) and non-inoculated (– C.b.) treatments estimated by a t-test, P<0.05. (B) Summed thickness of inner (1–4) and outer (5–10) cambial rings between inoculated (+ C.b.) and non-inoculated (– C.b.) treatment. The appearance of cambial rings 9 (c9) and 10 (c10) was delayed in the non-inoculated plants (grey, non-inoculated; black, inoculated). Differences in cambial rings between genotypes were analysed at 98 dpi by one-way ANOVA, P<0.001. Mean values (±SE, n=4 per treatment) were calculated during –14 dpi to 98 dpi (A, B). The two dates of inoculation are indicated by grey arrows.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Show All Figures
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Figure 5: Root development, measured with MRI, and disease severity of the HS genotype scored visually. (A) Taproot volume (ml) and percentage disease severity at the applicable measurement point with increasing average number of infected leaves per plant (ILPa), 5–19 leaves; asterisks indicate statistically significant differences between plants of the inoculated (+ C.b.) and non-inoculated (– C.b.) treatments estimated by a t-test, P<0.05. (B) Summed thickness of inner (1–4) and outer (5–10) cambial rings between inoculated (+ C.b.) and non-inoculated (– C.b.) treatment. The appearance of cambial rings 9 (c9) and 10 (c10) was delayed in the non-inoculated plants (grey, non-inoculated; black, inoculated). Differences in cambial rings between genotypes were analysed at 98 dpi by one-way ANOVA, P<0.001. Mean values (±SE, n=4 per treatment) were calculated during –14 dpi to 98 dpi (A, B). The two dates of inoculation are indicated by grey arrows.
Mentions: The inoculated HS plants showed a significantly reduced taproot volume already at 14 dpi compared with the non-inoculated plants (Fig. 5A; t-test with P<0.05), even though the disease severity was low, with only a few leaf spots (<1% infected leaf area; ILPa=5). The second inoculation, 56 d after the first one, resulted in a further reduction of the volumetric taproot growth, with a terminal disease severity of 69% (ILPa=19) at 97 dpi which increased the volumetric taproot difference between treatments (t-test with P<0.05; Fig. 5A). To quantify growth reduction, the RGRs of taproots were compared within 14 d after inoculation. The inoculated plants showed a reduced RGR of 11% (first inoculation) and 13% (second inoculation) compared with the non-inoculated plants (Table 1). At 98 dpi, the inoculated plants had a 28% lower taproot volume as determined by MRI (Fig. 5A) and 26% lower taproot fresh weight that was found gravimetrically (Table 1).

Bottom Line: However, during this period, the volumetric growth of the taproot had already started to decrease.Additionally, inoculated plants showed a reduction of the increase in width of inner cambial rings while the width of outer rings increased slightly compared with non-inoculated plants.This response partly compensated for the reduced development of inner rings that had a vascular connection with Cercospora-inoculated leaves.

View Article: PubMed Central - PubMed

Affiliation: Forschungszentrum Jülich GmbH, Institut für Bio-und Geowissenschaften, IBG-2: Plant Sciences, Wilhelm-Johnen-Straße, D-52425 Jülich, Germany simone.schmittgen@julumni.fz-juelich.de s.jahnke@fz-juelich.de.

No MeSH data available.


Related in: MedlinePlus