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Genome-wide association study of rice (Oryza sativa L.) leaf traits with a high-throughput leaf scorer.

Yang W, Guo Z, Huang C, Wang K, Jiang N, Feng H, Chen G, Liu Q, Xiong L - J. Exp. Bot. (2015)

Bottom Line: Nine associated loci contained known leaf-related genes, such as Nal1 for controlling the leaf width.In addition, a total of 73, 123, and 177 new loci were detected for traits associated with leaf size, colour, and shape, respectively.In summary, after evaluating the performance with a large number of rice accessions, the combination of GWAS and high-throughput leaf phenotyping (HLS) has proven a valuable strategy to identify the genetic loci controlling rice leaf traits.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China Agricultural Bioinformatics Key Laboratory of Hubei Province, Huazhong Agricultural University, Wuhan 430070, PR China.

No MeSH data available.


The change in green leaf colour during the three growth stages. The distribution of the GLA ratio at three growth stages as follows: (a) late tillering stage, (b) late booting stage, and (c) milk grain stage. The relationship between the nitrogen content and the GLA ratio: (d) green-2 leaf area ratio, (e) green-3 leaf area ratio, and (f) green-4 leaf area ratio.
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Figure 5: The change in green leaf colour during the three growth stages. The distribution of the GLA ratio at three growth stages as follows: (a) late tillering stage, (b) late booting stage, and (c) milk grain stage. The relationship between the nitrogen content and the GLA ratio: (d) green-2 leaf area ratio, (e) green-3 leaf area ratio, and (f) green-4 leaf area ratio.

Mentions: The GLA shows visible change during the plant life cycle and in response to environmental cues, but the change pattern has seldom been systematically investigated because of measurement difficulties in traditional methods. By taking advantage of HLS, 367 rice accessions at three growth stages (late tillering stage, late booting stage, and milk grain stage) were chosen to analyse the change in the GLA (Fig. 5a–c). The leaf area ratio of the green-3 leaves changed little among the three growth stages, whereas the green-2 leaf area ratio became larger and the green-4 leaf area ratio became smaller with the growth and development of the rice plant, particularly from the late tillering stage to the late booting stage. The ratios of the three classes of green leaves are known to be related to the nitrogen content of the leaves. To investigate the relationship between the leaf nitrogen content and different green leaf colours, 32 rice accessions were randomly chosen and tested with HLS and with auto discrete analyzers (Smartchem200; Alliance Corporation, France). It was found that the green-2 and green-3 leaf area ratio were negatively correlated with the nitrogen content in leaves, and the green-4 leaf area ratio was positively correlated with the nitrogen content in leaves (Fig. 5d–f).


Genome-wide association study of rice (Oryza sativa L.) leaf traits with a high-throughput leaf scorer.

Yang W, Guo Z, Huang C, Wang K, Jiang N, Feng H, Chen G, Liu Q, Xiong L - J. Exp. Bot. (2015)

The change in green leaf colour during the three growth stages. The distribution of the GLA ratio at three growth stages as follows: (a) late tillering stage, (b) late booting stage, and (c) milk grain stage. The relationship between the nitrogen content and the GLA ratio: (d) green-2 leaf area ratio, (e) green-3 leaf area ratio, and (f) green-4 leaf area ratio.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585412&req=5

Figure 5: The change in green leaf colour during the three growth stages. The distribution of the GLA ratio at three growth stages as follows: (a) late tillering stage, (b) late booting stage, and (c) milk grain stage. The relationship between the nitrogen content and the GLA ratio: (d) green-2 leaf area ratio, (e) green-3 leaf area ratio, and (f) green-4 leaf area ratio.
Mentions: The GLA shows visible change during the plant life cycle and in response to environmental cues, but the change pattern has seldom been systematically investigated because of measurement difficulties in traditional methods. By taking advantage of HLS, 367 rice accessions at three growth stages (late tillering stage, late booting stage, and milk grain stage) were chosen to analyse the change in the GLA (Fig. 5a–c). The leaf area ratio of the green-3 leaves changed little among the three growth stages, whereas the green-2 leaf area ratio became larger and the green-4 leaf area ratio became smaller with the growth and development of the rice plant, particularly from the late tillering stage to the late booting stage. The ratios of the three classes of green leaves are known to be related to the nitrogen content of the leaves. To investigate the relationship between the leaf nitrogen content and different green leaf colours, 32 rice accessions were randomly chosen and tested with HLS and with auto discrete analyzers (Smartchem200; Alliance Corporation, France). It was found that the green-2 and green-3 leaf area ratio were negatively correlated with the nitrogen content in leaves, and the green-4 leaf area ratio was positively correlated with the nitrogen content in leaves (Fig. 5d–f).

Bottom Line: Nine associated loci contained known leaf-related genes, such as Nal1 for controlling the leaf width.In addition, a total of 73, 123, and 177 new loci were detected for traits associated with leaf size, colour, and shape, respectively.In summary, after evaluating the performance with a large number of rice accessions, the combination of GWAS and high-throughput leaf phenotyping (HLS) has proven a valuable strategy to identify the genetic loci controlling rice leaf traits.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China Agricultural Bioinformatics Key Laboratory of Hubei Province, Huazhong Agricultural University, Wuhan 430070, PR China.

No MeSH data available.