Limits...
Genome-wide association study of rice (Oryza sativa L.) leaf traits with a high-throughput leaf scorer.

Yang W, Guo Z, Huang C, Wang K, Jiang N, Feng H, Chen G, Liu Q, Xiong L - J. Exp. Bot. (2015)

Bottom Line: Nine associated loci contained known leaf-related genes, such as Nal1 for controlling the leaf width.In addition, a total of 73, 123, and 177 new loci were detected for traits associated with leaf size, colour, and shape, respectively.In summary, after evaluating the performance with a large number of rice accessions, the combination of GWAS and high-throughput leaf phenotyping (HLS) has proven a valuable strategy to identify the genetic loci controlling rice leaf traits.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China Agricultural Bioinformatics Key Laboratory of Hubei Province, Huazhong Agricultural University, Wuhan 430070, PR China.

No MeSH data available.


The design of the HLS system. (a) The developed prototype of the HLS; (b) details of the inspection unit; (c) the configuration drawing of the inspection unit; and (d) the software interface.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4585412&req=5

Figure 1: The design of the HLS system. (a) The developed prototype of the HLS; (b) details of the inspection unit; (c) the configuration drawing of the inspection unit; and (d) the software interface.

Mentions: The HLS system contains two primary elements: a control unit and an inspection unit (Fig. 1a). The control unit is designed for user interaction, system control, image analysis, and data management. The inspection unit is designed for leaf feeding, image scanning, and image transmission. The control unit includes the following three primary devices: a workstation (HP xw6400, Hewlett-Packard Development Company, USA), a camera-link image acquisition device (IMAQ PCI-1428, National Instruments Corporation, USA) that digitizes the images into 3-colour 8-bit files, and a programmable logic controller (PLC, CP1H-Y20DT-D, Omron Corporation, Japan). The details of the operation procedure, data management, and control unit are introduced in Supplementary Video S1, Supplementary Fig. S2, Supplementary Fig. S3, and Supplementary Notes.


Genome-wide association study of rice (Oryza sativa L.) leaf traits with a high-throughput leaf scorer.

Yang W, Guo Z, Huang C, Wang K, Jiang N, Feng H, Chen G, Liu Q, Xiong L - J. Exp. Bot. (2015)

The design of the HLS system. (a) The developed prototype of the HLS; (b) details of the inspection unit; (c) the configuration drawing of the inspection unit; and (d) the software interface.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4585412&req=5

Figure 1: The design of the HLS system. (a) The developed prototype of the HLS; (b) details of the inspection unit; (c) the configuration drawing of the inspection unit; and (d) the software interface.
Mentions: The HLS system contains two primary elements: a control unit and an inspection unit (Fig. 1a). The control unit is designed for user interaction, system control, image analysis, and data management. The inspection unit is designed for leaf feeding, image scanning, and image transmission. The control unit includes the following three primary devices: a workstation (HP xw6400, Hewlett-Packard Development Company, USA), a camera-link image acquisition device (IMAQ PCI-1428, National Instruments Corporation, USA) that digitizes the images into 3-colour 8-bit files, and a programmable logic controller (PLC, CP1H-Y20DT-D, Omron Corporation, Japan). The details of the operation procedure, data management, and control unit are introduced in Supplementary Video S1, Supplementary Fig. S2, Supplementary Fig. S3, and Supplementary Notes.

Bottom Line: Nine associated loci contained known leaf-related genes, such as Nal1 for controlling the leaf width.In addition, a total of 73, 123, and 177 new loci were detected for traits associated with leaf size, colour, and shape, respectively.In summary, after evaluating the performance with a large number of rice accessions, the combination of GWAS and high-throughput leaf phenotyping (HLS) has proven a valuable strategy to identify the genetic loci controlling rice leaf traits.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China Agricultural Bioinformatics Key Laboratory of Hubei Province, Huazhong Agricultural University, Wuhan 430070, PR China.

No MeSH data available.