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Ex vivo tracing of pancreatic neuroendocrine tumors with bio-conjugated fluorescent quantum dots: a paradigm of nanoparticle-based diagnostics.

Giorgakis E, Ramesh B, Loizidou M - Ann Gastroenterol (2015 Oct-Dec)

View Article: PubMed Central - PubMed

Affiliation: Division of Surgery and Interventional Science, University College London (Emmanouil Giorgakis, Bala Ramesh, Marilena Loizidou), London, UK ; Minimal Access and Hepatobiliary Surgery, King's College Hospital NHS Foundation Trust (Emmanouil Giorgakis), London, UK.

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We demonstrated that a novel biomarker, namely calreticulin (CRT), is present in pNETs and may be targeted with fluorescent gold quantum dots (AuQDs)... CRT is a ubiquitous Ca-binding protein with chaperone activity and a rather complex relation to various cancers: its overexpression is positively associated with various solid tumors, usually as an adverse prognostic indicator ; however, contrary to its positive correlation to tumorigenesis and poor cancer prognosis, CRT also seems to promote immunogenic cancer cell death... Illustrations (A) and (B) were captured by LSCM after 543 nm green laser beam excitation via a 650 nanometres/Long Pass (nm/LP) fluorescence barrier, while (C) and (D) after 488 nm blue and 650 nm/LP filtering... There was minimal signal emission after 543 nm excitation due to lower energy confirming NIR emission (A)... Post-incubation with AuQD-antiCRT (B), there was NIR emission (pseudocolor) in 650/LP filter cut-off and therefore CRT expression... Images (C) and (D) also demonstrate NIR fluorescence after AuQD-antiCRT incubation, more intense upon He-Ne (488 nm)... To the authors’ best knowledge, this is the first pictorial demonstration of CRT surface expression on pancreatic neuroendocrine malignancy with the use of fluorescent quantum nanotechnology and tracing of pNETs with bio-stable, non-degradable fluorophores, such as the AuQDs... These findings may facilitate further in vivo investigation on CRT expression on pNETs and on the application of QD–based theranostics on the detection and treatment of these conditions... Finally, these findings should trigger the design of in vivo animal studies on the fluorescence-guided laparoscopic surgery (FGLS) after QD-labeling of CRT-rich solid pancreatic lesions... The advantages of FGLS have already been described by Metildi et al ; the theranostic advantage of QD-fluorophores as both diagnostic and therapeutic tracers and carriers of immunochemical tumoricidal agents and their safety for in vivo applications in the treatment of pNETs are yet to be developed and proven.

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Related in: MedlinePlus

Laser scanning confocal fluorescence microscopy photomicrographs illustrating a pancreatic neuroendocrine tumor before and after exposure to fluorescent gold quantum dots bio-conjugated to anti-calreticulin polyclonal antibodies
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Figure 1: Laser scanning confocal fluorescence microscopy photomicrographs illustrating a pancreatic neuroendocrine tumor before and after exposure to fluorescent gold quantum dots bio-conjugated to anti-calreticulin polyclonal antibodies

Mentions: Among the subjects was a 61-year-old female who underwent a Whipple’s procedure for what proved to be a completely resected, well differentiated neuroendocrine neoplasm, TNM stage pT3N1pMx. The photomicrographs of the pathological tissues under laser excitation before and after incubation to AuQD-antiCRT are depicted in Fig. 1. Illustrations (A) and (B) were captured by LSCM after 543 nm green laser beam excitation via a 650 nanometres/Long Pass (nm/LP) fluorescence barrier, while (C) and (D) after 488 nm blue and 650 nm/LP filtering. There was minimal signal emission after 543 nm excitation due to lower energy confirming NIR emission (A). Post-incubation with AuQD-antiCRT (B), there was NIR emission (pseudocolor) in 650/LP filter cut-off and therefore CRT expression. Images (C) and (D) also demonstrate NIR fluorescence after AuQD-antiCRT incubation, more intense upon He-Ne (488 nm). These two latter photographs also demonstrate some of the architectural appearances typical of pNETs, such as the cell nests (singlearrows), trabeculi (double arrows) and gyriform patterns (arrowheads).


Ex vivo tracing of pancreatic neuroendocrine tumors with bio-conjugated fluorescent quantum dots: a paradigm of nanoparticle-based diagnostics.

Giorgakis E, Ramesh B, Loizidou M - Ann Gastroenterol (2015 Oct-Dec)

Laser scanning confocal fluorescence microscopy photomicrographs illustrating a pancreatic neuroendocrine tumor before and after exposure to fluorescent gold quantum dots bio-conjugated to anti-calreticulin polyclonal antibodies
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585408&req=5

Figure 1: Laser scanning confocal fluorescence microscopy photomicrographs illustrating a pancreatic neuroendocrine tumor before and after exposure to fluorescent gold quantum dots bio-conjugated to anti-calreticulin polyclonal antibodies
Mentions: Among the subjects was a 61-year-old female who underwent a Whipple’s procedure for what proved to be a completely resected, well differentiated neuroendocrine neoplasm, TNM stage pT3N1pMx. The photomicrographs of the pathological tissues under laser excitation before and after incubation to AuQD-antiCRT are depicted in Fig. 1. Illustrations (A) and (B) were captured by LSCM after 543 nm green laser beam excitation via a 650 nanometres/Long Pass (nm/LP) fluorescence barrier, while (C) and (D) after 488 nm blue and 650 nm/LP filtering. There was minimal signal emission after 543 nm excitation due to lower energy confirming NIR emission (A). Post-incubation with AuQD-antiCRT (B), there was NIR emission (pseudocolor) in 650/LP filter cut-off and therefore CRT expression. Images (C) and (D) also demonstrate NIR fluorescence after AuQD-antiCRT incubation, more intense upon He-Ne (488 nm). These two latter photographs also demonstrate some of the architectural appearances typical of pNETs, such as the cell nests (singlearrows), trabeculi (double arrows) and gyriform patterns (arrowheads).

View Article: PubMed Central - PubMed

Affiliation: Division of Surgery and Interventional Science, University College London (Emmanouil Giorgakis, Bala Ramesh, Marilena Loizidou), London, UK ; Minimal Access and Hepatobiliary Surgery, King's College Hospital NHS Foundation Trust (Emmanouil Giorgakis), London, UK.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

We demonstrated that a novel biomarker, namely calreticulin (CRT), is present in pNETs and may be targeted with fluorescent gold quantum dots (AuQDs)... CRT is a ubiquitous Ca-binding protein with chaperone activity and a rather complex relation to various cancers: its overexpression is positively associated with various solid tumors, usually as an adverse prognostic indicator ; however, contrary to its positive correlation to tumorigenesis and poor cancer prognosis, CRT also seems to promote immunogenic cancer cell death... Illustrations (A) and (B) were captured by LSCM after 543 nm green laser beam excitation via a 650 nanometres/Long Pass (nm/LP) fluorescence barrier, while (C) and (D) after 488 nm blue and 650 nm/LP filtering... There was minimal signal emission after 543 nm excitation due to lower energy confirming NIR emission (A)... Post-incubation with AuQD-antiCRT (B), there was NIR emission (pseudocolor) in 650/LP filter cut-off and therefore CRT expression... Images (C) and (D) also demonstrate NIR fluorescence after AuQD-antiCRT incubation, more intense upon He-Ne (488 nm)... To the authors’ best knowledge, this is the first pictorial demonstration of CRT surface expression on pancreatic neuroendocrine malignancy with the use of fluorescent quantum nanotechnology and tracing of pNETs with bio-stable, non-degradable fluorophores, such as the AuQDs... These findings may facilitate further in vivo investigation on CRT expression on pNETs and on the application of QD–based theranostics on the detection and treatment of these conditions... Finally, these findings should trigger the design of in vivo animal studies on the fluorescence-guided laparoscopic surgery (FGLS) after QD-labeling of CRT-rich solid pancreatic lesions... The advantages of FGLS have already been described by Metildi et al ; the theranostic advantage of QD-fluorophores as both diagnostic and therapeutic tracers and carriers of immunochemical tumoricidal agents and their safety for in vivo applications in the treatment of pNETs are yet to be developed and proven.

No MeSH data available.


Related in: MedlinePlus