Limits...
Decreased expression of alpha-2-HS glycoprotein in the sera of rats treated with Eurycoma longifolia extract.

Chen Y, Phang WM, Mu AK, Chan CK, Low BS, Sasidharan S, Chan KL - Front Pharmacol (2015)

Bottom Line: Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear.Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay.AHS expression is known to be associated with insulin resistance and diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya Kuala Lumpur, Malaysia ; Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya Kuala Lumpur, Malaysia.

ABSTRACT
Eurycoma longifolia is a Malaysian native herb that has been widely used as an aphrodisiac and a remedy for andropause. Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear. In the present study, we have investigated the effect of standardized E. longifolia extract on serum protein expression up to 28 days following oral administration in rats. Serum protein profiles were analyzed by 2-dimensional electrophoresis, and altered proteins were identified via mass spectrometry. We observed that alpha-2-HS glycoprotein (AHS) was significantly decreased in the serum of experimentally treated rats compared to pre-treated animals. Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay. AHS expression is known to be associated with insulin resistance and diabetes. Our data indicated that serum AHS was reduced in rats treated with standardized E. longifolia extract, and therefore form a prelude for further investigation into the effects of this natural extract in animal models involving infertility and diabetes.

No MeSH data available.


Related in: MedlinePlus

Mean percentage of inhibition of AHS in the pre-treatment and post-treatment serum samples. Analysis was performed by competitive ELISA. AHS concentrations in the pre-treatment sera were significantly higher than those in the post-treatment samples (days 1, 7, 14, 21, and 28). The amount of AHS present was proportional to the percentage inhibition of substrate hydrolysis. Asterisk denotes statistical significance (p < 0.05) of post-treatment samples compared to that of pre-treatment sample (n = 6).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4585282&req=5

Figure 3: Mean percentage of inhibition of AHS in the pre-treatment and post-treatment serum samples. Analysis was performed by competitive ELISA. AHS concentrations in the pre-treatment sera were significantly higher than those in the post-treatment samples (days 1, 7, 14, 21, and 28). The amount of AHS present was proportional to the percentage inhibition of substrate hydrolysis. Asterisk denotes statistical significance (p < 0.05) of post-treatment samples compared to that of pre-treatment sample (n = 6).

Mentions: To validate AHS reduction upon extract treatment, competitive ELISA was performed on the serum samples (n = 6 for each group; pre-treatment, days 1, 7, 14, 21, and 28) using polyclonal antisera against AHS. We observed that AHS expression was significantly higher in pre-treatment sera compared to post-treatment sera (Figure 3).


Decreased expression of alpha-2-HS glycoprotein in the sera of rats treated with Eurycoma longifolia extract.

Chen Y, Phang WM, Mu AK, Chan CK, Low BS, Sasidharan S, Chan KL - Front Pharmacol (2015)

Mean percentage of inhibition of AHS in the pre-treatment and post-treatment serum samples. Analysis was performed by competitive ELISA. AHS concentrations in the pre-treatment sera were significantly higher than those in the post-treatment samples (days 1, 7, 14, 21, and 28). The amount of AHS present was proportional to the percentage inhibition of substrate hydrolysis. Asterisk denotes statistical significance (p < 0.05) of post-treatment samples compared to that of pre-treatment sample (n = 6).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585282&req=5

Figure 3: Mean percentage of inhibition of AHS in the pre-treatment and post-treatment serum samples. Analysis was performed by competitive ELISA. AHS concentrations in the pre-treatment sera were significantly higher than those in the post-treatment samples (days 1, 7, 14, 21, and 28). The amount of AHS present was proportional to the percentage inhibition of substrate hydrolysis. Asterisk denotes statistical significance (p < 0.05) of post-treatment samples compared to that of pre-treatment sample (n = 6).
Mentions: To validate AHS reduction upon extract treatment, competitive ELISA was performed on the serum samples (n = 6 for each group; pre-treatment, days 1, 7, 14, 21, and 28) using polyclonal antisera against AHS. We observed that AHS expression was significantly higher in pre-treatment sera compared to post-treatment sera (Figure 3).

Bottom Line: Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear.Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay.AHS expression is known to be associated with insulin resistance and diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya Kuala Lumpur, Malaysia ; Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya Kuala Lumpur, Malaysia.

ABSTRACT
Eurycoma longifolia is a Malaysian native herb that has been widely used as an aphrodisiac and a remedy for andropause. Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear. In the present study, we have investigated the effect of standardized E. longifolia extract on serum protein expression up to 28 days following oral administration in rats. Serum protein profiles were analyzed by 2-dimensional electrophoresis, and altered proteins were identified via mass spectrometry. We observed that alpha-2-HS glycoprotein (AHS) was significantly decreased in the serum of experimentally treated rats compared to pre-treated animals. Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay. AHS expression is known to be associated with insulin resistance and diabetes. Our data indicated that serum AHS was reduced in rats treated with standardized E. longifolia extract, and therefore form a prelude for further investigation into the effects of this natural extract in animal models involving infertility and diabetes.

No MeSH data available.


Related in: MedlinePlus