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Decreased expression of alpha-2-HS glycoprotein in the sera of rats treated with Eurycoma longifolia extract.

Chen Y, Phang WM, Mu AK, Chan CK, Low BS, Sasidharan S, Chan KL - Front Pharmacol (2015)

Bottom Line: Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear.Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay.AHS expression is known to be associated with insulin resistance and diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya Kuala Lumpur, Malaysia ; Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya Kuala Lumpur, Malaysia.

ABSTRACT
Eurycoma longifolia is a Malaysian native herb that has been widely used as an aphrodisiac and a remedy for andropause. Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear. In the present study, we have investigated the effect of standardized E. longifolia extract on serum protein expression up to 28 days following oral administration in rats. Serum protein profiles were analyzed by 2-dimensional electrophoresis, and altered proteins were identified via mass spectrometry. We observed that alpha-2-HS glycoprotein (AHS) was significantly decreased in the serum of experimentally treated rats compared to pre-treated animals. Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay. AHS expression is known to be associated with insulin resistance and diabetes. Our data indicated that serum AHS was reduced in rats treated with standardized E. longifolia extract, and therefore form a prelude for further investigation into the effects of this natural extract in animal models involving infertility and diabetes.

No MeSH data available.


Related in: MedlinePlus

Typical 2-DE profiles of rat serum samples. (A–F) of section I illustrate representative 2-DE profiles of rat serum from pre-treatment and days 1, 7, 14, 21, and 28, respectively. The boxed protein cluster corresponds to AHS, which was lost in the serum samples from rats treated with E. longifolia extract (section II displays cropped images). Gel acidity and relative molecular weights are indicated.
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Figure 2: Typical 2-DE profiles of rat serum samples. (A–F) of section I illustrate representative 2-DE profiles of rat serum from pre-treatment and days 1, 7, 14, 21, and 28, respectively. The boxed protein cluster corresponds to AHS, which was lost in the serum samples from rats treated with E. longifolia extract (section II displays cropped images). Gel acidity and relative molecular weights are indicated.

Mentions: Serum proteins from the pre-treatment and treated (days 1, 7, 14, 21, and 28) rat groups were separated by 2-DE on the basis of their isoelectric points (pIs) and molecular weights, yielding complex protein profiles consisting of hundreds of highly-resolved protein spots. Figure 2 illustrates representative serum protein profiles of rats before and after treatment with E. longifolia extract.


Decreased expression of alpha-2-HS glycoprotein in the sera of rats treated with Eurycoma longifolia extract.

Chen Y, Phang WM, Mu AK, Chan CK, Low BS, Sasidharan S, Chan KL - Front Pharmacol (2015)

Typical 2-DE profiles of rat serum samples. (A–F) of section I illustrate representative 2-DE profiles of rat serum from pre-treatment and days 1, 7, 14, 21, and 28, respectively. The boxed protein cluster corresponds to AHS, which was lost in the serum samples from rats treated with E. longifolia extract (section II displays cropped images). Gel acidity and relative molecular weights are indicated.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585282&req=5

Figure 2: Typical 2-DE profiles of rat serum samples. (A–F) of section I illustrate representative 2-DE profiles of rat serum from pre-treatment and days 1, 7, 14, 21, and 28, respectively. The boxed protein cluster corresponds to AHS, which was lost in the serum samples from rats treated with E. longifolia extract (section II displays cropped images). Gel acidity and relative molecular weights are indicated.
Mentions: Serum proteins from the pre-treatment and treated (days 1, 7, 14, 21, and 28) rat groups were separated by 2-DE on the basis of their isoelectric points (pIs) and molecular weights, yielding complex protein profiles consisting of hundreds of highly-resolved protein spots. Figure 2 illustrates representative serum protein profiles of rats before and after treatment with E. longifolia extract.

Bottom Line: Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear.Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay.AHS expression is known to be associated with insulin resistance and diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya Kuala Lumpur, Malaysia ; Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya Kuala Lumpur, Malaysia.

ABSTRACT
Eurycoma longifolia is a Malaysian native herb that has been widely used as an aphrodisiac and a remedy for andropause. Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear. In the present study, we have investigated the effect of standardized E. longifolia extract on serum protein expression up to 28 days following oral administration in rats. Serum protein profiles were analyzed by 2-dimensional electrophoresis, and altered proteins were identified via mass spectrometry. We observed that alpha-2-HS glycoprotein (AHS) was significantly decreased in the serum of experimentally treated rats compared to pre-treated animals. Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay. AHS expression is known to be associated with insulin resistance and diabetes. Our data indicated that serum AHS was reduced in rats treated with standardized E. longifolia extract, and therefore form a prelude for further investigation into the effects of this natural extract in animal models involving infertility and diabetes.

No MeSH data available.


Related in: MedlinePlus