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Structural and metabolic changes in rhizophores of the Cerrado species Chrysolaena obovata (Less.) Dematt. as influenced by drought and re-watering.

Garcia PM, Hayashi AH, Silva EA, Figueiredo-Ribeiro Rde C, Carvalho MA - Front Plant Sci (2015)

Bottom Line: Inulin sphero-crystals accumulated in parenchymatic cells of the cortex, vascular tissues and pith were reduced under drought and accompanied anatomical changes, starting from day 10.At 22 days of drought, the cortical and vascular tissues were collapsed, and inulin sphero-crystals and inulin content were reduced.The localization of inulin sphero-crystals in vascular tissues of C. obovata, as well as the decrease of total inulin and the increase in oligo:polysaccharide ratio in water stressed plants is consistent with the role of fructans in protecting plants against drought.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Fisiologia e Bioquímica, Instituto de Botânica São Paulo, Brazil ; Programa de Pós-Graduação em Biodiversidade Vegetal e Meio Ambiente, Instituto de Botânica São Paulo, Brazil.

ABSTRACT
The high fructan contents in underground organs of Cerrado species, high water solubility, and fast metabolism of these compounds highlight their role as carbon storage and as an adaptive feature in plants under drought. In this study, we showed that anatomical structure, in association with soluble compounds and metabolism of inulin-type fructans were modified in rhizophores of Crysolaena obovata submitted to water suppression and recovery after re-watering. Plants were subjected to daily watering (control), suppression of watering for 22 days (water suppression) and suppression of watering followed by re-watering after 10 days (re-watered). Plants were collected at time 0 and after 3, 7, 10, 12, 17, and 22 days of treatment. In addition to changes in fructan metabolism, high proline content was detected in drought stressed plants, contributing to osmoregulation and recovery after water status reestablishment. Under water suppression, total inulin was reduced from approx. 60 to 40%, mainly due to exohydrolase activity. Concurrently, the activity of fructosyltransferases promoted the production of short chain inulin, which could contribute to the increase in osmotic potential. After re-watering, most parameters analyzed were similar to those of control plants, indicating the resumption of regular metabolism, after water absorption. Inulin sphero-crystals accumulated in parenchymatic cells of the cortex, vascular tissues and pith were reduced under drought and accompanied anatomical changes, starting from day 10. At 22 days of drought, the cortical and vascular tissues were collapsed, and inulin sphero-crystals and inulin content were reduced. The localization of inulin sphero-crystals in vascular tissues of C. obovata, as well as the decrease of total inulin and the increase in oligo:polysaccharide ratio in water stressed plants is consistent with the role of fructans in protecting plants against drought.

No MeSH data available.


Related in: MedlinePlus

Activities of 1-SST (A), 1-FFT (B), 1-FEH (C), and invertase (D) in rhizophores of Chrysolaena obovata submitted to daily watering—control (■), water suppression (○) and re-watering (●). Arrows indicate re-watering at day 10. Values are means ± SE (n = 3). Means followed by *indicate significant differences in relation to control (P = 0.05 by LSD procedure).
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Figure 8: Activities of 1-SST (A), 1-FFT (B), 1-FEH (C), and invertase (D) in rhizophores of Chrysolaena obovata submitted to daily watering—control (■), water suppression (○) and re-watering (●). Arrows indicate re-watering at day 10. Values are means ± SE (n = 3). Means followed by *indicate significant differences in relation to control (P = 0.05 by LSD procedure).

Mentions: 1-SST activity in WS, as well as in control plants, increased initially, indicating fructan synthesis. At the end of the experimental period, 1-SST activity in WS plants was significantly lower than in control and RW plants (Figure 8A). A similar profile was observed for 1-FFT, mainly after day 10, although significantly high 1-FFT activity was observed in WS plants throughout most of the experimental period (Figure 8B). 1-FEH was in general significantly more active in WS plants, declining after re-watering and reaching low levels, close to those found in control plants, on day 22 (Figure 8C). Invertase activity increased notably in WS plants from day 10 on, and decreased after re-watering to values similar to control plants (Figure 8D). The marked increase in invertase activity in WS plants was consistent with the increase in reducing sugars and fructose, detected by HPAEC/PAD (Supplementary Figure 1).


Structural and metabolic changes in rhizophores of the Cerrado species Chrysolaena obovata (Less.) Dematt. as influenced by drought and re-watering.

Garcia PM, Hayashi AH, Silva EA, Figueiredo-Ribeiro Rde C, Carvalho MA - Front Plant Sci (2015)

Activities of 1-SST (A), 1-FFT (B), 1-FEH (C), and invertase (D) in rhizophores of Chrysolaena obovata submitted to daily watering—control (■), water suppression (○) and re-watering (●). Arrows indicate re-watering at day 10. Values are means ± SE (n = 3). Means followed by *indicate significant differences in relation to control (P = 0.05 by LSD procedure).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585265&req=5

Figure 8: Activities of 1-SST (A), 1-FFT (B), 1-FEH (C), and invertase (D) in rhizophores of Chrysolaena obovata submitted to daily watering—control (■), water suppression (○) and re-watering (●). Arrows indicate re-watering at day 10. Values are means ± SE (n = 3). Means followed by *indicate significant differences in relation to control (P = 0.05 by LSD procedure).
Mentions: 1-SST activity in WS, as well as in control plants, increased initially, indicating fructan synthesis. At the end of the experimental period, 1-SST activity in WS plants was significantly lower than in control and RW plants (Figure 8A). A similar profile was observed for 1-FFT, mainly after day 10, although significantly high 1-FFT activity was observed in WS plants throughout most of the experimental period (Figure 8B). 1-FEH was in general significantly more active in WS plants, declining after re-watering and reaching low levels, close to those found in control plants, on day 22 (Figure 8C). Invertase activity increased notably in WS plants from day 10 on, and decreased after re-watering to values similar to control plants (Figure 8D). The marked increase in invertase activity in WS plants was consistent with the increase in reducing sugars and fructose, detected by HPAEC/PAD (Supplementary Figure 1).

Bottom Line: Inulin sphero-crystals accumulated in parenchymatic cells of the cortex, vascular tissues and pith were reduced under drought and accompanied anatomical changes, starting from day 10.At 22 days of drought, the cortical and vascular tissues were collapsed, and inulin sphero-crystals and inulin content were reduced.The localization of inulin sphero-crystals in vascular tissues of C. obovata, as well as the decrease of total inulin and the increase in oligo:polysaccharide ratio in water stressed plants is consistent with the role of fructans in protecting plants against drought.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Fisiologia e Bioquímica, Instituto de Botânica São Paulo, Brazil ; Programa de Pós-Graduação em Biodiversidade Vegetal e Meio Ambiente, Instituto de Botânica São Paulo, Brazil.

ABSTRACT
The high fructan contents in underground organs of Cerrado species, high water solubility, and fast metabolism of these compounds highlight their role as carbon storage and as an adaptive feature in plants under drought. In this study, we showed that anatomical structure, in association with soluble compounds and metabolism of inulin-type fructans were modified in rhizophores of Crysolaena obovata submitted to water suppression and recovery after re-watering. Plants were subjected to daily watering (control), suppression of watering for 22 days (water suppression) and suppression of watering followed by re-watering after 10 days (re-watered). Plants were collected at time 0 and after 3, 7, 10, 12, 17, and 22 days of treatment. In addition to changes in fructan metabolism, high proline content was detected in drought stressed plants, contributing to osmoregulation and recovery after water status reestablishment. Under water suppression, total inulin was reduced from approx. 60 to 40%, mainly due to exohydrolase activity. Concurrently, the activity of fructosyltransferases promoted the production of short chain inulin, which could contribute to the increase in osmotic potential. After re-watering, most parameters analyzed were similar to those of control plants, indicating the resumption of regular metabolism, after water absorption. Inulin sphero-crystals accumulated in parenchymatic cells of the cortex, vascular tissues and pith were reduced under drought and accompanied anatomical changes, starting from day 10. At 22 days of drought, the cortical and vascular tissues were collapsed, and inulin sphero-crystals and inulin content were reduced. The localization of inulin sphero-crystals in vascular tissues of C. obovata, as well as the decrease of total inulin and the increase in oligo:polysaccharide ratio in water stressed plants is consistent with the role of fructans in protecting plants against drought.

No MeSH data available.


Related in: MedlinePlus