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Characterization and expression profile of CaNAC2 pepper gene.

Guo WL, Wang SB, Chen RG, Chen BH, Du XH, Yin YX, Gong ZH, Zhang YY - Front Plant Sci (2015)

Bottom Line: Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids.CaNAC2 was expressed mainly in seed and root.These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University Yangling, China ; School of Horticulture Landscape Architecture, Henan Institute of Science and Technology Xinxiang, China.

ABSTRACT
The plant-specific NAC (NAM, ATAF, and CUC) transcription factors have diverse role in development and stress regulation. A new transcript encoding NAC protein, homologous to nam-like protein 4 from Petunia was identified from an ABA-regulated subtractive cDNA library of Capsicum annuum seedling. Here, this homolog (named CaNAC2) from C. annuum was characterized and investigated its role in abiotic stress tolerance. Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids. Phylogenetic analysis showed that CaNAC2 belonged to the NAC2 subgroup of the orthologous group 4d. The protein CaNAC2 was subcellularly localized in the nucleus and it had transcriptional activity in yeast cell. CaNAC2 was expressed mainly in seed and root. The transcription expression of CaNAC2 was strongly induced by cold, salt and ABA treatment and inhibited by osmotic stress and SA treatment. Silence of CaNAC2 in virus-induced gene silenced pepper seedlings resulted in the increased susceptibility to cold stress and delayed the salt-induced leaf chlorophyll degradation. These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

No MeSH data available.


Related in: MedlinePlus

CaNAC2-silenced pepper plants. (A) The expression of CaNAC2 in gene-silenced pepper (TRV2: CaNAC2) cv P70 and control plants (TRV2:00) were tested at 45 days after inoculation; (B) Effect of ABA application and chilling stress on lipid peroxidation (MDA) content in CaNAC2-silenced pepper seedlings. (C) Phenotypes analysis of the CaNAC2-silenced and non-silenced pepper seedlings under 6°C cold stress for 6 h. (D) Chlorophyll contents of leaf disks. (E) Leaf disks phenotypes. Leaf disks from the gene-silenced plants were floated in 300 mM NaCl solutions for 72 h at 25°C. Bars with different lower case letters indicate significant differences compared to empty vector control leaves (Student’s t-test, p ≤ 0.05).
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Figure 4: CaNAC2-silenced pepper plants. (A) The expression of CaNAC2 in gene-silenced pepper (TRV2: CaNAC2) cv P70 and control plants (TRV2:00) were tested at 45 days after inoculation; (B) Effect of ABA application and chilling stress on lipid peroxidation (MDA) content in CaNAC2-silenced pepper seedlings. (C) Phenotypes analysis of the CaNAC2-silenced and non-silenced pepper seedlings under 6°C cold stress for 6 h. (D) Chlorophyll contents of leaf disks. (E) Leaf disks phenotypes. Leaf disks from the gene-silenced plants were floated in 300 mM NaCl solutions for 72 h at 25°C. Bars with different lower case letters indicate significant differences compared to empty vector control leaves (Student’s t-test, p ≤ 0.05).

Mentions: Compared to the negative control (inoculated with TRV2), the CaNAC2 silencing rate reached nearly 72% (Figure 4A), suggesting that VIGS was successful and effective for CaNAC2 gene silencing in pepper. About 35 silenced pepper seedlings were used for chilling stress, ABA pre-treatment followed by chilling stress or salt stress. Loss-of-function of CaNAC2 in pepper plants increased susceptibility to chilling stress, the lipid peroxidation (MDA) content of the CaNAC2-silenced leaves was significantly higher than that of the empty vector control plants. Chilling stress after pretreated with ABA, there is no significant difference between the negative control and the silenced pepper in the MDA content (Figure 4B). Obvious wilting appeared after 6 h of chilling stress in CaNAC2-silenced plants, while control leaves did not exhibit withering (Figure 4C). Responding to 300 mM salt stress after 72 h, the chlorophyll content of the CaNAC2-silenced leaf disks was significantly higher than that of the negative control plants (Figure 4D) and the leaf disks of CaNAC2-silenced pepper stayed green while the negative control appeared loss of chlorophyll (Figure 4E).


Characterization and expression profile of CaNAC2 pepper gene.

Guo WL, Wang SB, Chen RG, Chen BH, Du XH, Yin YX, Gong ZH, Zhang YY - Front Plant Sci (2015)

CaNAC2-silenced pepper plants. (A) The expression of CaNAC2 in gene-silenced pepper (TRV2: CaNAC2) cv P70 and control plants (TRV2:00) were tested at 45 days after inoculation; (B) Effect of ABA application and chilling stress on lipid peroxidation (MDA) content in CaNAC2-silenced pepper seedlings. (C) Phenotypes analysis of the CaNAC2-silenced and non-silenced pepper seedlings under 6°C cold stress for 6 h. (D) Chlorophyll contents of leaf disks. (E) Leaf disks phenotypes. Leaf disks from the gene-silenced plants were floated in 300 mM NaCl solutions for 72 h at 25°C. Bars with different lower case letters indicate significant differences compared to empty vector control leaves (Student’s t-test, p ≤ 0.05).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4585251&req=5

Figure 4: CaNAC2-silenced pepper plants. (A) The expression of CaNAC2 in gene-silenced pepper (TRV2: CaNAC2) cv P70 and control plants (TRV2:00) were tested at 45 days after inoculation; (B) Effect of ABA application and chilling stress on lipid peroxidation (MDA) content in CaNAC2-silenced pepper seedlings. (C) Phenotypes analysis of the CaNAC2-silenced and non-silenced pepper seedlings under 6°C cold stress for 6 h. (D) Chlorophyll contents of leaf disks. (E) Leaf disks phenotypes. Leaf disks from the gene-silenced plants were floated in 300 mM NaCl solutions for 72 h at 25°C. Bars with different lower case letters indicate significant differences compared to empty vector control leaves (Student’s t-test, p ≤ 0.05).
Mentions: Compared to the negative control (inoculated with TRV2), the CaNAC2 silencing rate reached nearly 72% (Figure 4A), suggesting that VIGS was successful and effective for CaNAC2 gene silencing in pepper. About 35 silenced pepper seedlings were used for chilling stress, ABA pre-treatment followed by chilling stress or salt stress. Loss-of-function of CaNAC2 in pepper plants increased susceptibility to chilling stress, the lipid peroxidation (MDA) content of the CaNAC2-silenced leaves was significantly higher than that of the empty vector control plants. Chilling stress after pretreated with ABA, there is no significant difference between the negative control and the silenced pepper in the MDA content (Figure 4B). Obvious wilting appeared after 6 h of chilling stress in CaNAC2-silenced plants, while control leaves did not exhibit withering (Figure 4C). Responding to 300 mM salt stress after 72 h, the chlorophyll content of the CaNAC2-silenced leaf disks was significantly higher than that of the negative control plants (Figure 4D) and the leaf disks of CaNAC2-silenced pepper stayed green while the negative control appeared loss of chlorophyll (Figure 4E).

Bottom Line: Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids.CaNAC2 was expressed mainly in seed and root.These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University Yangling, China ; School of Horticulture Landscape Architecture, Henan Institute of Science and Technology Xinxiang, China.

ABSTRACT
The plant-specific NAC (NAM, ATAF, and CUC) transcription factors have diverse role in development and stress regulation. A new transcript encoding NAC protein, homologous to nam-like protein 4 from Petunia was identified from an ABA-regulated subtractive cDNA library of Capsicum annuum seedling. Here, this homolog (named CaNAC2) from C. annuum was characterized and investigated its role in abiotic stress tolerance. Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids. Phylogenetic analysis showed that CaNAC2 belonged to the NAC2 subgroup of the orthologous group 4d. The protein CaNAC2 was subcellularly localized in the nucleus and it had transcriptional activity in yeast cell. CaNAC2 was expressed mainly in seed and root. The transcription expression of CaNAC2 was strongly induced by cold, salt and ABA treatment and inhibited by osmotic stress and SA treatment. Silence of CaNAC2 in virus-induced gene silenced pepper seedlings resulted in the increased susceptibility to cold stress and delayed the salt-induced leaf chlorophyll degradation. These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

No MeSH data available.


Related in: MedlinePlus