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Characterization and expression profile of CaNAC2 pepper gene.

Guo WL, Wang SB, Chen RG, Chen BH, Du XH, Yin YX, Gong ZH, Zhang YY - Front Plant Sci (2015)

Bottom Line: Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids.CaNAC2 was expressed mainly in seed and root.These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University Yangling, China ; School of Horticulture Landscape Architecture, Henan Institute of Science and Technology Xinxiang, China.

ABSTRACT
The plant-specific NAC (NAM, ATAF, and CUC) transcription factors have diverse role in development and stress regulation. A new transcript encoding NAC protein, homologous to nam-like protein 4 from Petunia was identified from an ABA-regulated subtractive cDNA library of Capsicum annuum seedling. Here, this homolog (named CaNAC2) from C. annuum was characterized and investigated its role in abiotic stress tolerance. Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids. Phylogenetic analysis showed that CaNAC2 belonged to the NAC2 subgroup of the orthologous group 4d. The protein CaNAC2 was subcellularly localized in the nucleus and it had transcriptional activity in yeast cell. CaNAC2 was expressed mainly in seed and root. The transcription expression of CaNAC2 was strongly induced by cold, salt and ABA treatment and inhibited by osmotic stress and SA treatment. Silence of CaNAC2 in virus-induced gene silenced pepper seedlings resulted in the increased susceptibility to cold stress and delayed the salt-induced leaf chlorophyll degradation. These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

No MeSH data available.


Related in: MedlinePlus

CaNAC2 expression both in tissue and in response to stress and exogenous hormones. (A) Tissue specific expression of CaNAC2; (B) The pepper seedlings were exposed to cold stress (6°C), (C) salt stress (300 mM NaCl) and (D) osmotic stress (300 mM mannitol) for the indicated times; the pepper seedlings were sprayed with exogenous ABA (E) and SA (F). Pepper CaUbi3 gene was used as an internal control for qRT-PCR. The transcript level of CaNAC2 at 0 h is used as control (quantities of calibrator) and was assumed as 1. Results are the mean ± SE. Different letters indicate that the mean values are different by the Tukey HSD test (p ≤ 0.05). Each mean was compared with all the other mean values shown in the same figure.
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Figure 3: CaNAC2 expression both in tissue and in response to stress and exogenous hormones. (A) Tissue specific expression of CaNAC2; (B) The pepper seedlings were exposed to cold stress (6°C), (C) salt stress (300 mM NaCl) and (D) osmotic stress (300 mM mannitol) for the indicated times; the pepper seedlings were sprayed with exogenous ABA (E) and SA (F). Pepper CaUbi3 gene was used as an internal control for qRT-PCR. The transcript level of CaNAC2 at 0 h is used as control (quantities of calibrator) and was assumed as 1. Results are the mean ± SE. Different letters indicate that the mean values are different by the Tukey HSD test (p ≤ 0.05). Each mean was compared with all the other mean values shown in the same figure.

Mentions: The expression pattern of CaNAC2 in different pepper tissues were detected by qRT-PCR. This analysis revealed that the transcription of CaNAC2 was abundant in root and seed, low in stem, leaf, flower, and fruit (Figure 3A).


Characterization and expression profile of CaNAC2 pepper gene.

Guo WL, Wang SB, Chen RG, Chen BH, Du XH, Yin YX, Gong ZH, Zhang YY - Front Plant Sci (2015)

CaNAC2 expression both in tissue and in response to stress and exogenous hormones. (A) Tissue specific expression of CaNAC2; (B) The pepper seedlings were exposed to cold stress (6°C), (C) salt stress (300 mM NaCl) and (D) osmotic stress (300 mM mannitol) for the indicated times; the pepper seedlings were sprayed with exogenous ABA (E) and SA (F). Pepper CaUbi3 gene was used as an internal control for qRT-PCR. The transcript level of CaNAC2 at 0 h is used as control (quantities of calibrator) and was assumed as 1. Results are the mean ± SE. Different letters indicate that the mean values are different by the Tukey HSD test (p ≤ 0.05). Each mean was compared with all the other mean values shown in the same figure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585251&req=5

Figure 3: CaNAC2 expression both in tissue and in response to stress and exogenous hormones. (A) Tissue specific expression of CaNAC2; (B) The pepper seedlings were exposed to cold stress (6°C), (C) salt stress (300 mM NaCl) and (D) osmotic stress (300 mM mannitol) for the indicated times; the pepper seedlings were sprayed with exogenous ABA (E) and SA (F). Pepper CaUbi3 gene was used as an internal control for qRT-PCR. The transcript level of CaNAC2 at 0 h is used as control (quantities of calibrator) and was assumed as 1. Results are the mean ± SE. Different letters indicate that the mean values are different by the Tukey HSD test (p ≤ 0.05). Each mean was compared with all the other mean values shown in the same figure.
Mentions: The expression pattern of CaNAC2 in different pepper tissues were detected by qRT-PCR. This analysis revealed that the transcription of CaNAC2 was abundant in root and seed, low in stem, leaf, flower, and fruit (Figure 3A).

Bottom Line: Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids.CaNAC2 was expressed mainly in seed and root.These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University Yangling, China ; School of Horticulture Landscape Architecture, Henan Institute of Science and Technology Xinxiang, China.

ABSTRACT
The plant-specific NAC (NAM, ATAF, and CUC) transcription factors have diverse role in development and stress regulation. A new transcript encoding NAC protein, homologous to nam-like protein 4 from Petunia was identified from an ABA-regulated subtractive cDNA library of Capsicum annuum seedling. Here, this homolog (named CaNAC2) from C. annuum was characterized and investigated its role in abiotic stress tolerance. Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids. Phylogenetic analysis showed that CaNAC2 belonged to the NAC2 subgroup of the orthologous group 4d. The protein CaNAC2 was subcellularly localized in the nucleus and it had transcriptional activity in yeast cell. CaNAC2 was expressed mainly in seed and root. The transcription expression of CaNAC2 was strongly induced by cold, salt and ABA treatment and inhibited by osmotic stress and SA treatment. Silence of CaNAC2 in virus-induced gene silenced pepper seedlings resulted in the increased susceptibility to cold stress and delayed the salt-induced leaf chlorophyll degradation. These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

No MeSH data available.


Related in: MedlinePlus