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Characterization and expression profile of CaNAC2 pepper gene.

Guo WL, Wang SB, Chen RG, Chen BH, Du XH, Yin YX, Gong ZH, Zhang YY - Front Plant Sci (2015)

Bottom Line: Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids.CaNAC2 was expressed mainly in seed and root.These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University Yangling, China ; School of Horticulture Landscape Architecture, Henan Institute of Science and Technology Xinxiang, China.

ABSTRACT
The plant-specific NAC (NAM, ATAF, and CUC) transcription factors have diverse role in development and stress regulation. A new transcript encoding NAC protein, homologous to nam-like protein 4 from Petunia was identified from an ABA-regulated subtractive cDNA library of Capsicum annuum seedling. Here, this homolog (named CaNAC2) from C. annuum was characterized and investigated its role in abiotic stress tolerance. Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids. Phylogenetic analysis showed that CaNAC2 belonged to the NAC2 subgroup of the orthologous group 4d. The protein CaNAC2 was subcellularly localized in the nucleus and it had transcriptional activity in yeast cell. CaNAC2 was expressed mainly in seed and root. The transcription expression of CaNAC2 was strongly induced by cold, salt and ABA treatment and inhibited by osmotic stress and SA treatment. Silence of CaNAC2 in virus-induced gene silenced pepper seedlings resulted in the increased susceptibility to cold stress and delayed the salt-induced leaf chlorophyll degradation. These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

No MeSH data available.


Related in: MedlinePlus

A Neighbor-joining phylogenetic tree of CaNAC2 and NAC proteins from different plant species. The GenBank accession No. of NAC proteins are shown in the brackets. The CaNAC2 protein is marked with arrow. The NAC subgroup names are shown to the right of square.
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Figure 1: A Neighbor-joining phylogenetic tree of CaNAC2 and NAC proteins from different plant species. The GenBank accession No. of NAC proteins are shown in the brackets. The CaNAC2 protein is marked with arrow. The NAC subgroup names are shown to the right of square.

Mentions: A phylogenetic tree was conducted between the overall amino acid sequences of CaNAC2 and other known NAC-domain proteins. CaNAC2 was clustered into the NAC2 subgroup (Figure 1) and highly homologous to NH4 protein from Petunia (84%, identity). Whereas, CaNAC2 was low homologous to ANAC053, ANAC050, and NAC2 from Arabidopsis, with similarity of 30, 42, and 26%, respectively (data not shown).


Characterization and expression profile of CaNAC2 pepper gene.

Guo WL, Wang SB, Chen RG, Chen BH, Du XH, Yin YX, Gong ZH, Zhang YY - Front Plant Sci (2015)

A Neighbor-joining phylogenetic tree of CaNAC2 and NAC proteins from different plant species. The GenBank accession No. of NAC proteins are shown in the brackets. The CaNAC2 protein is marked with arrow. The NAC subgroup names are shown to the right of square.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585251&req=5

Figure 1: A Neighbor-joining phylogenetic tree of CaNAC2 and NAC proteins from different plant species. The GenBank accession No. of NAC proteins are shown in the brackets. The CaNAC2 protein is marked with arrow. The NAC subgroup names are shown to the right of square.
Mentions: A phylogenetic tree was conducted between the overall amino acid sequences of CaNAC2 and other known NAC-domain proteins. CaNAC2 was clustered into the NAC2 subgroup (Figure 1) and highly homologous to NH4 protein from Petunia (84%, identity). Whereas, CaNAC2 was low homologous to ANAC053, ANAC050, and NAC2 from Arabidopsis, with similarity of 30, 42, and 26%, respectively (data not shown).

Bottom Line: Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids.CaNAC2 was expressed mainly in seed and root.These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University Yangling, China ; School of Horticulture Landscape Architecture, Henan Institute of Science and Technology Xinxiang, China.

ABSTRACT
The plant-specific NAC (NAM, ATAF, and CUC) transcription factors have diverse role in development and stress regulation. A new transcript encoding NAC protein, homologous to nam-like protein 4 from Petunia was identified from an ABA-regulated subtractive cDNA library of Capsicum annuum seedling. Here, this homolog (named CaNAC2) from C. annuum was characterized and investigated its role in abiotic stress tolerance. Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids. Phylogenetic analysis showed that CaNAC2 belonged to the NAC2 subgroup of the orthologous group 4d. The protein CaNAC2 was subcellularly localized in the nucleus and it had transcriptional activity in yeast cell. CaNAC2 was expressed mainly in seed and root. The transcription expression of CaNAC2 was strongly induced by cold, salt and ABA treatment and inhibited by osmotic stress and SA treatment. Silence of CaNAC2 in virus-induced gene silenced pepper seedlings resulted in the increased susceptibility to cold stress and delayed the salt-induced leaf chlorophyll degradation. These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.

No MeSH data available.


Related in: MedlinePlus